Tags

Type your tag names separated by a space and hit enter

Multiplexed quantification of dementia biomarkers in the CSF of patients with early dementias and MCI: a multicenter study.
Neurobiol Aging 2008; 29(6):812-8NA

Abstract

In this report we evaluated the clinical performance of APOE genotyping and three protein biomarkers (total tau, beta-amyloid(1-42), and tau phosphorylated at threonine 181) in a prospective multicenter study using the INNO-BIA AlzBio3 assay applied on Luminex platform. Concentration of biomarkers of Alzheimer's disease in cerebrospinal fluid (CSF) was measured with multiplexing technology (n=223), and compared to the results of ELISA assays in patients with early dementias or mild cognitive impairment (MCI) collected at 12 gerontopsychiatric university departments, and APOE genotyping was performed. Concentrations of Abeta(1-42) were statistically significantly lower in MCI-AD subjects compared to MCI-O, and significantly lower in D-AD patients compared to MCI-O. P-tau(181P) concentrations were significantly higher in MCI-AD patients compared to MCI-O, and significantly higher in D-AD patients compared to MCI-O. The total tau concentrations in MCI-AD patients were significantly higher compared to MCI-O, and higher in D-AD compared to MCI-O, moreover, the concentration of total tau was significantly higher in D-AD compared to MCI-AD patients. For the differential diagnosis between D-AD and D-O, the optimal cutoff concentration of Abeta(1-42) was 197.7 pg/mL, and that for P-tau(181P) was 47.9 pg/mL. These cutoff values were also applied to discriminate between MCI-AD and MCI-O subjects. Simultaneous measurement of the biomarkers significantly improves management of the samples and quality control of the assays' performance.

Authors+Show Affiliations

Department of Psychiatry and Psychotherapy, University of Erlangen-Nuremberg, Erlangen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17239996

Citation

Lewczuk, Piotr, et al. "Multiplexed Quantification of Dementia Biomarkers in the CSF of Patients With Early Dementias and MCI: a Multicenter Study." Neurobiology of Aging, vol. 29, no. 6, 2008, pp. 812-8.
Lewczuk P, Kornhuber J, Vanderstichele H, et al. Multiplexed quantification of dementia biomarkers in the CSF of patients with early dementias and MCI: a multicenter study. Neurobiol Aging. 2008;29(6):812-8.
Lewczuk, P., Kornhuber, J., Vanderstichele, H., Vanmechelen, E., Esselmann, H., Bibl, M., ... Wiltfang, J. (2008). Multiplexed quantification of dementia biomarkers in the CSF of patients with early dementias and MCI: a multicenter study. Neurobiology of Aging, 29(6), pp. 812-8.
Lewczuk P, et al. Multiplexed Quantification of Dementia Biomarkers in the CSF of Patients With Early Dementias and MCI: a Multicenter Study. Neurobiol Aging. 2008;29(6):812-8. PubMed PMID: 17239996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplexed quantification of dementia biomarkers in the CSF of patients with early dementias and MCI: a multicenter study. AU - Lewczuk,Piotr, AU - Kornhuber,Johannes, AU - Vanderstichele,Hugo, AU - Vanmechelen,Eugeen, AU - Esselmann,Hermann, AU - Bibl,Mirko, AU - Wolf,Stefanie, AU - Otto,Markus, AU - Reulbach,Udo, AU - Kölsch,Heike, AU - Jessen,Frank, AU - Schröder,Johannes, AU - Schönknecht,Peter, AU - Hampel,Harald, AU - Peters,Oliver, AU - Weimer,Erik, AU - Perneczky,Robert, AU - Jahn,Holger, AU - Luckhaus,Christian, AU - Lamla,Ulrich, AU - Supprian,Tillmann, AU - Maler,Juan Manuel, AU - Wiltfang,Jens, Y1 - 2007/01/19/ PY - 2006/07/13/received PY - 2006/12/15/revised PY - 2006/12/17/accepted PY - 2007/1/24/pubmed PY - 2008/6/21/medline PY - 2007/1/24/entrez SP - 812 EP - 8 JF - Neurobiology of aging JO - Neurobiol. Aging VL - 29 IS - 6 N2 - In this report we evaluated the clinical performance of APOE genotyping and three protein biomarkers (total tau, beta-amyloid(1-42), and tau phosphorylated at threonine 181) in a prospective multicenter study using the INNO-BIA AlzBio3 assay applied on Luminex platform. Concentration of biomarkers of Alzheimer's disease in cerebrospinal fluid (CSF) was measured with multiplexing technology (n=223), and compared to the results of ELISA assays in patients with early dementias or mild cognitive impairment (MCI) collected at 12 gerontopsychiatric university departments, and APOE genotyping was performed. Concentrations of Abeta(1-42) were statistically significantly lower in MCI-AD subjects compared to MCI-O, and significantly lower in D-AD patients compared to MCI-O. P-tau(181P) concentrations were significantly higher in MCI-AD patients compared to MCI-O, and significantly higher in D-AD patients compared to MCI-O. The total tau concentrations in MCI-AD patients were significantly higher compared to MCI-O, and higher in D-AD compared to MCI-O, moreover, the concentration of total tau was significantly higher in D-AD compared to MCI-AD patients. For the differential diagnosis between D-AD and D-O, the optimal cutoff concentration of Abeta(1-42) was 197.7 pg/mL, and that for P-tau(181P) was 47.9 pg/mL. These cutoff values were also applied to discriminate between MCI-AD and MCI-O subjects. Simultaneous measurement of the biomarkers significantly improves management of the samples and quality control of the assays' performance. SN - 1558-1497 UR - https://www.unboundmedicine.com/medline/citation/17239996/Multiplexed_quantification_of_dementia_biomarkers_in_the_CSF_of_patients_with_early_dementias_and_MCI:_a_multicenter_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0197-4580(06)00469-6 DB - PRIME DP - Unbound Medicine ER -