Tags

Type your tag names separated by a space and hit enter

The influence of alpha1-adrenoreceptors on neuropeptide release from primary sensory neurons of the lower urinary tract.
Eur Urol. 2007 Sep; 52(3):901-8.EU

Abstract

OBJECTIVES

Adrenergic alpha(1)-receptors agonists and antagonists have been reported to increase and reduce, respectively, neurogenic inflammatory responses mediated by capsaicin-sensitive sensory neurons. However, the precise role and localization of the alpha(1)-adrenoceptors involved in these effects are not known.

METHODS

We have studied in the rat whether functional alpha(1)-adrenoreceptors are expressed in primary sensory neurons, and whether they regulate neurogenic inflammation and nociceptive responses in the urinary bladder.

RESULTS

The alpha(1)-adrenoreceptor agonist phenylephrine (1 micromol/l) (1) mobilized intracellular Ca(2+) in cultured lumbar and sacral dorsal root ganglia neurons, (2) caused the release of substance P (SP) from terminals of capsaicin-sensitive sensory neurons from the lumbar enlargement of the dorsal spinal cord and urinary bladder, and (3) increased plasma protein extravasation in the urinary bladder. All these effects were abolished by the alpha(1)-adrenoceptor antagonist alfuzosin (10 micromol/l). Furthermore, alfuzosin (30 microg/kg, i.v.) partially, but significantly, inhibited cyclophosphamide-induced plasma protein extravasation in the rat urinary bladder. Phenylephrine-induced Ca(2+) mobilization in cultured dorsal root ganglia neurons was exaggerated by pretreating the rats in vivo with cyclophosphamide. Finally, cyclophosphamide increased c-fos expression in the rat lumbar spinal cord. Also these in vitro and in vivo effects were inhibited by pretreatment with alfuzosin.

CONCLUSIONS

Alpha(1)-adrenoceptors are functionally expressed by capsaicin-sensitive, nociceptive, primary sensory neurons of the rat urinary tract, and their activation may contribute to signal irritative and nociceptive responses arising from the urinary tract. It is possible that, at least, part of the beneficial effects of alpha(1)-adrenoceptor antagonists in the amelioration of storage symptoms in the lower urinary tract derives from their inhibitory effect on neurogenic inflammatory responses.

Authors+Show Affiliations

Interdisciplinary Centre of Excellence for the Study of Inflammation, University of Ferrara, Ferrara, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17240043

Citation

Trevisani, Marcello, et al. "The Influence of Alpha1-adrenoreceptors On Neuropeptide Release From Primary Sensory Neurons of the Lower Urinary Tract." European Urology, vol. 52, no. 3, 2007, pp. 901-8.
Trevisani M, Campi B, Gatti R, et al. The influence of alpha1-adrenoreceptors on neuropeptide release from primary sensory neurons of the lower urinary tract. Eur Urol. 2007;52(3):901-8.
Trevisani, M., Campi, B., Gatti, R., André, E., Materazzi, S., Nicoletti, P., Gazzieri, D., & Geppetti, P. (2007). The influence of alpha1-adrenoreceptors on neuropeptide release from primary sensory neurons of the lower urinary tract. European Urology, 52(3), 901-8.
Trevisani M, et al. The Influence of Alpha1-adrenoreceptors On Neuropeptide Release From Primary Sensory Neurons of the Lower Urinary Tract. Eur Urol. 2007;52(3):901-8. PubMed PMID: 17240043.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The influence of alpha1-adrenoreceptors on neuropeptide release from primary sensory neurons of the lower urinary tract. AU - Trevisani,Marcello, AU - Campi,Barbara, AU - Gatti,Raffaele, AU - André,Eunice, AU - Materazzi,Serena, AU - Nicoletti,Paola, AU - Gazzieri,David, AU - Geppetti,Pierangelo, Y1 - 2007/01/12/ PY - 2006/09/21/received PY - 2007/01/04/accepted PY - 2007/1/24/pubmed PY - 2007/12/7/medline PY - 2007/1/24/entrez SP - 901 EP - 8 JF - European urology JO - Eur. Urol. VL - 52 IS - 3 N2 - OBJECTIVES: Adrenergic alpha(1)-receptors agonists and antagonists have been reported to increase and reduce, respectively, neurogenic inflammatory responses mediated by capsaicin-sensitive sensory neurons. However, the precise role and localization of the alpha(1)-adrenoceptors involved in these effects are not known. METHODS: We have studied in the rat whether functional alpha(1)-adrenoreceptors are expressed in primary sensory neurons, and whether they regulate neurogenic inflammation and nociceptive responses in the urinary bladder. RESULTS: The alpha(1)-adrenoreceptor agonist phenylephrine (1 micromol/l) (1) mobilized intracellular Ca(2+) in cultured lumbar and sacral dorsal root ganglia neurons, (2) caused the release of substance P (SP) from terminals of capsaicin-sensitive sensory neurons from the lumbar enlargement of the dorsal spinal cord and urinary bladder, and (3) increased plasma protein extravasation in the urinary bladder. All these effects were abolished by the alpha(1)-adrenoceptor antagonist alfuzosin (10 micromol/l). Furthermore, alfuzosin (30 microg/kg, i.v.) partially, but significantly, inhibited cyclophosphamide-induced plasma protein extravasation in the rat urinary bladder. Phenylephrine-induced Ca(2+) mobilization in cultured dorsal root ganglia neurons was exaggerated by pretreating the rats in vivo with cyclophosphamide. Finally, cyclophosphamide increased c-fos expression in the rat lumbar spinal cord. Also these in vitro and in vivo effects were inhibited by pretreatment with alfuzosin. CONCLUSIONS: Alpha(1)-adrenoceptors are functionally expressed by capsaicin-sensitive, nociceptive, primary sensory neurons of the rat urinary tract, and their activation may contribute to signal irritative and nociceptive responses arising from the urinary tract. It is possible that, at least, part of the beneficial effects of alpha(1)-adrenoceptor antagonists in the amelioration of storage symptoms in the lower urinary tract derives from their inhibitory effect on neurogenic inflammatory responses. SN - 0302-2838 UR - https://www.unboundmedicine.com/medline/citation/17240043/The_influence_of_alpha1_adrenoreceptors_on_neuropeptide_release_from_primary_sensory_neurons_of_the_lower_urinary_tract_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0302-2838(07)00019-X DB - PRIME DP - Unbound Medicine ER -