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[The experimental research about the effect of Prunella vulgaris L. on Raji cells growth and expression of apoptosis related protein].
Zhong Yao Cai. 2006 Nov; 29(11):1207-10.ZY

Abstract

OBJECTIVE

To investigate the anti-lymphoma effect of Prunella vulgaris L. in order to offer exprimental data for the treatment of lymphoma with Prunella vulgaris L. in clinic.

METHODS

Effect of Prunella vulgaris L. injection on inhibition ratio of cell growth of Raji cells and IC50 were tested by MTT assay. The growth curve line of Raji cells was drawn also by MTT assay. The cellular morphology was observed by invert microscope, Giemas staining and MTT assay. The expression of apoptosis related protein bcl-2, bax was measured by immunocytochemistry and the quantitative analysis was made with figure analysis system.

RESULTS

1. Prunella vulgaris L. could obviously suppress the cell proliferation of Raji cells in a concentration-dependent manner (r = 0.97). The IC50 was 0.118 mg/ml. 2. After Raji cells was reacted with injection of Prunella vulgaris L. (50 mg/ml) , the morphlogical of apoptosis were observed by invert microscope, Giemsa staining and MTT assay.

RESULTS

The results of immunocytochemistry showed that after Raji cells were treated by the injection of Prunella vulgaris L. (50 mg/ml) for 48 hours, the expression of bcl-1 was up-regulated, and the expression of bax was down-regulated. The differences between process group and control group were significant (P < 0.01).

CONCLUSION

Prunella vulgaris L. can suppress the proliferation of Raji cells and may be a new anti-lymphoma drug. Inducing the apoptosis of Raji cells maybe one of anti-lymphoma mechanisms.

Authors+Show Affiliations

Department of Hematology Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. zhangkejie0378@sina.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17228664

Citation

Zhang, Ke-Jie, et al. "[The Experimental Research About the Effect of Prunella Vulgaris L. On Raji Cells Growth and Expression of Apoptosis Related Protein]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 29, no. 11, 2006, pp. 1207-10.
Zhang KJ, Zhang MZ, Wang QD, et al. [The experimental research about the effect of Prunella vulgaris L. on Raji cells growth and expression of apoptosis related protein]. Zhong Yao Cai. 2006;29(11):1207-10.
Zhang, K. J., Zhang, M. Z., Wang, Q. D., & Liu, W. L. (2006). [The experimental research about the effect of Prunella vulgaris L. on Raji cells growth and expression of apoptosis related protein]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 29(11), 1207-10.
Zhang KJ, et al. [The Experimental Research About the Effect of Prunella Vulgaris L. On Raji Cells Growth and Expression of Apoptosis Related Protein]. Zhong Yao Cai. 2006;29(11):1207-10. PubMed PMID: 17228664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The experimental research about the effect of Prunella vulgaris L. on Raji cells growth and expression of apoptosis related protein]. AU - Zhang,Ke-Jie, AU - Zhang,Ming-Zhi, AU - Wang,Qing-Duan, AU - Liu,Wen-Li, PY - 2007/1/19/pubmed PY - 2007/12/6/medline PY - 2007/1/19/entrez SP - 1207 EP - 10 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 29 IS - 11 N2 - OBJECTIVE: To investigate the anti-lymphoma effect of Prunella vulgaris L. in order to offer exprimental data for the treatment of lymphoma with Prunella vulgaris L. in clinic. METHODS: Effect of Prunella vulgaris L. injection on inhibition ratio of cell growth of Raji cells and IC50 were tested by MTT assay. The growth curve line of Raji cells was drawn also by MTT assay. The cellular morphology was observed by invert microscope, Giemas staining and MTT assay. The expression of apoptosis related protein bcl-2, bax was measured by immunocytochemistry and the quantitative analysis was made with figure analysis system. RESULTS: 1. Prunella vulgaris L. could obviously suppress the cell proliferation of Raji cells in a concentration-dependent manner (r = 0.97). The IC50 was 0.118 mg/ml. 2. After Raji cells was reacted with injection of Prunella vulgaris L. (50 mg/ml) , the morphlogical of apoptosis were observed by invert microscope, Giemsa staining and MTT assay. RESULTS: The results of immunocytochemistry showed that after Raji cells were treated by the injection of Prunella vulgaris L. (50 mg/ml) for 48 hours, the expression of bcl-1 was up-regulated, and the expression of bax was down-regulated. The differences between process group and control group were significant (P < 0.01). CONCLUSION: Prunella vulgaris L. can suppress the proliferation of Raji cells and may be a new anti-lymphoma drug. Inducing the apoptosis of Raji cells maybe one of anti-lymphoma mechanisms. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/17228664/[The_experimental_research_about_the_effect_of_Prunella_vulgaris_L__on_Raji_cells_growth_and_expression_of_apoptosis_related_protein]_ DB - PRIME DP - Unbound Medicine ER -