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Changes of testicular aromatase expression during fetal development in male pigs (Sus scrofa).
Reproduction. 2007 Jan; 133(1):323-30.R

Abstract

Male pig fetuses secrete considerable amounts of estrogens, but the location of aromatase activity within the fetal testis is not known. The location of aromatase expression was investigated by immunocytochemistry in fetal testes from week 6 (n = 5), weeks 10, 13, and 15 (each: n = 6) of gestation and additionally in neonates (n = 4). Blood was sampled from the umbilical artery of fetuses and jugular vein of neonates. Histological evaluation of testes involved morphological criteria and counting of Leydig cells, Sertoli cells, and gonocytes. Aromatase activity was localized immunocytochemically and quantified by the percentage of positive stained cells within the same cell type. Aromatase expression was further characterized by quantitative RT-PCR. Concentrations of estrogens, testosterone, FSH, and LH were measured in blood plasma. Total estrogens increased from week 10 to a maximum of 31.03 nmol/l in week 15. Increased testosterone concentrations were only measured at week 6 and were paralleled by slightly elevated estrogens. Thereafter, testosterone dropped and was low throughout. The increase of estrogens was not paralleled by a similar increase of FSH and LH but was related to the increase of the total number of Leydig cells. This increase was also found for mRNA expression. Both Leydig cells and gonocytes were identified as contributors to estrogen formation. Gonocytes were the main source of aromatase at week 10, when gene expression by Leydig cells is low due to the preparation of a wave of Leydig cell mitosis.

Authors+Show Affiliations

Institut für Tierhaltung und Tierzüchtung, Universität Hohenheim, Garbenstr 17, 70599 Stuttgart, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17244757

Citation

Haeussler, Susanne, et al. "Changes of Testicular Aromatase Expression During Fetal Development in Male Pigs (Sus Scrofa)." Reproduction (Cambridge, England), vol. 133, no. 1, 2007, pp. 323-30.
Haeussler S, Wagner A, Welter H, et al. Changes of testicular aromatase expression during fetal development in male pigs (Sus scrofa). Reproduction. 2007;133(1):323-30.
Haeussler, S., Wagner, A., Welter, H., & Claus, R. (2007). Changes of testicular aromatase expression during fetal development in male pigs (Sus scrofa). Reproduction (Cambridge, England), 133(1), 323-30.
Haeussler S, et al. Changes of Testicular Aromatase Expression During Fetal Development in Male Pigs (Sus Scrofa). Reproduction. 2007;133(1):323-30. PubMed PMID: 17244757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes of testicular aromatase expression during fetal development in male pigs (Sus scrofa). AU - Haeussler,Susanne, AU - Wagner,Anna, AU - Welter,Harald, AU - Claus,Rolf, PY - 2007/1/25/pubmed PY - 2007/8/19/medline PY - 2007/1/25/entrez SP - 323 EP - 30 JF - Reproduction (Cambridge, England) JO - Reproduction VL - 133 IS - 1 N2 - Male pig fetuses secrete considerable amounts of estrogens, but the location of aromatase activity within the fetal testis is not known. The location of aromatase expression was investigated by immunocytochemistry in fetal testes from week 6 (n = 5), weeks 10, 13, and 15 (each: n = 6) of gestation and additionally in neonates (n = 4). Blood was sampled from the umbilical artery of fetuses and jugular vein of neonates. Histological evaluation of testes involved morphological criteria and counting of Leydig cells, Sertoli cells, and gonocytes. Aromatase activity was localized immunocytochemically and quantified by the percentage of positive stained cells within the same cell type. Aromatase expression was further characterized by quantitative RT-PCR. Concentrations of estrogens, testosterone, FSH, and LH were measured in blood plasma. Total estrogens increased from week 10 to a maximum of 31.03 nmol/l in week 15. Increased testosterone concentrations were only measured at week 6 and were paralleled by slightly elevated estrogens. Thereafter, testosterone dropped and was low throughout. The increase of estrogens was not paralleled by a similar increase of FSH and LH but was related to the increase of the total number of Leydig cells. This increase was also found for mRNA expression. Both Leydig cells and gonocytes were identified as contributors to estrogen formation. Gonocytes were the main source of aromatase at week 10, when gene expression by Leydig cells is low due to the preparation of a wave of Leydig cell mitosis. SN - 1470-1626 UR - https://www.unboundmedicine.com/medline/citation/17244757/Changes_of_testicular_aromatase_expression_during_fetal_development_in_male_pigs__Sus_scrofa__ L2 - https://rep.bioscientifica.com/doi/10.1530/rep.1.01169 DB - PRIME DP - Unbound Medicine ER -