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Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland).
Environ Microbiol. 2007 Jan; 9(1):26-38.EM

Abstract

We investigated the genotypic diversity of oxygenic and anoxygenic phototrophic microorganisms in microbial mat samples collected from three hot spring localities on the east coast of Greenland. These hot springs harbour unique Arctic microbial ecosystems that have never been studied in detail before. Specific oligonucleotide primers for cyanobacteria, purple sulfur bacteria, green sulfur bacteria and Choroflexus/Roseiflexus-like green non-sulfur bacteria were used for the selective amplification of 16S rRNA gene fragments. Amplification products were separated by denaturing gradient gel electrophoresis (DGGE) and sequenced. In addition, several cyanobacteria were isolated from the mat samples, and classified morphologically and by 16S rRNA-based methods. The cyanobacterial 16S rRNA sequences obtained from DGGE represented a diverse, polyphyletic collection of cyanobacteria. The microbial mat communities were dominated by heterocystous and non-heterocystous filamentous cyanobacteria. Our results indicate that the cyanobacterial community composition in the samples were different for each sampling site. Different layers of the same heterogeneous mat often contained distinct and different communities of cyanobacteria. We observed a relationship between the cyanobacterial community composition and the in situ temperatures of different mat parts. The Greenland mats exhibited a low diversity of anoxygenic phototrophs as compared with other hot spring mats which is possibly related to the photochemical conditions within the mats resulting from the Arctic light regime.

Authors+Show Affiliations

Department of Biotechnology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, the Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17227409

Citation

Roeselers, Guus, et al. "Diversity of Phototrophic Bacteria in Microbial Mats From Arctic Hot Springs (Greenland)." Environmental Microbiology, vol. 9, no. 1, 2007, pp. 26-38.
Roeselers G, Norris TB, Castenholz RW, et al. Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland). Environ Microbiol. 2007;9(1):26-38.
Roeselers, G., Norris, T. B., Castenholz, R. W., Rysgaard, S., Glud, R. N., Kühl, M., & Muyzer, G. (2007). Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland). Environmental Microbiology, 9(1), 26-38.
Roeselers G, et al. Diversity of Phototrophic Bacteria in Microbial Mats From Arctic Hot Springs (Greenland). Environ Microbiol. 2007;9(1):26-38. PubMed PMID: 17227409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland). AU - Roeselers,Guus, AU - Norris,Tracy B, AU - Castenholz,Richard W, AU - Rysgaard,Søren, AU - Glud,Ronnie N, AU - Kühl,Michael, AU - Muyzer,Gerard, PY - 2007/1/18/pubmed PY - 2007/7/3/medline PY - 2007/1/18/entrez SP - 26 EP - 38 JF - Environmental microbiology JO - Environ Microbiol VL - 9 IS - 1 N2 - We investigated the genotypic diversity of oxygenic and anoxygenic phototrophic microorganisms in microbial mat samples collected from three hot spring localities on the east coast of Greenland. These hot springs harbour unique Arctic microbial ecosystems that have never been studied in detail before. Specific oligonucleotide primers for cyanobacteria, purple sulfur bacteria, green sulfur bacteria and Choroflexus/Roseiflexus-like green non-sulfur bacteria were used for the selective amplification of 16S rRNA gene fragments. Amplification products were separated by denaturing gradient gel electrophoresis (DGGE) and sequenced. In addition, several cyanobacteria were isolated from the mat samples, and classified morphologically and by 16S rRNA-based methods. The cyanobacterial 16S rRNA sequences obtained from DGGE represented a diverse, polyphyletic collection of cyanobacteria. The microbial mat communities were dominated by heterocystous and non-heterocystous filamentous cyanobacteria. Our results indicate that the cyanobacterial community composition in the samples were different for each sampling site. Different layers of the same heterogeneous mat often contained distinct and different communities of cyanobacteria. We observed a relationship between the cyanobacterial community composition and the in situ temperatures of different mat parts. The Greenland mats exhibited a low diversity of anoxygenic phototrophs as compared with other hot spring mats which is possibly related to the photochemical conditions within the mats resulting from the Arctic light regime. SN - 1462-2912 UR - https://www.unboundmedicine.com/medline/citation/17227409/Diversity_of_phototrophic_bacteria_in_microbial_mats_from_Arctic_hot_springs__Greenland__ L2 - https://doi.org/10.1111/j.1462-2920.2006.01103.x DB - PRIME DP - Unbound Medicine ER -