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Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.
J Dairy Sci 2007; 90(2):918-27JD

Abstract

To elucidate the possible role of selenoproteins for milk formation and mammary gland physiology, the activities of selenoprotein enzymes and the expression of selenoprotein genes were studied in the bovine mammary gland. Messenger RNA was demonstrated for selenoprotein P, thioredoxin reductase 1, and for glutathione peroxidase (GPx) 1, 3, and 4. Significant differences in mRNA expression between the cows were seen for GPx 1 and GPx 3. The enzyme activity of glutathione peroxidase varied approximately 16-fold among cows, and the activity of thioredoxin reductase and the concentration of soluble Se varied approximately 6-fold among cows. There were positive correlations between glutathione peroxidase activity, thioredoxin reductase activity, and soluble Se, the correlation between glutathione peroxidase activity and soluble Se being the strongest. Furthermore, selenoprotein P expression correlated with GPx 1 mRNA expression and with soluble Se. There was also a correlation between glutathione peroxidase activity and the mRNA expression of GPx 1. The general conclusion from the data was that the activity of glutathione peroxidase and thioredoxin reductase and the mRNA expression of selenoprotein P and GPx 1 and 3 were influenced by Se status, but the expression of GPx 4 and thioredoxin reductase 1 were not. These results indicate that the Se status in mammary tissue is an important regulator of selenoprotein activity and expression, but that other factors are also in operation.

Authors+Show Affiliations

Biomedical Nutrition, Faculty of Engineering LTH, Lund University, Lund, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17235168

Citation

Bruzelius, K, et al. "Occurrence of Selenoprotein Enzyme Activities and mRNA in Bovine Mammary Tissue." Journal of Dairy Science, vol. 90, no. 2, 2007, pp. 918-27.
Bruzelius K, Hoac T, Sundler R, et al. Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue. J Dairy Sci. 2007;90(2):918-27.
Bruzelius, K., Hoac, T., Sundler, R., Onning, G., & Akesson, B. (2007). Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue. Journal of Dairy Science, 90(2), pp. 918-27.
Bruzelius K, et al. Occurrence of Selenoprotein Enzyme Activities and mRNA in Bovine Mammary Tissue. J Dairy Sci. 2007;90(2):918-27. PubMed PMID: 17235168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue. AU - Bruzelius,K, AU - Hoac,T, AU - Sundler,R, AU - Onning,G, AU - Akesson,B, PY - 2007/1/20/pubmed PY - 2007/5/4/medline PY - 2007/1/20/entrez SP - 918 EP - 27 JF - Journal of dairy science JO - J. Dairy Sci. VL - 90 IS - 2 N2 - To elucidate the possible role of selenoproteins for milk formation and mammary gland physiology, the activities of selenoprotein enzymes and the expression of selenoprotein genes were studied in the bovine mammary gland. Messenger RNA was demonstrated for selenoprotein P, thioredoxin reductase 1, and for glutathione peroxidase (GPx) 1, 3, and 4. Significant differences in mRNA expression between the cows were seen for GPx 1 and GPx 3. The enzyme activity of glutathione peroxidase varied approximately 16-fold among cows, and the activity of thioredoxin reductase and the concentration of soluble Se varied approximately 6-fold among cows. There were positive correlations between glutathione peroxidase activity, thioredoxin reductase activity, and soluble Se, the correlation between glutathione peroxidase activity and soluble Se being the strongest. Furthermore, selenoprotein P expression correlated with GPx 1 mRNA expression and with soluble Se. There was also a correlation between glutathione peroxidase activity and the mRNA expression of GPx 1. The general conclusion from the data was that the activity of glutathione peroxidase and thioredoxin reductase and the mRNA expression of selenoprotein P and GPx 1 and 3 were influenced by Se status, but the expression of GPx 4 and thioredoxin reductase 1 were not. These results indicate that the Se status in mammary tissue is an important regulator of selenoprotein activity and expression, but that other factors are also in operation. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/17235168/Occurrence_of_selenoprotein_enzyme_activities_and_mRNA_in_bovine_mammary_tissue_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(07)71575-8 DB - PRIME DP - Unbound Medicine ER -