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Diagnosis of Parietaria judaica pollen allergy using natural and recombinant Par j 1 and Par j 2 allergens.
Clin Exp Allergy 2007; 37(2):243-50CE

Abstract

BACKGROUND

Parietaria judaica pollen is one of the main causes of allergic diseases in the Mediterranean area and contains two major allergens, called Par j 1 and Par j 2.

OBJECTIVE

To evaluate the diagnostic potential of natural and recombinant forms of Par j 1 and Par j 2 in comparison with standardized P. judaica pollen extract.

METHODS

Thirty patients allergic to P. judaica pollen and 15 control patients were investigated. Skin prick tests and determination of specific IgE levels were performed with commercial P. judaica extract, natural Par j 1 and Par j 2, and recombinant forms of both allergens expressed in P. pastoris.

RESULTS

The whole group of patients with allergy to P. judaica had a positive skin test reaction to purified nPar j 1-Par j 2 and rPar j 2 at 5 microg/mL, and no false-positive reactions were detected. Natural and recombinant Par j 1 and Par j 2 showed no significantly different responses in skin tests compared with P. judaica extract. A high correlation was found between the serum-specific IgE levels to P. judaica extract vs. natural (R=0.996; P<0.001) and recombinant allergens (R=0.887 and 0.982 for rPar j 1 and rPar j 2, respectively; P<0.001). rPar j 2 displayed a 100% sensitivity and specificity among P. judaica-allergic patients.

CONCLUSIONS

In vivo and in vitro diagnosis of P. judaica pollen allergy could be simplified using rPar j 2. This protein showed comparable IgE response and skin prick reactivity with those produced by P. judaica pollen extract.

Authors+Show Affiliations

Research & Development Department, Bial-Arístegui, Bilbao, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17250697

Citation

González-Rioja, R, et al. "Diagnosis of Parietaria Judaica Pollen Allergy Using Natural and Recombinant Par J 1 and Par J 2 Allergens." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 37, no. 2, 2007, pp. 243-50.
González-Rioja R, Ferrer A, Arilla MC, et al. Diagnosis of Parietaria judaica pollen allergy using natural and recombinant Par j 1 and Par j 2 allergens. Clin Exp Allergy. 2007;37(2):243-50.
González-Rioja, R., Ferrer, A., Arilla, M. C., Ibarrola, I., Viguera, A. R., Andreu, C., ... Asturias, J. A. (2007). Diagnosis of Parietaria judaica pollen allergy using natural and recombinant Par j 1 and Par j 2 allergens. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 37(2), pp. 243-50.
González-Rioja R, et al. Diagnosis of Parietaria Judaica Pollen Allergy Using Natural and Recombinant Par J 1 and Par J 2 Allergens. Clin Exp Allergy. 2007;37(2):243-50. PubMed PMID: 17250697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of Parietaria judaica pollen allergy using natural and recombinant Par j 1 and Par j 2 allergens. AU - González-Rioja,R, AU - Ferrer,A, AU - Arilla,M C, AU - Ibarrola,I, AU - Viguera,A R, AU - Andreu,C, AU - Martínez,A, AU - Asturias,J A, PY - 2007/1/26/pubmed PY - 2007/8/19/medline PY - 2007/1/26/entrez SP - 243 EP - 50 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin. Exp. Allergy VL - 37 IS - 2 N2 - BACKGROUND: Parietaria judaica pollen is one of the main causes of allergic diseases in the Mediterranean area and contains two major allergens, called Par j 1 and Par j 2. OBJECTIVE: To evaluate the diagnostic potential of natural and recombinant forms of Par j 1 and Par j 2 in comparison with standardized P. judaica pollen extract. METHODS: Thirty patients allergic to P. judaica pollen and 15 control patients were investigated. Skin prick tests and determination of specific IgE levels were performed with commercial P. judaica extract, natural Par j 1 and Par j 2, and recombinant forms of both allergens expressed in P. pastoris. RESULTS: The whole group of patients with allergy to P. judaica had a positive skin test reaction to purified nPar j 1-Par j 2 and rPar j 2 at 5 microg/mL, and no false-positive reactions were detected. Natural and recombinant Par j 1 and Par j 2 showed no significantly different responses in skin tests compared with P. judaica extract. A high correlation was found between the serum-specific IgE levels to P. judaica extract vs. natural (R=0.996; P<0.001) and recombinant allergens (R=0.887 and 0.982 for rPar j 1 and rPar j 2, respectively; P<0.001). rPar j 2 displayed a 100% sensitivity and specificity among P. judaica-allergic patients. CONCLUSIONS: In vivo and in vitro diagnosis of P. judaica pollen allergy could be simplified using rPar j 2. This protein showed comparable IgE response and skin prick reactivity with those produced by P. judaica pollen extract. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/17250697/Diagnosis_of_Parietaria_judaica_pollen_allergy_using_natural_and_recombinant_Par_j_1_and_Par_j_2_allergens_ L2 - https://doi.org/10.1111/j.1365-2222.2007.02643.x DB - PRIME DP - Unbound Medicine ER -