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Molecular pathology in the lungs of severe acute respiratory syndrome patients.
Am J Pathol. 2007 Feb; 170(2):538-45.AJ

Abstract

Severe acute respiratory syndrome (SARS) is a novel infectious disease with disastrous clinical consequences, in which the lungs are the major target organs. Previous studies have described the general pathology in the lungs of SARS patients and have identified some of the cell types infected by SARS coronavirus (SARS-CoV). However, at the time of this writing, there were no comprehensive reports of the cellular distribution of the virus in lung tissue. In this study, we have performed double labeling combining in situ hybridization with immunohistochemistry and alternating each of these techniques separately in consecutive sections to evaluate the viral distribution on various cell types in the lungs of seven patients affected with SARS. We found that SARS-CoV was present in bronchial epithelium, type I and II pneumocytes, T lymphocytes, and macrophages/monocytes. For pneumocytes, T lymphocytes, and macrophages, the infection rates were calculated. In addition, our present study is the first to demonstrate infection of endothelial cells and fibroblasts in SARS.

Authors+Show Affiliations

Department of Pathology, Dean, School of Basic Medical Sciences, Peking University Health Science Center, 38 Xueyuan Rd., Beijing 100083, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17255322

Citation

Ye, Juxiang, et al. "Molecular Pathology in the Lungs of Severe Acute Respiratory Syndrome Patients." The American Journal of Pathology, vol. 170, no. 2, 2007, pp. 538-45.
Ye J, Zhang B, Xu J, et al. Molecular pathology in the lungs of severe acute respiratory syndrome patients. Am J Pathol. 2007;170(2):538-45.
Ye, J., Zhang, B., Xu, J., Chang, Q., McNutt, M. A., Korteweg, C., Gong, E., & Gu, J. (2007). Molecular pathology in the lungs of severe acute respiratory syndrome patients. The American Journal of Pathology, 170(2), 538-45.
Ye J, et al. Molecular Pathology in the Lungs of Severe Acute Respiratory Syndrome Patients. Am J Pathol. 2007;170(2):538-45. PubMed PMID: 17255322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular pathology in the lungs of severe acute respiratory syndrome patients. AU - Ye,Juxiang, AU - Zhang,Bo, AU - Xu,Jian, AU - Chang,Qing, AU - McNutt,Michael A, AU - Korteweg,Christine, AU - Gong,Encong, AU - Gu,Jiang, PY - 2007/1/27/pubmed PY - 2007/3/22/medline PY - 2007/1/27/entrez SP - 538 EP - 45 JF - The American journal of pathology JO - Am J Pathol VL - 170 IS - 2 N2 - Severe acute respiratory syndrome (SARS) is a novel infectious disease with disastrous clinical consequences, in which the lungs are the major target organs. Previous studies have described the general pathology in the lungs of SARS patients and have identified some of the cell types infected by SARS coronavirus (SARS-CoV). However, at the time of this writing, there were no comprehensive reports of the cellular distribution of the virus in lung tissue. In this study, we have performed double labeling combining in situ hybridization with immunohistochemistry and alternating each of these techniques separately in consecutive sections to evaluate the viral distribution on various cell types in the lungs of seven patients affected with SARS. We found that SARS-CoV was present in bronchial epithelium, type I and II pneumocytes, T lymphocytes, and macrophages/monocytes. For pneumocytes, T lymphocytes, and macrophages, the infection rates were calculated. In addition, our present study is the first to demonstrate infection of endothelial cells and fibroblasts in SARS. SN - 0002-9440 UR - https://www.unboundmedicine.com/medline/citation/17255322/Molecular_pathology_in_the_lungs_of_severe_acute_respiratory_syndrome_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9440(10)60877-2 DB - PRIME DP - Unbound Medicine ER -