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Studies on the efficacy of mast cell growth factor (c-kit ligand) in vitro as well as in vivo.
Behring Inst Mitt. 1991 DecBI

Abstract

We tested the ability of recombinant human (rhu) mast cell growth factor (MGF), also known as c-kit ligand, to stimulate the colony formation of human bone marrow cells in semisolid medium alone and in combination with rhu erythropoietin (EPO), rhu Interleukin 3 (IL-3), rhu granulocyte colony stimulating factor (G-CSF) and rhu granulocyte-macrophage colony stimulating factor (GM-CSF). The addition of MGF to cultures containing EPO or EPO + IL-3, GM-CSF and G-CSF, resp., resulted in macroscopic erythroid burst-forming units (BFU-E). Multipotential (colony-forming unit granulocyte, erythroid, monocyte, megakaryocyte [CFU-GEMM]) progenitors were stimulated by MGF in the presence of EPO. Colony-forming unit granulocyte-macrophage (CFU-GM) were activated by MGF only in combination with GM-CSF. The combination of MGF with EPO was used for synergism studies in healthy cynomolgus monkeys. In the chosen concentration MGF alone had no effect on white blood cell (WBC) counts and on platelets, but a slight effect on reticulocytes. EPO by itself increased reticulocyte counts with no effects on WBC or platelets. The combination of both factors resulted in a significant increase of reticulocytes. No other effects were seen. These studies demonstrate the potent synergistic interaction of MGF and other hematopoietic growth factors.

Authors+Show Affiliations

Research Laboratories of Behringwerke AG, Marburg, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1724901

Citation

Hintz-Obertreis, P, et al. "Studies On the Efficacy of Mast Cell Growth Factor (c-kit Ligand) in Vitro as Well as in Vivo." Behring Institute Mitteilungen, 1991, pp. 14-22.
Hintz-Obertreis P, Weinmann E, Seiler FR. Studies on the efficacy of mast cell growth factor (c-kit ligand) in vitro as well as in vivo. Behring Inst Mitt. 1991.
Hintz-Obertreis, P., Weinmann, E., & Seiler, F. R. (1991). Studies on the efficacy of mast cell growth factor (c-kit ligand) in vitro as well as in vivo. Behring Institute Mitteilungen, (90), 14-22.
Hintz-Obertreis P, Weinmann E, Seiler FR. Studies On the Efficacy of Mast Cell Growth Factor (c-kit Ligand) in Vitro as Well as in Vivo. Behring Inst Mitt. 1991;(90)14-22. PubMed PMID: 1724901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on the efficacy of mast cell growth factor (c-kit ligand) in vitro as well as in vivo. AU - Hintz-Obertreis,P, AU - Weinmann,E, AU - Seiler,F R, PY - 1991/12/1/pubmed PY - 1991/12/1/medline PY - 1991/12/1/entrez SP - 14 EP - 22 JF - Behring Institute Mitteilungen JO - Behring Inst Mitt IS - 90 N2 - We tested the ability of recombinant human (rhu) mast cell growth factor (MGF), also known as c-kit ligand, to stimulate the colony formation of human bone marrow cells in semisolid medium alone and in combination with rhu erythropoietin (EPO), rhu Interleukin 3 (IL-3), rhu granulocyte colony stimulating factor (G-CSF) and rhu granulocyte-macrophage colony stimulating factor (GM-CSF). The addition of MGF to cultures containing EPO or EPO + IL-3, GM-CSF and G-CSF, resp., resulted in macroscopic erythroid burst-forming units (BFU-E). Multipotential (colony-forming unit granulocyte, erythroid, monocyte, megakaryocyte [CFU-GEMM]) progenitors were stimulated by MGF in the presence of EPO. Colony-forming unit granulocyte-macrophage (CFU-GM) were activated by MGF only in combination with GM-CSF. The combination of MGF with EPO was used for synergism studies in healthy cynomolgus monkeys. In the chosen concentration MGF alone had no effect on white blood cell (WBC) counts and on platelets, but a slight effect on reticulocytes. EPO by itself increased reticulocyte counts with no effects on WBC or platelets. The combination of both factors resulted in a significant increase of reticulocytes. No other effects were seen. These studies demonstrate the potent synergistic interaction of MGF and other hematopoietic growth factors. SN - 0301-0457 UR - https://www.unboundmedicine.com/medline/citation/1724901/Studies_on_the_efficacy_of_mast_cell_growth_factor__c_kit_ligand__in_vitro_as_well_as_in_vivo_ L2 - https://medlineplus.gov/stemcells.html DB - PRIME DP - Unbound Medicine ER -