Tags

Type your tag names separated by a space and hit enter

Humoral and cellular immune responses in children given annual immunization with trivalent inactivated influenza vaccine.
Pediatr Infect Dis J 2007; 26(2):107-15PI

Abstract

BACKGROUND

There have been no prior reports of the frequency of circulating influenza-specific, interferon gamma-producing memory CD4+ and CD8+ T-cells in healthy children who have received multiple influenza immunizations.

METHODS

We evaluated 21 previously immunized children, ages 3 to 9 years, before and 1 month after administration of trivalent inactivated influenza vaccine. Frequencies of influenza-specific CD4+ and CD8+ T-cells stimulated with trivalent inactivated influenza vaccine or A/Panama (H3N2) virus were determined by flow cytometry, and antibody responses to vaccine strains and a drifted H3N2 strain were measured by hemagglutination inhibition assay and neutralizing antibody assays.

RESULTS

Mean change in CD4+ and in CD8+ T-cell frequencies after immunization was 0.01% (P > 0.39) with postimmunization CD4+ frequencies higher than CD8+ frequencies. Children with more previous vaccinations had a higher baseline frequency of CD4+ T-cells (P = 0.0002) but a smaller increase or even a decline from baseline after immunization (P = 0.003). An association between age and change in frequency was not detected. Baseline geometric mean titers (GMTs) and seroprotection rates were significantly higher in older children against A/Panama (neutralizing baseline GMT, P = 0.0488) and A/New Caledonia (hemagglutination inhibition baseline GMT and seroprotection, P < 0.0297). Baseline GMTs against B/Hong Kong were not associated with age or quantity of prior vaccinations.

CONCLUSIONS

These findings suggest that children may plateau in CD4+ T-cell responses to influenza antigens with repeated exposures and that the number of exposures may play a large role in building a memory CD4+ T-cell response to influenza A, perhaps independently from age.

Authors+Show Affiliations

Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305-5208, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17259871

Citation

Zeman, Alenka M., et al. "Humoral and Cellular Immune Responses in Children Given Annual Immunization With Trivalent Inactivated Influenza Vaccine." The Pediatric Infectious Disease Journal, vol. 26, no. 2, 2007, pp. 107-15.
Zeman AM, Holmes TH, Stamatis S, et al. Humoral and cellular immune responses in children given annual immunization with trivalent inactivated influenza vaccine. Pediatr Infect Dis J. 2007;26(2):107-15.
Zeman, A. M., Holmes, T. H., Stamatis, S., Tu, W., He, X. S., Bouvier, N., ... Dekker, C. L. (2007). Humoral and cellular immune responses in children given annual immunization with trivalent inactivated influenza vaccine. The Pediatric Infectious Disease Journal, 26(2), pp. 107-15.
Zeman AM, et al. Humoral and Cellular Immune Responses in Children Given Annual Immunization With Trivalent Inactivated Influenza Vaccine. Pediatr Infect Dis J. 2007;26(2):107-15. PubMed PMID: 17259871.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Humoral and cellular immune responses in children given annual immunization with trivalent inactivated influenza vaccine. AU - Zeman,Alenka M, AU - Holmes,Tyson H, AU - Stamatis,Shaye, AU - Tu,Wenwei, AU - He,Xiao-Song, AU - Bouvier,Nancy, AU - Kemble,George, AU - Greenberg,Harry B, AU - Lewis,David B, AU - Arvin,Ann M, AU - Dekker,Cornelia L, PY - 2007/1/30/pubmed PY - 2007/3/16/medline PY - 2007/1/30/entrez SP - 107 EP - 15 JF - The Pediatric infectious disease journal JO - Pediatr. Infect. Dis. J. VL - 26 IS - 2 N2 - BACKGROUND: There have been no prior reports of the frequency of circulating influenza-specific, interferon gamma-producing memory CD4+ and CD8+ T-cells in healthy children who have received multiple influenza immunizations. METHODS: We evaluated 21 previously immunized children, ages 3 to 9 years, before and 1 month after administration of trivalent inactivated influenza vaccine. Frequencies of influenza-specific CD4+ and CD8+ T-cells stimulated with trivalent inactivated influenza vaccine or A/Panama (H3N2) virus were determined by flow cytometry, and antibody responses to vaccine strains and a drifted H3N2 strain were measured by hemagglutination inhibition assay and neutralizing antibody assays. RESULTS: Mean change in CD4+ and in CD8+ T-cell frequencies after immunization was 0.01% (P > 0.39) with postimmunization CD4+ frequencies higher than CD8+ frequencies. Children with more previous vaccinations had a higher baseline frequency of CD4+ T-cells (P = 0.0002) but a smaller increase or even a decline from baseline after immunization (P = 0.003). An association between age and change in frequency was not detected. Baseline geometric mean titers (GMTs) and seroprotection rates were significantly higher in older children against A/Panama (neutralizing baseline GMT, P = 0.0488) and A/New Caledonia (hemagglutination inhibition baseline GMT and seroprotection, P < 0.0297). Baseline GMTs against B/Hong Kong were not associated with age or quantity of prior vaccinations. CONCLUSIONS: These findings suggest that children may plateau in CD4+ T-cell responses to influenza antigens with repeated exposures and that the number of exposures may play a large role in building a memory CD4+ T-cell response to influenza A, perhaps independently from age. SN - 0891-3668 UR - https://www.unboundmedicine.com/medline/citation/17259871/Humoral_and_cellular_immune_responses_in_children_given_annual_immunization_with_trivalent_inactivated_influenza_vaccine_ L2 - http://Insights.ovid.com/pubmed?pmid=17259871 DB - PRIME DP - Unbound Medicine ER -