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Identification of a plane pollen lipid transfer protein (Pla a 3) and its immunological relation to the peach lipid-transfer protein, Pru p 3.
Clin Exp Allergy 2007; 37(2):261-9CE

Abstract

BACKGROUND

An association between plane tree pollen allergy and plant food allergy has been described, but the cross-reacting allergens have not yet been identified. The aim of this study was the identification of homologous non-specific lipid-transfer proteins (nsLTPs) in plane pollen, and to investigate its immunological relationship with the peach LTP, Pru p 3.

METHODS

Three different patient groups were recruited in Spain: 22 plane pollen-allergic patients without food allergy (A), 36 plane pollen-allergic patients with peach allergy (B) and 10 peach-allergic patients without plane pollen allergy (C). Proteins from plane pollen extract were fractionated by ion-exchange and reversed-phase chromatography. Further methods applied were N-terminal amino acid sequence analysis, immunoblotting, enzyme allergosorbent test, CAP and basophil histamine release assays.

RESULTS

A 10 kDa IgE-reactive protein was purified from plane pollen and identified as nsLTP. Pla a 3 was characterized as a minor allergen (27.3%) in plane pollen-allergic patients without food allergy (A) and as a major allergen in plane pollen-allergic patients with peach allergy (B) showing a prevalence of IgE-reactivity of 63.8%. Group B contained patients sensitized to Pru p 3 without IgE-reactivity to plane-LTP (16.6%). By contrast, Pla a 3 IgE-reactive patients without sensitization to Pru p 3 could be found (16.6%). The sera of patients sensitized to both LTPs (50%), Pla a 3 and Pru p 3, showed different biological activity in histamine release assay: depending on individual patient's sera tested, Pla a 3 showed a similar, a stronger or a weaker allergenic potency in comparison with Pru p 3.

CONCLUSIONS

Plane LTP is a major allergen in plane pollen-allergic patients with peach allergy recruited in the Mediterranean area. The results of histamine release tests and different IgE-binding profiles pointed towards the existence of species-specific IgE epitopes. Likewise, no general conclusion on the sensitizer could be made.

Authors+Show Affiliations

Department of Allergology, Paul-Ehrlich-Institut, Langen, Germany. lauir@pei.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17250699

Citation

Lauer, I, et al. "Identification of a Plane Pollen Lipid Transfer Protein (Pla a 3) and Its Immunological Relation to the Peach Lipid-transfer Protein, Pru P 3." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 37, no. 2, 2007, pp. 261-9.
Lauer I, Miguel-Moncin MS, Abel T, et al. Identification of a plane pollen lipid transfer protein (Pla a 3) and its immunological relation to the peach lipid-transfer protein, Pru p 3. Clin Exp Allergy. 2007;37(2):261-9.
Lauer, I., Miguel-Moncin, M. S., Abel, T., Foetisch, K., Hartz, C., Fortunato, D., ... Scheurer, S. (2007). Identification of a plane pollen lipid transfer protein (Pla a 3) and its immunological relation to the peach lipid-transfer protein, Pru p 3. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 37(2), pp. 261-9.
Lauer I, et al. Identification of a Plane Pollen Lipid Transfer Protein (Pla a 3) and Its Immunological Relation to the Peach Lipid-transfer Protein, Pru P 3. Clin Exp Allergy. 2007;37(2):261-9. PubMed PMID: 17250699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of a plane pollen lipid transfer protein (Pla a 3) and its immunological relation to the peach lipid-transfer protein, Pru p 3. AU - Lauer,I, AU - Miguel-Moncin,M S, AU - Abel,T, AU - Foetisch,K, AU - Hartz,C, AU - Fortunato,D, AU - Cistero-Bahima,A, AU - Vieths,S, AU - Scheurer,S, PY - 2007/1/26/pubmed PY - 2007/8/19/medline PY - 2007/1/26/entrez SP - 261 EP - 9 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin. Exp. Allergy VL - 37 IS - 2 N2 - BACKGROUND: An association between plane tree pollen allergy and plant food allergy has been described, but the cross-reacting allergens have not yet been identified. The aim of this study was the identification of homologous non-specific lipid-transfer proteins (nsLTPs) in plane pollen, and to investigate its immunological relationship with the peach LTP, Pru p 3. METHODS: Three different patient groups were recruited in Spain: 22 plane pollen-allergic patients without food allergy (A), 36 plane pollen-allergic patients with peach allergy (B) and 10 peach-allergic patients without plane pollen allergy (C). Proteins from plane pollen extract were fractionated by ion-exchange and reversed-phase chromatography. Further methods applied were N-terminal amino acid sequence analysis, immunoblotting, enzyme allergosorbent test, CAP and basophil histamine release assays. RESULTS: A 10 kDa IgE-reactive protein was purified from plane pollen and identified as nsLTP. Pla a 3 was characterized as a minor allergen (27.3%) in plane pollen-allergic patients without food allergy (A) and as a major allergen in plane pollen-allergic patients with peach allergy (B) showing a prevalence of IgE-reactivity of 63.8%. Group B contained patients sensitized to Pru p 3 without IgE-reactivity to plane-LTP (16.6%). By contrast, Pla a 3 IgE-reactive patients without sensitization to Pru p 3 could be found (16.6%). The sera of patients sensitized to both LTPs (50%), Pla a 3 and Pru p 3, showed different biological activity in histamine release assay: depending on individual patient's sera tested, Pla a 3 showed a similar, a stronger or a weaker allergenic potency in comparison with Pru p 3. CONCLUSIONS: Plane LTP is a major allergen in plane pollen-allergic patients with peach allergy recruited in the Mediterranean area. The results of histamine release tests and different IgE-binding profiles pointed towards the existence of species-specific IgE epitopes. Likewise, no general conclusion on the sensitizer could be made. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/17250699/Identification_of_a_plane_pollen_lipid_transfer_protein__Pla_a_3__and_its_immunological_relation_to_the_peach_lipid_transfer_protein_Pru_p_3_ L2 - https://doi.org/10.1111/j.1365-2222.2007.02653.x DB - PRIME DP - Unbound Medicine ER -