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Stobadine protects rat kidney against ischaemia/reperfusion injury.
Clin Exp Pharmacol Physiol. 2007 Mar; 34(3):210-6.CE

Abstract

1. Ischaemia-reperfusion (I/R) injury, one of the main causes of acute renal failure, still needs satisfactory treatment for routine clinical application. Stobadine, a novel synthetic pyridoindole anti-oxidant, has the ability to reduce tissue injury induced by mechanisms involving reactive oxygen species during I/R. The aim of the present study was to determine the effects of stobadine on renal I/R injury. 2. Forty male Wistar rats were randomly divided into four groups as follows: sham, I/R, stobadine treated and I/R + stobadine treated. Stobadine (2 mg/kg, i.v.) was given intravenously to two groups of rats. The stobadine-treated group was treated with stobadine following sham operation before the abdominal wall was closed, whereas the I/R + stobadine group received stobadine at the beginning of reperfusion. Renal I/R was achieved by occluding the renal arteries bilaterally for 40 min, followed by 6 h reperfusion. Immediately thereafter, blood was drawn and tissue samples were harvested to assess: (i) serum levels of blood urea nitrogen and creatinine; (ii) serum and/or tissue levels of malondialdehyde (MDA), glutathione (GSH), glucose 6-phosphate dehydrogenase (G-6PD), 6-phosphogluconate dehydrogenase (6-PGD), glutathione reductase (GR) and glutathione peroxidase (GPx); (iii) renal morphology; and (iv) immunohistochemical staining for P-selectin. 3. Stobadine was able to significantly attenuate the renal dysfunction as a result of renal I/R injury. Ischaemia-reperfusion resulted in a significant increase in serum and kidney MDA levels and a decrease in serum and kidney GSH. Stobadine treatment at the beginning of reperfusion attenuated both the increased MDA levels and decreased GSH secondary to I/R injury. In addition, the decreased G-6PD activity observed after I/R was significantly attenuated by stobadine treatment. Stobadine did not alter 6-PGD activity after I/R. Neither GR nor GPx activity was significantly changed in the I/R alone or the I/R + stobadine groups compared with the sham group. In addition, stobadine decreased the morphological deterioration and high P-selectin immunoreactivity secondary to renal I/R injury. 4. A pyridoindole anti-oxidant, stobadine exerts a renal protective effect in renal I/R injury, which is probably due to its radical-scavenging and anti-oxidant activities.

Authors+Show Affiliations

Department of Nephrology, Gazi University Faculty of Medicine, Ankara, Turkey. galip_guz@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17250641

Citation

Guz, Galip, et al. "Stobadine Protects Rat Kidney Against Ischaemia/reperfusion Injury." Clinical and Experimental Pharmacology & Physiology, vol. 34, no. 3, 2007, pp. 210-6.
Guz G, Demirogullari B, Ulusu NN, et al. Stobadine protects rat kidney against ischaemia/reperfusion injury. Clin Exp Pharmacol Physiol. 2007;34(3):210-6.
Guz, G., Demirogullari, B., Ulusu, N. N., Dogu, C., Demirtola, A., Kavutcu, M., Omeroglu, S., Stefek, M., & Karasu, C. (2007). Stobadine protects rat kidney against ischaemia/reperfusion injury. Clinical and Experimental Pharmacology & Physiology, 34(3), 210-6.
Guz G, et al. Stobadine Protects Rat Kidney Against Ischaemia/reperfusion Injury. Clin Exp Pharmacol Physiol. 2007;34(3):210-6. PubMed PMID: 17250641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stobadine protects rat kidney against ischaemia/reperfusion injury. AU - Guz,Galip, AU - Demirogullari,Billur, AU - Ulusu,Nuray N, AU - Dogu,Cihangir, AU - Demirtola,Arzu, AU - Kavutcu,Mustafa, AU - Omeroglu,Suna, AU - Stefek,Milan, AU - Karasu,Cimen, PY - 2007/1/26/pubmed PY - 2007/3/16/medline PY - 2007/1/26/entrez SP - 210 EP - 6 JF - Clinical and experimental pharmacology & physiology JO - Clin Exp Pharmacol Physiol VL - 34 IS - 3 N2 - 1. Ischaemia-reperfusion (I/R) injury, one of the main causes of acute renal failure, still needs satisfactory treatment for routine clinical application. Stobadine, a novel synthetic pyridoindole anti-oxidant, has the ability to reduce tissue injury induced by mechanisms involving reactive oxygen species during I/R. The aim of the present study was to determine the effects of stobadine on renal I/R injury. 2. Forty male Wistar rats were randomly divided into four groups as follows: sham, I/R, stobadine treated and I/R + stobadine treated. Stobadine (2 mg/kg, i.v.) was given intravenously to two groups of rats. The stobadine-treated group was treated with stobadine following sham operation before the abdominal wall was closed, whereas the I/R + stobadine group received stobadine at the beginning of reperfusion. Renal I/R was achieved by occluding the renal arteries bilaterally for 40 min, followed by 6 h reperfusion. Immediately thereafter, blood was drawn and tissue samples were harvested to assess: (i) serum levels of blood urea nitrogen and creatinine; (ii) serum and/or tissue levels of malondialdehyde (MDA), glutathione (GSH), glucose 6-phosphate dehydrogenase (G-6PD), 6-phosphogluconate dehydrogenase (6-PGD), glutathione reductase (GR) and glutathione peroxidase (GPx); (iii) renal morphology; and (iv) immunohistochemical staining for P-selectin. 3. Stobadine was able to significantly attenuate the renal dysfunction as a result of renal I/R injury. Ischaemia-reperfusion resulted in a significant increase in serum and kidney MDA levels and a decrease in serum and kidney GSH. Stobadine treatment at the beginning of reperfusion attenuated both the increased MDA levels and decreased GSH secondary to I/R injury. In addition, the decreased G-6PD activity observed after I/R was significantly attenuated by stobadine treatment. Stobadine did not alter 6-PGD activity after I/R. Neither GR nor GPx activity was significantly changed in the I/R alone or the I/R + stobadine groups compared with the sham group. In addition, stobadine decreased the morphological deterioration and high P-selectin immunoreactivity secondary to renal I/R injury. 4. A pyridoindole anti-oxidant, stobadine exerts a renal protective effect in renal I/R injury, which is probably due to its radical-scavenging and anti-oxidant activities. SN - 0305-1870 UR - https://www.unboundmedicine.com/medline/citation/17250641/Stobadine_protects_rat_kidney_against_ischaemia/reperfusion_injury_ L2 - https://doi.org/10.1111/j.1440-1681.2007.04574.x DB - PRIME DP - Unbound Medicine ER -