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Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study.
Arthritis Res Ther. 2007; 9(1):R6.AR

Abstract

Innervation of the joint with thinly myelinated and unmyelinated sensory nerve fibres is crucial for the occurrence of joint pain. During inflammation in the joint, sensory fibres show changes in the expression of receptors that are important for the activation and sensitization of the neurones and the generation of joint pain. We recently reported that both neurokinin 1 receptors and bradykinin 2 receptors are upregulated in dorsal root ganglion (DRG) neurones (the cell bodies of sensory fibres) in the course of acute and chronic antigen-induced arthritis in the rat. In this study, we begin to address mechanisms of the interaction between fibroblast-like synovial (FLS) cells and sensory neurones by establishing a co-culture system of FLS cells and DRG neurones. The proportion of DRG neurones expressing neurokinin 1 receptor-like immunoreactivity was not altered in the co-culture with FLS cells from normal joints but was significantly upregulated using FLS cells from knee joints of rats with antigen-induced arthritis. The proportion of DRG neurones expressing bradykinin 2 receptors was slightly upregulated in the presence of FLS cells from normal joints but upregulation was more pronounced in DRG neurones co-cultured with FLS cells from acutely inflamed joints. In addition, the expression of the transient receptor potential V1 (TRPV1) receptor, which is involved in inflammation-evoked thermal hyperalgesia, was mainly upregulated by co-culturing DRG neurones with FLS cells from chronically inflamed joints. Upregulation of neurokinin 1 receptors but not of bradykinin 2 and TRPV1 receptors was also observed when only the supernatant of FLS cells from acutely inflamed joint was added to DRG neurones. Addition of indomethacin to co-cultures inhibited the effect of FLS cells from acutely inflamed joints on neurokinin 1 receptor expression, suggesting an important role for prostaglandins. Collectively, these data show that FLS cells are able to induce an upregulation of pain-related receptors in sensory neurones and, thus, they could contribute to the generation of joint pain. Importantly, the influence of FLS cells on DRG neurones is dependent on their state of activity, and soluble factors as well as direct cellular contacts are crucial for their interaction with neurones.

Authors+Show Affiliations

Institute of Physiology, University of Jena, Teichgraben 8, D-07740 Jena, Germany. Gisela.SegondvonBanchet@mti.uni-jena.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17254343

Citation

von Banchet, Gisela Segond, et al. "Fibroblast-like Synovial Cells From Normal and Inflamed Knee Joints Differently Affect the Expression of Pain-related Receptors in Sensory Neurones: a Co-culture Study." Arthritis Research & Therapy, vol. 9, no. 1, 2007, pp. R6.
von Banchet GS, Richter J, Hückel M, et al. Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study. Arthritis Res Ther. 2007;9(1):R6.
von Banchet, G. S., Richter, J., Hückel, M., Rose, C., Bräuer, R., & Schaible, H. G. (2007). Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study. Arthritis Research & Therapy, 9(1), R6.
von Banchet GS, et al. Fibroblast-like Synovial Cells From Normal and Inflamed Knee Joints Differently Affect the Expression of Pain-related Receptors in Sensory Neurones: a Co-culture Study. Arthritis Res Ther. 2007;9(1):R6. PubMed PMID: 17254343.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study. AU - von Banchet,Gisela Segond, AU - Richter,Jonny, AU - Hückel,Marion, AU - Rose,Christina, AU - Bräuer,Rolf, AU - Schaible,Hans-Georg, PY - 2006/08/31/received PY - 2006/12/20/revised PY - 2007/01/25/accepted PY - 2007/1/27/pubmed PY - 2007/5/1/medline PY - 2007/1/27/entrez SP - R6 EP - R6 JF - Arthritis research & therapy JO - Arthritis Res. Ther. VL - 9 IS - 1 N2 - Innervation of the joint with thinly myelinated and unmyelinated sensory nerve fibres is crucial for the occurrence of joint pain. During inflammation in the joint, sensory fibres show changes in the expression of receptors that are important for the activation and sensitization of the neurones and the generation of joint pain. We recently reported that both neurokinin 1 receptors and bradykinin 2 receptors are upregulated in dorsal root ganglion (DRG) neurones (the cell bodies of sensory fibres) in the course of acute and chronic antigen-induced arthritis in the rat. In this study, we begin to address mechanisms of the interaction between fibroblast-like synovial (FLS) cells and sensory neurones by establishing a co-culture system of FLS cells and DRG neurones. The proportion of DRG neurones expressing neurokinin 1 receptor-like immunoreactivity was not altered in the co-culture with FLS cells from normal joints but was significantly upregulated using FLS cells from knee joints of rats with antigen-induced arthritis. The proportion of DRG neurones expressing bradykinin 2 receptors was slightly upregulated in the presence of FLS cells from normal joints but upregulation was more pronounced in DRG neurones co-cultured with FLS cells from acutely inflamed joints. In addition, the expression of the transient receptor potential V1 (TRPV1) receptor, which is involved in inflammation-evoked thermal hyperalgesia, was mainly upregulated by co-culturing DRG neurones with FLS cells from chronically inflamed joints. Upregulation of neurokinin 1 receptors but not of bradykinin 2 and TRPV1 receptors was also observed when only the supernatant of FLS cells from acutely inflamed joint was added to DRG neurones. Addition of indomethacin to co-cultures inhibited the effect of FLS cells from acutely inflamed joints on neurokinin 1 receptor expression, suggesting an important role for prostaglandins. Collectively, these data show that FLS cells are able to induce an upregulation of pain-related receptors in sensory neurones and, thus, they could contribute to the generation of joint pain. Importantly, the influence of FLS cells on DRG neurones is dependent on their state of activity, and soluble factors as well as direct cellular contacts are crucial for their interaction with neurones. SN - 1478-6362 UR - https://www.unboundmedicine.com/medline/citation/17254343/Fibroblast_like_synovial_cells_from_normal_and_inflamed_knee_joints_differently_affect_the_expression_of_pain_related_receptors_in_sensory_neurones:_a_co_culture_study_ L2 - https://arthritis-research.biomedcentral.com/articles/10.1186/ar2112 DB - PRIME DP - Unbound Medicine ER -