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Ethyl acetate extract from Angelica Dahuricae Radix inhibits lipopolysaccharide-induced production of nitric oxide, prostaglandin E2 and tumor necrosis factor-alphavia mitogen-activated protein kinases and nuclear factor-kappaB in macrophages.
Pharmacol Res 2007; 55(4):263-70PR

Abstract

Angelica dahurica (Umbelliferae) has been used to treat headache of common cold, supraorbital neuralgia, painful swelling on the body, nasal stuffiness, leukorrhea and arthralgia due to wind-dampness in Korean traditional medicine. It is also claimed to be effective in the treatment of acne, erythema, headache, toothache, sinusitis, colds and flu. The present study focused whether the ethyl acetate extract from Angelica Dahuricae Radix (EAAD) inhibits production of nitric oxide (NO), prostaglandin E(2) (PGE(2)) and tumor necrosis factor (TNF)-alpha, as well as expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappaB (NF-kappaB) and mitogen-activated protein kinases (MAPKs) in lipopolysaccharide (LPS)-stimulated macrophages. EAAD inhibited LPS-induced NO, PGE(2) and TNF-alpha production as well as expression of iNOS and COX-2 in RAW 264.7 cells. EAAD inhibited LPS-induced TNF-alpha production in THP-1 cells. Furthermore, EAAD suppressed LPS-induced phosphorylation of p38 MAPK and extracellular-signal regulated kinases 1/2 (ERK1/2), I-kappaBalpha degradation, and NF-kappaB activation in RAW 264.7 cells. These results suggest that EAAD has the inhibitory effects on LPS-induced TNF-alpha, NO and PGE(2) production, and expression of iNOS and COX-2 in macrophage through blockade in the phosphorylation of MAPKs, following I-kappaBalpha degradation and NF-kappaB activation.

Authors+Show Affiliations

Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17229575

Citation

Kang, Ok-Hwa, et al. "Ethyl Acetate Extract From Angelica Dahuricae Radix Inhibits Lipopolysaccharide-induced Production of Nitric Oxide, Prostaglandin E2 and Tumor Necrosis Factor-alphavia Mitogen-activated Protein Kinases and Nuclear factor-kappaB in Macrophages." Pharmacological Research, vol. 55, no. 4, 2007, pp. 263-70.
Kang OH, Lee GH, Choi HJ, et al. Ethyl acetate extract from Angelica Dahuricae Radix inhibits lipopolysaccharide-induced production of nitric oxide, prostaglandin E2 and tumor necrosis factor-alphavia mitogen-activated protein kinases and nuclear factor-kappaB in macrophages. Pharmacol Res. 2007;55(4):263-70.
Kang, O. H., Lee, G. H., Choi, H. J., Park, P. S., Chae, H. S., Jeong, S. I., ... Kwon, D. Y. (2007). Ethyl acetate extract from Angelica Dahuricae Radix inhibits lipopolysaccharide-induced production of nitric oxide, prostaglandin E2 and tumor necrosis factor-alphavia mitogen-activated protein kinases and nuclear factor-kappaB in macrophages. Pharmacological Research, 55(4), pp. 263-70.
Kang OH, et al. Ethyl Acetate Extract From Angelica Dahuricae Radix Inhibits Lipopolysaccharide-induced Production of Nitric Oxide, Prostaglandin E2 and Tumor Necrosis Factor-alphavia Mitogen-activated Protein Kinases and Nuclear factor-kappaB in Macrophages. Pharmacol Res. 2007;55(4):263-70. PubMed PMID: 17229575.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethyl acetate extract from Angelica Dahuricae Radix inhibits lipopolysaccharide-induced production of nitric oxide, prostaglandin E2 and tumor necrosis factor-alphavia mitogen-activated protein kinases and nuclear factor-kappaB in macrophages. AU - Kang,Ok-Hwa, AU - Lee,Go-Hoon, AU - Choi,Hyuk Joon, AU - Park,Pil Sang, AU - Chae,Hee-Sung, AU - Jeong,Seung-Il, AU - Kim,Youn-Chul, AU - Sohn,Dong Hwan, AU - Park,Hyun, AU - Lee,John Hwa, AU - Kwon,Dong-Yeul, Y1 - 2006/12/19/ PY - 2006/07/05/received PY - 2006/11/30/revised PY - 2006/12/07/accepted PY - 2007/1/19/pubmed PY - 2007/8/30/medline PY - 2007/1/19/entrez SP - 263 EP - 70 JF - Pharmacological research JO - Pharmacol. Res. VL - 55 IS - 4 N2 - Angelica dahurica (Umbelliferae) has been used to treat headache of common cold, supraorbital neuralgia, painful swelling on the body, nasal stuffiness, leukorrhea and arthralgia due to wind-dampness in Korean traditional medicine. It is also claimed to be effective in the treatment of acne, erythema, headache, toothache, sinusitis, colds and flu. The present study focused whether the ethyl acetate extract from Angelica Dahuricae Radix (EAAD) inhibits production of nitric oxide (NO), prostaglandin E(2) (PGE(2)) and tumor necrosis factor (TNF)-alpha, as well as expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappaB (NF-kappaB) and mitogen-activated protein kinases (MAPKs) in lipopolysaccharide (LPS)-stimulated macrophages. EAAD inhibited LPS-induced NO, PGE(2) and TNF-alpha production as well as expression of iNOS and COX-2 in RAW 264.7 cells. EAAD inhibited LPS-induced TNF-alpha production in THP-1 cells. Furthermore, EAAD suppressed LPS-induced phosphorylation of p38 MAPK and extracellular-signal regulated kinases 1/2 (ERK1/2), I-kappaBalpha degradation, and NF-kappaB activation in RAW 264.7 cells. These results suggest that EAAD has the inhibitory effects on LPS-induced TNF-alpha, NO and PGE(2) production, and expression of iNOS and COX-2 in macrophage through blockade in the phosphorylation of MAPKs, following I-kappaBalpha degradation and NF-kappaB activation. SN - 1043-6618 UR - https://www.unboundmedicine.com/medline/citation/17229575/Ethyl_acetate_extract_from_Angelica_Dahuricae_Radix_inhibits_lipopolysaccharide_induced_production_of_nitric_oxide_prostaglandin_E2_and_tumor_necrosis_factor_alphavia_mitogen_activated_protein_kinases_and_nuclear_factor_kappaB_in_macrophages_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-6618(06)00214-3 DB - PRIME DP - Unbound Medicine ER -