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Signal enhancement of surface plasmon resonance based on gold nanoparticle-antibody complex for immunoassay.
J Nanosci Nanotechnol. 2006 Nov; 6(11):3521-5.JN

Abstract

In the immunoassay based on surface plasmon resonance (SPR) system, the signal enhancement was done by means of the conjugate of gold (Au) nanoparticle-antibody fragment. Antibody fragment was prepared for the improved immobilization based on Au-thiol interaction. Through the ellipsometric analysis on surface, the conjugation between Au and antibody fragment was performed in the oriented manner. The optimal fabrication conditions such as concentration and incubation time were determined for the constant size of the fabricated nanoparticle-antibody conjugate. Through the plot of SPR angle difference versus antigen concentration, the linear correlation was achieved, of which the detection limit was 100 fg/ml.

Authors+Show Affiliations

Interdisciplinary Program of Integrated Biotechnology, Sogang University, 1 Shinsoo-Dong, Mapo-Gu, Seoul 121-742, Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17252803

Citation

Lee, Woochang, et al. "Signal Enhancement of Surface Plasmon Resonance Based On Gold Nanoparticle-antibody Complex for Immunoassay." Journal of Nanoscience and Nanotechnology, vol. 6, no. 11, 2006, pp. 3521-5.
Lee W, Oh BK, Kim YW, et al. Signal enhancement of surface plasmon resonance based on gold nanoparticle-antibody complex for immunoassay. J Nanosci Nanotechnol. 2006;6(11):3521-5.
Lee, W., Oh, B. K., Kim, Y. W., & Choi, J. W. (2006). Signal enhancement of surface plasmon resonance based on gold nanoparticle-antibody complex for immunoassay. Journal of Nanoscience and Nanotechnology, 6(11), 3521-5.
Lee W, et al. Signal Enhancement of Surface Plasmon Resonance Based On Gold Nanoparticle-antibody Complex for Immunoassay. J Nanosci Nanotechnol. 2006;6(11):3521-5. PubMed PMID: 17252803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Signal enhancement of surface plasmon resonance based on gold nanoparticle-antibody complex for immunoassay. AU - Lee,Woochang, AU - Oh,Byung-Keun, AU - Kim,Yong-Wan, AU - Choi,Jeong-Woo, PY - 2007/1/27/pubmed PY - 2007/3/30/medline PY - 2007/1/27/entrez SP - 3521 EP - 5 JF - Journal of nanoscience and nanotechnology JO - J Nanosci Nanotechnol VL - 6 IS - 11 N2 - In the immunoassay based on surface plasmon resonance (SPR) system, the signal enhancement was done by means of the conjugate of gold (Au) nanoparticle-antibody fragment. Antibody fragment was prepared for the improved immobilization based on Au-thiol interaction. Through the ellipsometric analysis on surface, the conjugation between Au and antibody fragment was performed in the oriented manner. The optimal fabrication conditions such as concentration and incubation time were determined for the constant size of the fabricated nanoparticle-antibody conjugate. Through the plot of SPR angle difference versus antigen concentration, the linear correlation was achieved, of which the detection limit was 100 fg/ml. SN - 1533-4880 UR - https://www.unboundmedicine.com/medline/citation/17252803/Signal_enhancement_of_surface_plasmon_resonance_based_on_gold_nanoparticle_antibody_complex_for_immunoassay_ L2 - https://antibodies.cancer.gov/detail/CPTC-CD44-1 DB - PRIME DP - Unbound Medicine ER -