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The pattern electroretinogram as a tool to monitor progressive retinal ganglion cell dysfunction in the DBA/2J mouse model of glaucoma.
Invest Ophthalmol Vis Sci. 2007 Feb; 48(2):745-51.IO

Abstract

PURPOSE

To determine the baseline characteristics, reliability, and dynamic range of the pattern electroretinogram (PERG) as a tool to monitor progressive RGC dysfunction in the DBA/2J mouse model of glaucoma with spontaneously elevated intraocular pressure (IOP).

METHODS

PERGs were recorded from 56 undilated eyes of 28 anesthetized (ketamine-xylazine-acepromazine) DBA/2J mice of different ages (2-4 months, n = 44 eyes; 12-14 months, n = 12 eyes) in response to contrast reversal of gratings that maximize PERG amplitude (95% contrast, 1-Hz reversal, 0.05 cyc/deg spatial frequency, 50 degrees x 56 degrees field size). Robust averaging (1800 sweeps) was used to isolate PERG from background noise. Cone-driven ERGs in response to diffuse light flashes superimposed on a rod-adapting background (FERG) were also recorded.

RESULTS

PERGs had consistent waveforms and were reproducible across batches of mice and operators. In 2- to 4-month-old mice (prehypertensive stage), the PERG amplitude (mean, 8.15 +/- 0.4 microV [SEM]) was considerably larger than the noise (mean 1.18 +/- 0.1 microV). The test-retest variability (two different sessions 1 week apart) and interocular asymmetry of PERG amplitude was approximately 30%, and that of PERG latency was approximately 17%. In 12- to 14-month-old mice (advanced hypertensive stage) the PERG amplitude (mean, 1.29 +/- 0.12 microV) was close to that of noise. In 12- to 14-month-old mice the FERG was reduced to a lesser extent compared with the PERG.

CONCLUSIONS

The PERG has an adequate signal-to-noise ratio, reproducibility, and dynamic range to monitor the progression of functional changes in the inner retina in DBA/2J mice.

Authors+Show Affiliations

Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida 33136, USA. vporciatti@med.miami.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17251473

Citation

Porciatti, Vittorio, et al. "The Pattern Electroretinogram as a Tool to Monitor Progressive Retinal Ganglion Cell Dysfunction in the DBA/2J Mouse Model of Glaucoma." Investigative Ophthalmology & Visual Science, vol. 48, no. 2, 2007, pp. 745-51.
Porciatti V, Saleh M, Nagaraju M. The pattern electroretinogram as a tool to monitor progressive retinal ganglion cell dysfunction in the DBA/2J mouse model of glaucoma. Invest Ophthalmol Vis Sci. 2007;48(2):745-51.
Porciatti, V., Saleh, M., & Nagaraju, M. (2007). The pattern electroretinogram as a tool to monitor progressive retinal ganglion cell dysfunction in the DBA/2J mouse model of glaucoma. Investigative Ophthalmology & Visual Science, 48(2), 745-51.
Porciatti V, Saleh M, Nagaraju M. The Pattern Electroretinogram as a Tool to Monitor Progressive Retinal Ganglion Cell Dysfunction in the DBA/2J Mouse Model of Glaucoma. Invest Ophthalmol Vis Sci. 2007;48(2):745-51. PubMed PMID: 17251473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The pattern electroretinogram as a tool to monitor progressive retinal ganglion cell dysfunction in the DBA/2J mouse model of glaucoma. AU - Porciatti,Vittorio, AU - Saleh,Maher, AU - Nagaraju,Mahesh, PY - 2007/1/26/pubmed PY - 2007/3/3/medline PY - 2007/1/26/entrez SP - 745 EP - 51 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 48 IS - 2 N2 - PURPOSE: To determine the baseline characteristics, reliability, and dynamic range of the pattern electroretinogram (PERG) as a tool to monitor progressive RGC dysfunction in the DBA/2J mouse model of glaucoma with spontaneously elevated intraocular pressure (IOP). METHODS: PERGs were recorded from 56 undilated eyes of 28 anesthetized (ketamine-xylazine-acepromazine) DBA/2J mice of different ages (2-4 months, n = 44 eyes; 12-14 months, n = 12 eyes) in response to contrast reversal of gratings that maximize PERG amplitude (95% contrast, 1-Hz reversal, 0.05 cyc/deg spatial frequency, 50 degrees x 56 degrees field size). Robust averaging (1800 sweeps) was used to isolate PERG from background noise. Cone-driven ERGs in response to diffuse light flashes superimposed on a rod-adapting background (FERG) were also recorded. RESULTS: PERGs had consistent waveforms and were reproducible across batches of mice and operators. In 2- to 4-month-old mice (prehypertensive stage), the PERG amplitude (mean, 8.15 +/- 0.4 microV [SEM]) was considerably larger than the noise (mean 1.18 +/- 0.1 microV). The test-retest variability (two different sessions 1 week apart) and interocular asymmetry of PERG amplitude was approximately 30%, and that of PERG latency was approximately 17%. In 12- to 14-month-old mice (advanced hypertensive stage) the PERG amplitude (mean, 1.29 +/- 0.12 microV) was close to that of noise. In 12- to 14-month-old mice the FERG was reduced to a lesser extent compared with the PERG. CONCLUSIONS: The PERG has an adequate signal-to-noise ratio, reproducibility, and dynamic range to monitor the progression of functional changes in the inner retina in DBA/2J mice. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/17251473/The_pattern_electroretinogram_as_a_tool_to_monitor_progressive_retinal_ganglion_cell_dysfunction_in_the_DBA/2J_mouse_model_of_glaucoma_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.06-0733 DB - PRIME DP - Unbound Medicine ER -