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In vitro cell growth of marine archaeal-bacterial consortia during anaerobic oxidation of methane with sulfate.
Environ Microbiol. 2007 Jan; 9(1):187-96.EM

Abstract

Anoxic sediment from a methane hydrate area (Hydrate Ridge, north-east Pacific; water depth 780 m) was incubated in a long-term laboratory experiment with semi-continuous supply of pressurized [1.4 MPa (14 atm)] methane and sulfate to attempt in vitro propagation of the indigenous consortia of archaea (ANME-2) and bacteria (DSS, Desulfosarcina/Desulfococcus cluster) to which anaerobic oxidation of methane (AOM) with sulfate has been attributed. During 24 months of incubation, the rate of AOM (measured as methane-dependent sulfide formation) increased from 20 to 230 micromol day(-1) (g sediment dry weight)(-1) and the number of aggregates (determined by microscopic counts) from 0.5 x 10(8) to 5.7 x 10(8) (g sediment dry weight)(-1). Fluorescence in situ hybridization targeting 16S rRNA of both partners showed that the newly grown consortia contained central archaeal clusters and peripheral bacterial layers, both with the same morphology and phylogenetic affiliation as in the original sediment. The development of the AOM rate and the total consortia biovolume over time indicated that the consortia grew with a doubling time of approximately 7 months (growth rate 0.003 day(-1)) under the given conditions. The molar growth yield of AOM was approximately 0.6 g cell dry weight (mol CH(4) oxidized)(-1); according to this, only 1% of the consumed methane is channelled into synthesis of consortia biomass. Concentrations of biomarker lipids previously attributed to ANME-2 archaea (e.g. sn-2-hydroxyarchaeol, archaeol, crocetane, pentamethylicosatriene) and Desulfosarcina-like bacteria [e.g. hexadecenoic-11 acid (16:1omega5c), 11,12-methylene-hexadecanoic acid (cy17:0omega5,6)] strongly increased over time (some of them over-proportionally to consortia biovolume), suggesting that they are useful biomarkers to detect active anaerobic methanotrophic consortia in sediments.

Authors+Show Affiliations

Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17227423

Citation

Nauhaus, Katja, et al. "In Vitro Cell Growth of Marine Archaeal-bacterial Consortia During Anaerobic Oxidation of Methane With Sulfate." Environmental Microbiology, vol. 9, no. 1, 2007, pp. 187-96.
Nauhaus K, Albrecht M, Elvert M, et al. In vitro cell growth of marine archaeal-bacterial consortia during anaerobic oxidation of methane with sulfate. Environ Microbiol. 2007;9(1):187-96.
Nauhaus, K., Albrecht, M., Elvert, M., Boetius, A., & Widdel, F. (2007). In vitro cell growth of marine archaeal-bacterial consortia during anaerobic oxidation of methane with sulfate. Environmental Microbiology, 9(1), 187-96.
Nauhaus K, et al. In Vitro Cell Growth of Marine Archaeal-bacterial Consortia During Anaerobic Oxidation of Methane With Sulfate. Environ Microbiol. 2007;9(1):187-96. PubMed PMID: 17227423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro cell growth of marine archaeal-bacterial consortia during anaerobic oxidation of methane with sulfate. AU - Nauhaus,Katja, AU - Albrecht,Melanie, AU - Elvert,Marcus, AU - Boetius,Antje, AU - Widdel,Friedrich, PY - 2007/1/18/pubmed PY - 2007/7/3/medline PY - 2007/1/18/entrez SP - 187 EP - 96 JF - Environmental microbiology JO - Environ. Microbiol. VL - 9 IS - 1 N2 - Anoxic sediment from a methane hydrate area (Hydrate Ridge, north-east Pacific; water depth 780 m) was incubated in a long-term laboratory experiment with semi-continuous supply of pressurized [1.4 MPa (14 atm)] methane and sulfate to attempt in vitro propagation of the indigenous consortia of archaea (ANME-2) and bacteria (DSS, Desulfosarcina/Desulfococcus cluster) to which anaerobic oxidation of methane (AOM) with sulfate has been attributed. During 24 months of incubation, the rate of AOM (measured as methane-dependent sulfide formation) increased from 20 to 230 micromol day(-1) (g sediment dry weight)(-1) and the number of aggregates (determined by microscopic counts) from 0.5 x 10(8) to 5.7 x 10(8) (g sediment dry weight)(-1). Fluorescence in situ hybridization targeting 16S rRNA of both partners showed that the newly grown consortia contained central archaeal clusters and peripheral bacterial layers, both with the same morphology and phylogenetic affiliation as in the original sediment. The development of the AOM rate and the total consortia biovolume over time indicated that the consortia grew with a doubling time of approximately 7 months (growth rate 0.003 day(-1)) under the given conditions. The molar growth yield of AOM was approximately 0.6 g cell dry weight (mol CH(4) oxidized)(-1); according to this, only 1% of the consumed methane is channelled into synthesis of consortia biomass. Concentrations of biomarker lipids previously attributed to ANME-2 archaea (e.g. sn-2-hydroxyarchaeol, archaeol, crocetane, pentamethylicosatriene) and Desulfosarcina-like bacteria [e.g. hexadecenoic-11 acid (16:1omega5c), 11,12-methylene-hexadecanoic acid (cy17:0omega5,6)] strongly increased over time (some of them over-proportionally to consortia biovolume), suggesting that they are useful biomarkers to detect active anaerobic methanotrophic consortia in sediments. SN - 1462-2912 UR - https://www.unboundmedicine.com/medline/citation/17227423/In_vitro_cell_growth_of_marine_archaeal_bacterial_consortia_during_anaerobic_oxidation_of_methane_with_sulfate_ L2 - https://doi.org/10.1111/j.1462-2920.2006.01127.x DB - PRIME DP - Unbound Medicine ER -