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[Determination of acacetin in Xiangjuganmao Keli (no sweet) by HPLC].
Zhong Yao Cai. 2006 Nov; 29(11):1233-5.ZY

Abstract

OBJECTIVE

To establish a method for the determination of acacetin in Xiangjuganmao Keli (no sweet).

METHODS

Acacetin in powdered herb was extracted by ultrasonator with methanol and was hydrolyzed with hydrochloric acid. Separation was accomplished on an ODS reversed phase column (5 microm, 4.6 x 250 mm) with a mobile phase of methanol-water-acetic acid(350: 150: 2). The detective wavelength was at 340 nm.

RESULTS

The method was accurate, the results were stable and reproducible. The linear range of calibration cure was within the concentration of 2.00 - 10.00 microg/ml (r = 0.9998). The average extraction recovery was 99.9% (n = 6), RSD = 0.41% (n = 6).

CONCLUSION

The method is simple, convenient, sensitive, and reproducible for quality control of Xiangjuganmao Keli (no sweet).

Authors+Show Affiliations

Leiyunshang Medical Co. Ltd., Suzhou 215009, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17228667

Citation

Bian, Jia-Hong, et al. "[Determination of Acacetin in Xiangjuganmao Keli (no Sweet) By HPLC]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 29, no. 11, 2006, pp. 1233-5.
Bian JH, Qian K, Xu X, et al. [Determination of acacetin in Xiangjuganmao Keli (no sweet) by HPLC]. Zhong Yao Cai. 2006;29(11):1233-5.
Bian, J. H., Qian, K., Xu, X., & Shen, J. (2006). [Determination of acacetin in Xiangjuganmao Keli (no sweet) by HPLC]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 29(11), 1233-5.
Bian JH, et al. [Determination of Acacetin in Xiangjuganmao Keli (no Sweet) By HPLC]. Zhong Yao Cai. 2006;29(11):1233-5. PubMed PMID: 17228667.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of acacetin in Xiangjuganmao Keli (no sweet) by HPLC]. AU - Bian,Jia-Hong, AU - Qian,Kun, AU - Xu,Xiang, AU - Shen,Jun, PY - 2007/1/19/pubmed PY - 2007/12/6/medline PY - 2007/1/19/entrez SP - 1233 EP - 5 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 29 IS - 11 N2 - OBJECTIVE: To establish a method for the determination of acacetin in Xiangjuganmao Keli (no sweet). METHODS: Acacetin in powdered herb was extracted by ultrasonator with methanol and was hydrolyzed with hydrochloric acid. Separation was accomplished on an ODS reversed phase column (5 microm, 4.6 x 250 mm) with a mobile phase of methanol-water-acetic acid(350: 150: 2). The detective wavelength was at 340 nm. RESULTS: The method was accurate, the results were stable and reproducible. The linear range of calibration cure was within the concentration of 2.00 - 10.00 microg/ml (r = 0.9998). The average extraction recovery was 99.9% (n = 6), RSD = 0.41% (n = 6). CONCLUSION: The method is simple, convenient, sensitive, and reproducible for quality control of Xiangjuganmao Keli (no sweet). SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/17228667/[Determination_of_acacetin_in_Xiangjuganmao_Keli__no_sweet__by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -