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Controlling the chromatin organization of vitamin D target genes by multiple vitamin D receptor binding sites.
J Steroid Biochem Mol Biol 2007; 103(3-5):338-43JS

Abstract

An essential prerequisite for the direct modulation of transcription by 1alpha,25-dihydroxy vitamin D(3) (1alpha,25(OH)(2)D(3)) is the location of at least one activated vitamin D receptor (VDR) protein close to the transcription start site of the respective primary 1alpha,25(OH)(2)D(3) target gene. This is achieved through the specific binding of VDR to a 1alpha,25(OH)(2)D(3) response element (VDRE). Although these elements are well characterized in vitro, the function of VDREs in living cells in the context of chromatin is still largely unknown. To resolve this issue, approximately 8kB of the promoter regions of the primary 1alpha,25(OH)(2)D(3) target genes CYP24, cyclin C and p21((Waf1/Cip1)) were screened by chromatin immunoprecipitation (ChIP) assays for VDR binding sites using antibodies against VDR and its partner proteins. This approach identified three to four functional VDREs per gene promoter. In parallel, in silico screening of the extended gene areas (i.e. 10kB of promoter, introns, exons and 10kB of the downstream region) of all six members of the insulin-like growth factor binding protein (IGFBP) gene family was performed. Gel shift, reporter gene and ChIP assays identified in total 10 functional VDREs in the genes IGFBP1, IGFBP3 and IGFBP5. Taken together, both screening approaches suggest that a reasonable proportion of all VDR target genes, if not all, are under the control of multiple VDREs.

Authors+Show Affiliations

Department of Biochemistry, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland. carlberg@messi.uku.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17234401

Citation

Carlberg, Carsten, et al. "Controlling the Chromatin Organization of Vitamin D Target Genes By Multiple Vitamin D Receptor Binding Sites." The Journal of Steroid Biochemistry and Molecular Biology, vol. 103, no. 3-5, 2007, pp. 338-43.
Carlberg C, Dunlop TW, Saramäki A, et al. Controlling the chromatin organization of vitamin D target genes by multiple vitamin D receptor binding sites. J Steroid Biochem Mol Biol. 2007;103(3-5):338-43.
Carlberg, C., Dunlop, T. W., Saramäki, A., Sinkkonen, L., Matilainen, M., & Väisänen, S. (2007). Controlling the chromatin organization of vitamin D target genes by multiple vitamin D receptor binding sites. The Journal of Steroid Biochemistry and Molecular Biology, 103(3-5), pp. 338-43.
Carlberg C, et al. Controlling the Chromatin Organization of Vitamin D Target Genes By Multiple Vitamin D Receptor Binding Sites. J Steroid Biochem Mol Biol. 2007;103(3-5):338-43. PubMed PMID: 17234401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Controlling the chromatin organization of vitamin D target genes by multiple vitamin D receptor binding sites. AU - Carlberg,Carsten, AU - Dunlop,Thomas W, AU - Saramäki,Anna, AU - Sinkkonen,Lasse, AU - Matilainen,Merja, AU - Väisänen,Sami, Y1 - 2007/01/16/ PY - 2006/11/30/received PY - 2007/1/20/pubmed PY - 2007/5/12/medline PY - 2007/1/20/entrez SP - 338 EP - 43 JF - The Journal of steroid biochemistry and molecular biology JO - J. Steroid Biochem. Mol. Biol. VL - 103 IS - 3-5 N2 - An essential prerequisite for the direct modulation of transcription by 1alpha,25-dihydroxy vitamin D(3) (1alpha,25(OH)(2)D(3)) is the location of at least one activated vitamin D receptor (VDR) protein close to the transcription start site of the respective primary 1alpha,25(OH)(2)D(3) target gene. This is achieved through the specific binding of VDR to a 1alpha,25(OH)(2)D(3) response element (VDRE). Although these elements are well characterized in vitro, the function of VDREs in living cells in the context of chromatin is still largely unknown. To resolve this issue, approximately 8kB of the promoter regions of the primary 1alpha,25(OH)(2)D(3) target genes CYP24, cyclin C and p21((Waf1/Cip1)) were screened by chromatin immunoprecipitation (ChIP) assays for VDR binding sites using antibodies against VDR and its partner proteins. This approach identified three to four functional VDREs per gene promoter. In parallel, in silico screening of the extended gene areas (i.e. 10kB of promoter, introns, exons and 10kB of the downstream region) of all six members of the insulin-like growth factor binding protein (IGFBP) gene family was performed. Gel shift, reporter gene and ChIP assays identified in total 10 functional VDREs in the genes IGFBP1, IGFBP3 and IGFBP5. Taken together, both screening approaches suggest that a reasonable proportion of all VDR target genes, if not all, are under the control of multiple VDREs. SN - 0960-0760 UR - https://www.unboundmedicine.com/medline/citation/17234401/Controlling_the_chromatin_organization_of_vitamin_D_target_genes_by_multiple_vitamin_D_receptor_binding_sites_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-0760(06)00421-3 DB - PRIME DP - Unbound Medicine ER -