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Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx. Evidence for similarities in store-operated and calcium release-activated calcium channel components.
J Biol Chem. 2007 Mar 23; 282(12):9105-16.JB

Abstract

Store-operated calcium entry (SOCE) is a ubiquitous mechanism that is mediated by distinct SOC channels, ranging from the highly selective calcium release-activated Ca2+ (CRAC) channel in rat basophilic leukemia and other hematopoietic cells to relatively Ca2+-selective or non-selective SOC channels in other cells. Although the exact composition of these channels is not yet established, TRPC1 contributes to SOC channels and regulation of physiological function of a variety of cell types. Recently, Orai1 and STIM1 have been suggested to be sufficient for generating CRAC channels. Here we show that Orai1 and STIM1 are also required for TRPC1-SOC channels. Knockdown of TRPC1, Orai1, or STIM1 attenuated, whereas overexpression of TRPC1, but not Orai1 or STIM1, induced an increase in SOC entry and I(SOC) in human salivary gland cells. All three proteins were co-localized in the plasma membrane region of cells, and thapsigargin increased co-immunoprecipitation of TRPC1 with STIM1, and Orai1 in human salivary gland cells as well as dispersed mouse submandibular gland cells. In aggregate, the data presented here reveal that all three proteins are essential for generation of I(SOC) in these cells and that dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in activation of SOC channel in response to internal Ca2+ store depletion. Thus, these data suggest a common molecular basis for SOC and CRAC channels.

Authors+Show Affiliations

Secretory Physiology Section, Gene Therapy and Therapeutics Branch, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17224452

Citation

Ong, Hwei Ling, et al. "Dynamic Assembly of TRPC1-STIM1-Orai1 Ternary Complex Is Involved in Store-operated Calcium Influx. Evidence for Similarities in Store-operated and Calcium Release-activated Calcium Channel Components." The Journal of Biological Chemistry, vol. 282, no. 12, 2007, pp. 9105-16.
Ong HL, Cheng KT, Liu X, et al. Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx. Evidence for similarities in store-operated and calcium release-activated calcium channel components. J Biol Chem. 2007;282(12):9105-16.
Ong, H. L., Cheng, K. T., Liu, X., Bandyopadhyay, B. C., Paria, B. C., Soboloff, J., Pani, B., Gwack, Y., Srikanth, S., Singh, B. B., Gill, D. L., Gill, D., & Ambudkar, I. S. (2007). Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx. Evidence for similarities in store-operated and calcium release-activated calcium channel components. The Journal of Biological Chemistry, 282(12), 9105-16.
Ong HL, et al. Dynamic Assembly of TRPC1-STIM1-Orai1 Ternary Complex Is Involved in Store-operated Calcium Influx. Evidence for Similarities in Store-operated and Calcium Release-activated Calcium Channel Components. J Biol Chem. 2007 Mar 23;282(12):9105-16. PubMed PMID: 17224452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx. Evidence for similarities in store-operated and calcium release-activated calcium channel components. AU - Ong,Hwei Ling, AU - Cheng,Kwong Tai, AU - Liu,Xibao, AU - Bandyopadhyay,Bidhan C, AU - Paria,Biman C, AU - Soboloff,Jonathan, AU - Pani,Biswaranjan, AU - Gwack,Yousang, AU - Srikanth,Sonal, AU - Singh,Brij B, AU - Gill,Donald L, AU - Gill,Donald, AU - Ambudkar,Indu S, Y1 - 2007/01/15/ PY - 2007/1/17/pubmed PY - 2007/5/3/medline PY - 2007/1/17/entrez SP - 9105 EP - 16 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 282 IS - 12 N2 - Store-operated calcium entry (SOCE) is a ubiquitous mechanism that is mediated by distinct SOC channels, ranging from the highly selective calcium release-activated Ca2+ (CRAC) channel in rat basophilic leukemia and other hematopoietic cells to relatively Ca2+-selective or non-selective SOC channels in other cells. Although the exact composition of these channels is not yet established, TRPC1 contributes to SOC channels and regulation of physiological function of a variety of cell types. Recently, Orai1 and STIM1 have been suggested to be sufficient for generating CRAC channels. Here we show that Orai1 and STIM1 are also required for TRPC1-SOC channels. Knockdown of TRPC1, Orai1, or STIM1 attenuated, whereas overexpression of TRPC1, but not Orai1 or STIM1, induced an increase in SOC entry and I(SOC) in human salivary gland cells. All three proteins were co-localized in the plasma membrane region of cells, and thapsigargin increased co-immunoprecipitation of TRPC1 with STIM1, and Orai1 in human salivary gland cells as well as dispersed mouse submandibular gland cells. In aggregate, the data presented here reveal that all three proteins are essential for generation of I(SOC) in these cells and that dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in activation of SOC channel in response to internal Ca2+ store depletion. Thus, these data suggest a common molecular basis for SOC and CRAC channels. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/17224452/Dynamic_assembly_of_TRPC1_STIM1_Orai1_ternary_complex_is_involved_in_store_operated_calcium_influx__Evidence_for_similarities_in_store_operated_and_calcium_release_activated_calcium_channel_components_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=17224452 DB - PRIME DP - Unbound Medicine ER -