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Induction of CD16+ CD56bright NK cells with antitumour cytotoxicity not only from CD16- CD56bright NK Cells but also from CD16- CD56dim NK cells.
Scand J Immunol. 2007 Feb; 65(2):126-38.SJ

Abstract

The aim of this study was to examine the effect of cytokines on different subsets of NK cells, while especially focusing on CD16(-) CD56(dim) cells and CD16(-) CD56(bright) cells. When human peripheral blood mononuclear cells (PBMC) were cultured with a combination of IL-2, IL-12 and IL-15 for several days, a minor population of CD56(bright) NK cells expanded up to 15%, and also showed potent cytotoxicities against various cancer cells. Sorting experiments revealed that unconventional CD16(-) CD56(+) NK cells (CD16(-) CD56(dim) NK cells and CD16(-) CD56(bright) NK cells, both of which are less than 1% in PBMC) much more vigorously proliferated after cytokine stimulation, whereas predominant CD16(+) CD56(dim) NK cells proliferated poorly. In addition, many of the resting CD16(-) CD56(bright) NK cells developed into CD16(+) CD56(bright) NK cells, and CD16(-) CD56(dim) NK cells developed into CD16(-) CD56(bright) NK cells and also further into CD16(+) CD56(bright) NK cells by the cytokines. CSFE label experiments further substantiated the proliferation capacity of each subset and the developmental process of CD16(+) CD56(bright) NK cells. Both CD16(-) CD56(dim) NK cells and CD16(-) CD56(bright) NK cells produced large amounts of IFN-gamma and Fas-ligands. The CD16(+) CD56(bright) NK cells showed strong cytotoxicities against not only MHC class I (-) but also MHC class I (+) tumours regardless of their expression of CD94/NKG2A presumably because they expressed NKG2D as well as natural cytotoxicity receptors. The proliferation of CD16(+) CD56(bright) NK cells was also induced when PBMC were stimulated with penicillin-treated Streptococcus pyogenes, thus suggesting their role in tumour immunity and bacterial infections.

Authors+Show Affiliations

Department of Urology, National Defense Medical College, Tokorozawa, Saitama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17257217

Citation

Takahashi, E, et al. "Induction of CD16+ CD56bright NK Cells With Antitumour Cytotoxicity Not Only From CD16- CD56bright NK Cells but Also From CD16- CD56dim NK Cells." Scandinavian Journal of Immunology, vol. 65, no. 2, 2007, pp. 126-38.
Takahashi E, Kuranaga N, Satoh K, et al. Induction of CD16+ CD56bright NK cells with antitumour cytotoxicity not only from CD16- CD56bright NK Cells but also from CD16- CD56dim NK cells. Scand J Immunol. 2007;65(2):126-38.
Takahashi, E., Kuranaga, N., Satoh, K., Habu, Y., Shinomiya, N., Asano, T., Seki, S., & Hayakawa, M. (2007). Induction of CD16+ CD56bright NK cells with antitumour cytotoxicity not only from CD16- CD56bright NK Cells but also from CD16- CD56dim NK cells. Scandinavian Journal of Immunology, 65(2), 126-38.
Takahashi E, et al. Induction of CD16+ CD56bright NK Cells With Antitumour Cytotoxicity Not Only From CD16- CD56bright NK Cells but Also From CD16- CD56dim NK Cells. Scand J Immunol. 2007;65(2):126-38. PubMed PMID: 17257217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of CD16+ CD56bright NK cells with antitumour cytotoxicity not only from CD16- CD56bright NK Cells but also from CD16- CD56dim NK cells. AU - Takahashi,E, AU - Kuranaga,N, AU - Satoh,K, AU - Habu,Y, AU - Shinomiya,N, AU - Asano,T, AU - Seki,S, AU - Hayakawa,M, PY - 2007/1/30/pubmed PY - 2007/3/27/medline PY - 2007/1/30/entrez SP - 126 EP - 38 JF - Scandinavian journal of immunology JO - Scand. J. Immunol. VL - 65 IS - 2 N2 - The aim of this study was to examine the effect of cytokines on different subsets of NK cells, while especially focusing on CD16(-) CD56(dim) cells and CD16(-) CD56(bright) cells. When human peripheral blood mononuclear cells (PBMC) were cultured with a combination of IL-2, IL-12 and IL-15 for several days, a minor population of CD56(bright) NK cells expanded up to 15%, and also showed potent cytotoxicities against various cancer cells. Sorting experiments revealed that unconventional CD16(-) CD56(+) NK cells (CD16(-) CD56(dim) NK cells and CD16(-) CD56(bright) NK cells, both of which are less than 1% in PBMC) much more vigorously proliferated after cytokine stimulation, whereas predominant CD16(+) CD56(dim) NK cells proliferated poorly. In addition, many of the resting CD16(-) CD56(bright) NK cells developed into CD16(+) CD56(bright) NK cells, and CD16(-) CD56(dim) NK cells developed into CD16(-) CD56(bright) NK cells and also further into CD16(+) CD56(bright) NK cells by the cytokines. CSFE label experiments further substantiated the proliferation capacity of each subset and the developmental process of CD16(+) CD56(bright) NK cells. Both CD16(-) CD56(dim) NK cells and CD16(-) CD56(bright) NK cells produced large amounts of IFN-gamma and Fas-ligands. The CD16(+) CD56(bright) NK cells showed strong cytotoxicities against not only MHC class I (-) but also MHC class I (+) tumours regardless of their expression of CD94/NKG2A presumably because they expressed NKG2D as well as natural cytotoxicity receptors. The proliferation of CD16(+) CD56(bright) NK cells was also induced when PBMC were stimulated with penicillin-treated Streptococcus pyogenes, thus suggesting their role in tumour immunity and bacterial infections. SN - 0300-9475 UR - https://www.unboundmedicine.com/medline/citation/17257217/Induction_of_CD16+_CD56bright_NK_cells_with_antitumour_cytotoxicity_not_only_from_CD16__CD56bright_NK_Cells_but_also_from_CD16__CD56dim_NK_cells_ L2 - https://doi.org/10.1111/j.1365-3083.2006.01883.x DB - PRIME DP - Unbound Medicine ER -