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SAG protects human neuroblastoma SH-SY5Y cells against 1-methyl-4-phenylpyridinium ion (MPP+)-induced cytotoxicity via the downregulation of ROS generation and JNK signaling.
Neurosci Lett. 2007 Feb 14; 413(2):132-6.NL

Abstract

Sensitive to apoptosis gene (SAG), a novel zinc RING finger protein, exhibits anti-apoptotic and antioxidant activity against a variety of redox reagents. In the present study, we have determined that SAG suppresses 1-methyl-4-phenylpyridinium ion (MPP(+))-induced neurotoxicity via the downregulation of ROS generation and c-Jun N-terminal kinase 1 (JNK1) activity. Both transient and constitutively overexpressed SAG were found to inhibit the MPP(+)-induced neurotoxicity of SH-SY5Y neuroblastoma cells. In the SAG-expressing cells, MPP(+) induced ROS generation was suppressed to a significant degree as compared to the cells treated only with MPP(+). MPP(+)-induced JNK1 activation was also determined to be suppressed markedly by SAG. Furthermore, SAG inhibits MEKK1 dependent c-Jun transcription activity in SH-SY5Y cells. Thus, we concluded that SAG is a cellular protective molecule, which appears to function as an antioxidant, suppressing MPP(+)-induced neurotoxicity.

Authors+Show Affiliations

Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Yongbong-dong, Buk-ku, Gwangju 500-757, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17240529

Citation

Kim, Sun-Yee, et al. "SAG Protects Human Neuroblastoma SH-SY5Y Cells Against 1-methyl-4-phenylpyridinium Ion (MPP+)-induced Cytotoxicity Via the Downregulation of ROS Generation and JNK Signaling." Neuroscience Letters, vol. 413, no. 2, 2007, pp. 132-6.
Kim SY, Kim MY, Mo JS, et al. SAG protects human neuroblastoma SH-SY5Y cells against 1-methyl-4-phenylpyridinium ion (MPP+)-induced cytotoxicity via the downregulation of ROS generation and JNK signaling. Neurosci Lett. 2007;413(2):132-6.
Kim, S. Y., Kim, M. Y., Mo, J. S., Park, J. W., & Park, H. S. (2007). SAG protects human neuroblastoma SH-SY5Y cells against 1-methyl-4-phenylpyridinium ion (MPP+)-induced cytotoxicity via the downregulation of ROS generation and JNK signaling. Neuroscience Letters, 413(2), 132-6.
Kim SY, et al. SAG Protects Human Neuroblastoma SH-SY5Y Cells Against 1-methyl-4-phenylpyridinium Ion (MPP+)-induced Cytotoxicity Via the Downregulation of ROS Generation and JNK Signaling. Neurosci Lett. 2007 Feb 14;413(2):132-6. PubMed PMID: 17240529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SAG protects human neuroblastoma SH-SY5Y cells against 1-methyl-4-phenylpyridinium ion (MPP+)-induced cytotoxicity via the downregulation of ROS generation and JNK signaling. AU - Kim,Sun-Yee, AU - Kim,Mi-Yeon, AU - Mo,Jung-Soon, AU - Park,Jeen-Woo, AU - Park,Hee-Sae, Y1 - 2007/01/08/ PY - 2006/08/07/received PY - 2006/11/07/revised PY - 2006/11/24/accepted PY - 2007/1/24/pubmed PY - 2007/4/12/medline PY - 2007/1/24/entrez SP - 132 EP - 6 JF - Neuroscience letters JO - Neurosci Lett VL - 413 IS - 2 N2 - Sensitive to apoptosis gene (SAG), a novel zinc RING finger protein, exhibits anti-apoptotic and antioxidant activity against a variety of redox reagents. In the present study, we have determined that SAG suppresses 1-methyl-4-phenylpyridinium ion (MPP(+))-induced neurotoxicity via the downregulation of ROS generation and c-Jun N-terminal kinase 1 (JNK1) activity. Both transient and constitutively overexpressed SAG were found to inhibit the MPP(+)-induced neurotoxicity of SH-SY5Y neuroblastoma cells. In the SAG-expressing cells, MPP(+) induced ROS generation was suppressed to a significant degree as compared to the cells treated only with MPP(+). MPP(+)-induced JNK1 activation was also determined to be suppressed markedly by SAG. Furthermore, SAG inhibits MEKK1 dependent c-Jun transcription activity in SH-SY5Y cells. Thus, we concluded that SAG is a cellular protective molecule, which appears to function as an antioxidant, suppressing MPP(+)-induced neurotoxicity. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/17240529/SAG_protects_human_neuroblastoma_SH_SY5Y_cells_against_1_methyl_4_phenylpyridinium_ion__MPP+__induced_cytotoxicity_via_the_downregulation_of_ROS_generation_and_JNK_signaling_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(06)01282-1 DB - PRIME DP - Unbound Medicine ER -