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Voltammetric quantification of adenine and guanine at C60 modified glassy carbon electrodes.
J Nanosci Nanotechnol. 2006 Dec; 6(12):3699-704.JN

Abstract

Voltammetric determination of adenine and guanine has been carried out at C60 modified glassy carbon electrode at physiological pH. Well-defined oxidation peaks were observed with Ep at approximately 990 mV and 692 mV for adenine and guanine respectively. Good colinearity was obtained in the concentration range 0.5 microM to 100.0 microM for adenine and guanine with sensitivity of approximately 0.06 microA microM(-1) and approximately 0.03 microA /microM(-1), respectively. Simultaneous voltammetric determination of adenine and guanine has been described. Recovery studies for adenine and guanine in biological samples were also carried out. Interfering effect of some common metabolites including ascorbic acid has been evaluated. Analytical application of the developed protocol for determination of (G +C)/(A + T) ratio in DNA sample is also described.

Authors+Show Affiliations

Goyal RN
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Singh SP
No affiliation info available

MeSH

AdenineBiosensing TechniquesCrystallizationDNAElectrochemistryFullerenesGuanineMacromolecular SubstancesMaterials TestingMicroelectrodesMolecular ConformationNanostructuresNanotechnologyParticle SizeSurface Properties

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17256318

Citation

Goyal, Rajendra N., and Sudhanshu P. Singh. "Voltammetric Quantification of Adenine and Guanine at C60 Modified Glassy Carbon Electrodes." Journal of Nanoscience and Nanotechnology, vol. 6, no. 12, 2006, pp. 3699-704.
Goyal RN, Singh SP. Voltammetric quantification of adenine and guanine at C60 modified glassy carbon electrodes. J Nanosci Nanotechnol. 2006;6(12):3699-704.
Goyal, R. N., & Singh, S. P. (2006). Voltammetric quantification of adenine and guanine at C60 modified glassy carbon electrodes. Journal of Nanoscience and Nanotechnology, 6(12), 3699-704.
Goyal RN, Singh SP. Voltammetric Quantification of Adenine and Guanine at C60 Modified Glassy Carbon Electrodes. J Nanosci Nanotechnol. 2006;6(12):3699-704. PubMed PMID: 17256318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Voltammetric quantification of adenine and guanine at C60 modified glassy carbon electrodes. AU - Goyal,Rajendra N, AU - Singh,Sudhanshu P, PY - 2007/1/30/pubmed PY - 2007/2/23/medline PY - 2007/1/30/entrez SP - 3699 EP - 704 JF - Journal of nanoscience and nanotechnology JO - J Nanosci Nanotechnol VL - 6 IS - 12 N2 - Voltammetric determination of adenine and guanine has been carried out at C60 modified glassy carbon electrode at physiological pH. Well-defined oxidation peaks were observed with Ep at approximately 990 mV and 692 mV for adenine and guanine respectively. Good colinearity was obtained in the concentration range 0.5 microM to 100.0 microM for adenine and guanine with sensitivity of approximately 0.06 microA microM(-1) and approximately 0.03 microA /microM(-1), respectively. Simultaneous voltammetric determination of adenine and guanine has been described. Recovery studies for adenine and guanine in biological samples were also carried out. Interfering effect of some common metabolites including ascorbic acid has been evaluated. Analytical application of the developed protocol for determination of (G +C)/(A + T) ratio in DNA sample is also described. SN - 1533-4880 UR - https://www.unboundmedicine.com/medline/citation/17256318/Voltammetric_quantification_of_adenine_and_guanine_at_C60_modified_glassy_carbon_electrodes_ DB - PRIME DP - Unbound Medicine ER -