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Tandem time-of-flight (TOF/TOF) mass spectrometry and the curved-field reflectron.

Abstract

The curved-field reflectron (CFR), when used as the second mass analyzer in a tandem time-of-flight mass spectrometer, provides a design that enables the use of very high energy collision-induced dissociation (CID). Specifically, this is because the wide energy bandwidth of the CFR obviates the need for floating the collision region to decelerate the precursor ions and subsequently reaccelerating product ions to enable reflectron focusing. Here we describe the evolution of tandem instruments based on the CFR, from its introduction in 1993 to the current commercial TOF(2) mass spectrometer from Shimadzu Corporation, and briefly review the history of TOF/TOF instruments. A number of applications are also described. One is the characterization of a C-terminal cleavage of cystatin C that appears to be associated with patients with remitting relapse multiple sclerosis (RRMS). Both surface-enhanced laser desorption/ionization (SELDI) and MALDI were used on a high performance TOF instrument operating in the MS and MS/MS modes. Tandem TOF mass spectrometry has also been used to determine the acetylation sites on histones and on the enzyme, histone acetyl transferase (HAT), responsible for the modification. Acetylation has been determined quantitatively for multiple sites on histone H3 and H4 using a deuteroacetylation method. For a number of closely spaced sites on the histone tail regions, MS/MS enables us to then determine both the order and distribution of acetylation.

Authors+Show Affiliations

Middle Atlantic Mass Spectrometry Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States. rcotter@jhmi.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Review

Language

eng

PubMed ID

17258517

Citation

Cotter, Robert J., et al. "Tandem Time-of-flight (TOF/TOF) Mass Spectrometry and the Curved-field Reflectron." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 855, no. 1, 2007, pp. 2-13.
Cotter RJ, Griffith W, Jelinek C. Tandem time-of-flight (TOF/TOF) mass spectrometry and the curved-field reflectron. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;855(1):2-13.
Cotter, R. J., Griffith, W., & Jelinek, C. (2007). Tandem time-of-flight (TOF/TOF) mass spectrometry and the curved-field reflectron. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 855(1), 2-13.
Cotter RJ, Griffith W, Jelinek C. Tandem Time-of-flight (TOF/TOF) Mass Spectrometry and the Curved-field Reflectron. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;855(1):2-13. PubMed PMID: 17258517.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tandem time-of-flight (TOF/TOF) mass spectrometry and the curved-field reflectron. AU - Cotter,Robert J, AU - Griffith,Wendell, AU - Jelinek,Christine, Y1 - 2007/01/13/ PY - 2006/09/22/received PY - 2007/01/03/revised PY - 2007/01/04/accepted PY - 2007/1/30/pubmed PY - 2007/11/8/medline PY - 2007/1/30/entrez SP - 2 EP - 13 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 855 IS - 1 N2 - The curved-field reflectron (CFR), when used as the second mass analyzer in a tandem time-of-flight mass spectrometer, provides a design that enables the use of very high energy collision-induced dissociation (CID). Specifically, this is because the wide energy bandwidth of the CFR obviates the need for floating the collision region to decelerate the precursor ions and subsequently reaccelerating product ions to enable reflectron focusing. Here we describe the evolution of tandem instruments based on the CFR, from its introduction in 1993 to the current commercial TOF(2) mass spectrometer from Shimadzu Corporation, and briefly review the history of TOF/TOF instruments. A number of applications are also described. One is the characterization of a C-terminal cleavage of cystatin C that appears to be associated with patients with remitting relapse multiple sclerosis (RRMS). Both surface-enhanced laser desorption/ionization (SELDI) and MALDI were used on a high performance TOF instrument operating in the MS and MS/MS modes. Tandem TOF mass spectrometry has also been used to determine the acetylation sites on histones and on the enzyme, histone acetyl transferase (HAT), responsible for the modification. Acetylation has been determined quantitatively for multiple sites on histone H3 and H4 using a deuteroacetylation method. For a number of closely spaced sites on the histone tail regions, MS/MS enables us to then determine both the order and distribution of acetylation. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17258517/Tandem_time_of_flight__TOF/TOF__mass_spectrometry_and_the_curved_field_reflectron_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(07)00013-X DB - PRIME DP - Unbound Medicine ER -