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Effects of omega-3 fatty acids on leukocyte Th1/Th2 cytokine and integrin expression in rats with gut-derived sepsis.
Nutrition. 2007 Feb; 23(2):179-86.N

Abstract

OBJECTIVES

This study examined the effect of fish oil (FO)-enriched diets before and/or omega-3 fatty acid-containing total parenteral nutrition (TPN) after sepsis on the distribution of the T-lymphocyte subpopulation, intracellular cytokine, and intestinal immunity in rats with gut-derived sepsis.

METHODS

Rats were assigned to a control or one of four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet, and groups 3 and 4 received FO instead of 20% soybean oil. After feeding the diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP) in the experimental groups, whereas a sham operation was performed in the control group. TPN was maintained for 3 d after the CLP or sham operation. The control group and groups 1 and 3 were infused with conventional TPN, whereas the TPN solution used for groups 2 and 4 were supplemented with FO. All rats were sacrificed 3 d after the operation to examine their immune responses.

RESULTS

Plasma and intestinal immunoglobin A levels were higher in the FO-supplemented groups than in the control group and group 1. Lymphocyte interferon-gamma expression in groups 3 and 4 was significantly lower, whereas interleukin-4 expression was higher than those of the control group and groups 1 and 2. The splenocyte CD4 percentage in groups 3 and 4 and the CD4/CD8 ratio in group 4 were significantly higher than those in group 1.

CONCLUSION

These findings suggest that FO administration before and/or after CLP are not immunosuppressive. FO-enriched diets before or before and after CLP resulted in a T-helper type 2 response and enhanced immunoglobulin A secretion. In addition, the splenocyte CD4 levels and CD4/CD8 ratio were maintained in rats with gut-derived sepsis.

Authors+Show Affiliations

Department of Primary Care Medicine, National Taiwan University Hospital and National Taiwan University, College of Medicine, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17234509

Citation

Lin, Ming-Tsan, et al. "Effects of Omega-3 Fatty Acids On Leukocyte Th1/Th2 Cytokine and Integrin Expression in Rats With Gut-derived Sepsis." Nutrition (Burbank, Los Angeles County, Calif.), vol. 23, no. 2, 2007, pp. 179-86.
Lin MT, Hsu CS, Yeh SL, et al. Effects of omega-3 fatty acids on leukocyte Th1/Th2 cytokine and integrin expression in rats with gut-derived sepsis. Nutrition. 2007;23(2):179-86.
Lin, M. T., Hsu, C. S., Yeh, S. L., Yeh, C. L., Chang, K. J., Lee, P. H., & Chen, W. J. (2007). Effects of omega-3 fatty acids on leukocyte Th1/Th2 cytokine and integrin expression in rats with gut-derived sepsis. Nutrition (Burbank, Los Angeles County, Calif.), 23(2), 179-86.
Lin MT, et al. Effects of Omega-3 Fatty Acids On Leukocyte Th1/Th2 Cytokine and Integrin Expression in Rats With Gut-derived Sepsis. Nutrition. 2007;23(2):179-86. PubMed PMID: 17234509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of omega-3 fatty acids on leukocyte Th1/Th2 cytokine and integrin expression in rats with gut-derived sepsis. AU - Lin,Ming-Tsan, AU - Hsu,Chun-Sen, AU - Yeh,Sung-Ling, AU - Yeh,Chiu-Li, AU - Chang,King-Jen, AU - Lee,Po-Huang, AU - Chen,Wei-Jao, PY - 2006/07/20/received PY - 2006/10/20/revised PY - 2006/11/09/accepted PY - 2007/1/20/pubmed PY - 2007/4/18/medline PY - 2007/1/20/entrez SP - 179 EP - 86 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 23 IS - 2 N2 - OBJECTIVES: This study examined the effect of fish oil (FO)-enriched diets before and/or omega-3 fatty acid-containing total parenteral nutrition (TPN) after sepsis on the distribution of the T-lymphocyte subpopulation, intracellular cytokine, and intestinal immunity in rats with gut-derived sepsis. METHODS: Rats were assigned to a control or one of four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet, and groups 3 and 4 received FO instead of 20% soybean oil. After feeding the diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP) in the experimental groups, whereas a sham operation was performed in the control group. TPN was maintained for 3 d after the CLP or sham operation. The control group and groups 1 and 3 were infused with conventional TPN, whereas the TPN solution used for groups 2 and 4 were supplemented with FO. All rats were sacrificed 3 d after the operation to examine their immune responses. RESULTS: Plasma and intestinal immunoglobin A levels were higher in the FO-supplemented groups than in the control group and group 1. Lymphocyte interferon-gamma expression in groups 3 and 4 was significantly lower, whereas interleukin-4 expression was higher than those of the control group and groups 1 and 2. The splenocyte CD4 percentage in groups 3 and 4 and the CD4/CD8 ratio in group 4 were significantly higher than those in group 1. CONCLUSION: These findings suggest that FO administration before and/or after CLP are not immunosuppressive. FO-enriched diets before or before and after CLP resulted in a T-helper type 2 response and enhanced immunoglobulin A secretion. In addition, the splenocyte CD4 levels and CD4/CD8 ratio were maintained in rats with gut-derived sepsis. SN - 0899-9007 UR - https://www.unboundmedicine.com/medline/citation/17234509/Effects_of_omega_3_fatty_acids_on_leukocyte_Th1/Th2_cytokine_and_integrin_expression_in_rats_with_gut_derived_sepsis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(06)00396-0 DB - PRIME DP - Unbound Medicine ER -