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Discrete roles of a microsomal linoleate desaturase gene in olive identified by spatiotemporal transcriptional analysis.
Tree Physiol. 2007 Apr; 27(4):481-90.TP

Abstract

The relative abundance of alpha-linolenic (alpha-LeA) compared with linoleic acid is associated with the developmental stage and the plant species and is proposed to have important physiological effects on both vegetative and reproductive plant development. The enzymes responsible for catalyzing the conversion of linoleic acid to alpha-LeA, the omega-3 fatty acid desaturases (FADs), are localized in the plastid or the endoplasmic reticulum (ER). Here we present the isolation of an ER-type omega-3 FAD gene (OeFAD3) from olive (Olea europaea L.). Expression patterns of OeFAD3 in different seed tissues and mesocarps during olive fruit development showed that its contribution to olive oil biosynthesis and modification is minimal. Regulation of OeFAD3 differed from that of its plastidial counterpart, being preferentially expressed in proliferating tissues, in concert with the active membrane biogenesis required for cell division. Trienoic acid-deficient Arabidopsis mutants are male sterile, because alpha-LeA-derived jasmonic acid (JA) is required for pollen development. However, the upregulation of OeFAD3 in different pistil tissues, particularly in vascular bundles and ovaries, rather than in anthers, implies a critical role of alpha-LeA in female gametophyte development in olive, corroborating results from JA-defective tomato mutants that are female sterile but not male sterile.

Authors+Show Affiliations

Laboratory of Molecular Biology, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17241990

Citation

Banilas, Georgios, et al. "Discrete Roles of a Microsomal Linoleate Desaturase Gene in Olive Identified By Spatiotemporal Transcriptional Analysis." Tree Physiology, vol. 27, no. 4, 2007, pp. 481-90.
Banilas G, Nikiforiadis A, Makariti I, et al. Discrete roles of a microsomal linoleate desaturase gene in olive identified by spatiotemporal transcriptional analysis. Tree Physiol. 2007;27(4):481-90.
Banilas, G., Nikiforiadis, A., Makariti, I., Moressis, A., & Hatzopoulos, P. (2007). Discrete roles of a microsomal linoleate desaturase gene in olive identified by spatiotemporal transcriptional analysis. Tree Physiology, 27(4), 481-90.
Banilas G, et al. Discrete Roles of a Microsomal Linoleate Desaturase Gene in Olive Identified By Spatiotemporal Transcriptional Analysis. Tree Physiol. 2007;27(4):481-90. PubMed PMID: 17241990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Discrete roles of a microsomal linoleate desaturase gene in olive identified by spatiotemporal transcriptional analysis. AU - Banilas,Georgios, AU - Nikiforiadis,Alkis, AU - Makariti,Ifigenia, AU - Moressis,Anastassios, AU - Hatzopoulos,Polydefkis, PY - 2007/1/24/pubmed PY - 2007/6/1/medline PY - 2007/1/24/entrez SP - 481 EP - 90 JF - Tree physiology JO - Tree Physiol VL - 27 IS - 4 N2 - The relative abundance of alpha-linolenic (alpha-LeA) compared with linoleic acid is associated with the developmental stage and the plant species and is proposed to have important physiological effects on both vegetative and reproductive plant development. The enzymes responsible for catalyzing the conversion of linoleic acid to alpha-LeA, the omega-3 fatty acid desaturases (FADs), are localized in the plastid or the endoplasmic reticulum (ER). Here we present the isolation of an ER-type omega-3 FAD gene (OeFAD3) from olive (Olea europaea L.). Expression patterns of OeFAD3 in different seed tissues and mesocarps during olive fruit development showed that its contribution to olive oil biosynthesis and modification is minimal. Regulation of OeFAD3 differed from that of its plastidial counterpart, being preferentially expressed in proliferating tissues, in concert with the active membrane biogenesis required for cell division. Trienoic acid-deficient Arabidopsis mutants are male sterile, because alpha-LeA-derived jasmonic acid (JA) is required for pollen development. However, the upregulation of OeFAD3 in different pistil tissues, particularly in vascular bundles and ovaries, rather than in anthers, implies a critical role of alpha-LeA in female gametophyte development in olive, corroborating results from JA-defective tomato mutants that are female sterile but not male sterile. SN - 0829-318X UR - https://www.unboundmedicine.com/medline/citation/17241990/Discrete_roles_of_a_microsomal_linoleate_desaturase_gene_in_olive_identified_by_spatiotemporal_transcriptional_analysis_ L2 - https://academic.oup.com/treephys/article-lookup/doi/10.1093/treephys/27.4.481 DB - PRIME DP - Unbound Medicine ER -