Tags

Type your tag names separated by a space and hit enter

Short-term electrical stimulation alters tongue muscle fibre type composition.
Arch Oral Biol. 2007 Jun; 52(6):544-51.AO

Abstract

OBJECTIVE

To examine whether short-term exogenous activation of a tongue muscle induced a phenotypic shift from a fast to a slow fibre-type, and thus assess a potential therapeutic avenue to protect against obstructive sleep apnoea (OSA).

METHODS

New Zealand White rabbit genioglossus (GG) muscle, characteristically a fast muscle, was continuously stimulated at a frequency attributed to slow muscle (10Hz, 3V DC pulses) using an implanted micro-circuit for 7 days. Changes in muscle fibre types and aerobic capacity were assessed between stimulated and un-stimulated (control) groups using immunohistochemistry and electrophoresis for myosin heavy chain (MHC) and assayed for citrate synthase.

RESULTS

Compared to the un-stimulated control group, stimulated GG muscles had more (approximately 13%) type I MHC (slow-twitch) content; a proportional decrease in type II MHC (fast-twitch) isoform also occurred in the stimulated GG muscle (P<0.05). Electrophoresis analysis on whole muscle and single fibre MHC showed an increased type I expression in the stimulated GG muscle (P<0.01). A commensurate rise in citrate synthase activity, indicating a change in aerobic capacity, was also observed in the stimulated GG muscles.

CONCLUSION

Together, these results demonstrate a successful alteration in tongue muscle characteristics using exogenous electrical stimulation and perhaps a potential therapeutic application for OSA.

Authors+Show Affiliations

UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA. epae@dent.ucla.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17239813

Citation

Pae, Eung-Kwon, et al. "Short-term Electrical Stimulation Alters Tongue Muscle Fibre Type Composition." Archives of Oral Biology, vol. 52, no. 6, 2007, pp. 544-51.
Pae EK, Hyatt JP, Wu J, et al. Short-term electrical stimulation alters tongue muscle fibre type composition. Arch Oral Biol. 2007;52(6):544-51.
Pae, E. K., Hyatt, J. P., Wu, J., & Chien, P. (2007). Short-term electrical stimulation alters tongue muscle fibre type composition. Archives of Oral Biology, 52(6), 544-51.
Pae EK, et al. Short-term Electrical Stimulation Alters Tongue Muscle Fibre Type Composition. Arch Oral Biol. 2007;52(6):544-51. PubMed PMID: 17239813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Short-term electrical stimulation alters tongue muscle fibre type composition. AU - Pae,Eung-Kwon, AU - Hyatt,Jon-Philippe K, AU - Wu,Jennifer, AU - Chien,Patricia, Y1 - 2007/01/19/ PY - 2006/05/11/received PY - 2006/12/01/revised PY - 2006/12/05/accepted PY - 2007/1/24/pubmed PY - 2008/1/3/medline PY - 2007/1/24/entrez SP - 544 EP - 51 JF - Archives of oral biology JO - Arch. Oral Biol. VL - 52 IS - 6 N2 - OBJECTIVE: To examine whether short-term exogenous activation of a tongue muscle induced a phenotypic shift from a fast to a slow fibre-type, and thus assess a potential therapeutic avenue to protect against obstructive sleep apnoea (OSA). METHODS: New Zealand White rabbit genioglossus (GG) muscle, characteristically a fast muscle, was continuously stimulated at a frequency attributed to slow muscle (10Hz, 3V DC pulses) using an implanted micro-circuit for 7 days. Changes in muscle fibre types and aerobic capacity were assessed between stimulated and un-stimulated (control) groups using immunohistochemistry and electrophoresis for myosin heavy chain (MHC) and assayed for citrate synthase. RESULTS: Compared to the un-stimulated control group, stimulated GG muscles had more (approximately 13%) type I MHC (slow-twitch) content; a proportional decrease in type II MHC (fast-twitch) isoform also occurred in the stimulated GG muscle (P<0.05). Electrophoresis analysis on whole muscle and single fibre MHC showed an increased type I expression in the stimulated GG muscle (P<0.01). A commensurate rise in citrate synthase activity, indicating a change in aerobic capacity, was also observed in the stimulated GG muscles. CONCLUSION: Together, these results demonstrate a successful alteration in tongue muscle characteristics using exogenous electrical stimulation and perhaps a potential therapeutic application for OSA. SN - 0003-9969 UR - https://www.unboundmedicine.com/medline/citation/17239813/Short_term_electrical_stimulation_alters_tongue_muscle_fibre_type_composition_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(06)00352-9 DB - PRIME DP - Unbound Medicine ER -