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Accuracy of HPV testing of vaginal smear obtained with a novel self-sampling device.
Acta Obstet Gynecol Scand. 2007; 86(1):16-21.AO

Abstract

BACKGROUND

Most of women diagnosed as having cervical cancer have not participated in organized cytological screening. Aim. A study was conducted to evaluate the accuracy of human papilloma virus testing by self-collected vaginal samples in comparison to regular cytological screening. The agreement of hybrid capture 2 assay and polymerase chain reaction assay for detection of human papilloma virus DNA in self-collected vaginal samples and clinician-obtained cervical smears was investigated.

METHOD

Forty-three women aged 23-58 years admitted for further examination due to previous positive cytology in the organized screening participated in self-collecting of vaginal samples with a novel self-sampling device. During the visit a clinician also collected a cervical smear using a cytobrush. The vaginal samples collected with the self-sampling device were analyzed for high-risk human papilloma virus with the hybrid capture 2 assay technique and the cervical smears were Pap-stained, examined cytologically and after that reanalyzed for human papilloma virus DNA using a polymerase chain reaction assay.

RESULT

The vaginal samples were positive for high-risk human papilloma virus in 37% of the cases using hybrid capture 2 assay. Twelve of the 43 Pap smears showed positive cytology (ASCUS-CIN 3), of which 4 showed CIN 2-3. When polymerase chain reaction assay was performed, human papilloma virus DNA was detected in 40% of the glass slides. The agreement between cytology and the two human papilloma virus testing techniques was 67-74% (kappa 0.27-0.45) and the agreement between the two human papilloma virus tests was 70% (kappa 0.36).

CONCLUSION

Testing for high-risk human papilloma virus can identify more women at risk of developing cervical cancer than cytology irrespective of the sampling method. Furthermore, offering a self-sampling device for collection of vaginal smear seems to be a useful screening tool for cervical cancer among women not responding to an invitation for smear sampling.

Authors+Show Affiliations

Department of Genetics and Pathology, University Hospital of Uppsala, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17230283

Citation

Stenvall, Harriet, et al. "Accuracy of HPV Testing of Vaginal Smear Obtained With a Novel Self-sampling Device." Acta Obstetricia Et Gynecologica Scandinavica, vol. 86, no. 1, 2007, pp. 16-21.
Stenvall H, Wikström I, Backlund I, et al. Accuracy of HPV testing of vaginal smear obtained with a novel self-sampling device. Acta Obstet Gynecol Scand. 2007;86(1):16-21.
Stenvall, H., Wikström, I., Backlund, I., & Wilander, E. (2007). Accuracy of HPV testing of vaginal smear obtained with a novel self-sampling device. Acta Obstetricia Et Gynecologica Scandinavica, 86(1), 16-21.
Stenvall H, et al. Accuracy of HPV Testing of Vaginal Smear Obtained With a Novel Self-sampling Device. Acta Obstet Gynecol Scand. 2007;86(1):16-21. PubMed PMID: 17230283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accuracy of HPV testing of vaginal smear obtained with a novel self-sampling device. AU - Stenvall,Harriet, AU - Wikström,Ingrid, AU - Backlund,Ingrid, AU - Wilander,Erik, PY - 2007/1/19/pubmed PY - 2007/2/16/medline PY - 2007/1/19/entrez SP - 16 EP - 21 JF - Acta obstetricia et gynecologica Scandinavica JO - Acta Obstet Gynecol Scand VL - 86 IS - 1 N2 - BACKGROUND: Most of women diagnosed as having cervical cancer have not participated in organized cytological screening. Aim. A study was conducted to evaluate the accuracy of human papilloma virus testing by self-collected vaginal samples in comparison to regular cytological screening. The agreement of hybrid capture 2 assay and polymerase chain reaction assay for detection of human papilloma virus DNA in self-collected vaginal samples and clinician-obtained cervical smears was investigated. METHOD: Forty-three women aged 23-58 years admitted for further examination due to previous positive cytology in the organized screening participated in self-collecting of vaginal samples with a novel self-sampling device. During the visit a clinician also collected a cervical smear using a cytobrush. The vaginal samples collected with the self-sampling device were analyzed for high-risk human papilloma virus with the hybrid capture 2 assay technique and the cervical smears were Pap-stained, examined cytologically and after that reanalyzed for human papilloma virus DNA using a polymerase chain reaction assay. RESULT: The vaginal samples were positive for high-risk human papilloma virus in 37% of the cases using hybrid capture 2 assay. Twelve of the 43 Pap smears showed positive cytology (ASCUS-CIN 3), of which 4 showed CIN 2-3. When polymerase chain reaction assay was performed, human papilloma virus DNA was detected in 40% of the glass slides. The agreement between cytology and the two human papilloma virus testing techniques was 67-74% (kappa 0.27-0.45) and the agreement between the two human papilloma virus tests was 70% (kappa 0.36). CONCLUSION: Testing for high-risk human papilloma virus can identify more women at risk of developing cervical cancer than cytology irrespective of the sampling method. Furthermore, offering a self-sampling device for collection of vaginal smear seems to be a useful screening tool for cervical cancer among women not responding to an invitation for smear sampling. SN - 0001-6349 UR - https://www.unboundmedicine.com/medline/citation/17230283/Accuracy_of_HPV_testing_of_vaginal_smear_obtained_with_a_novel_self_sampling_device_ L2 - https://doi.org/10.1080/00016340601033667 DB - PRIME DP - Unbound Medicine ER -