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Sequencing microbial copolymers of 3-hydroxybutyric and 3-mercaptoalkanoic acids by NMR, electrospray ionization mass spectrometry, and size exclusion chromatography NMR.
Biomacromolecules. 2007 Mar; 8(3):985-91.B

Abstract

Copolymers of 3-hydroxybutyrate (3HB) and 3-mercaptopropionate (3MP) or 3-mercaptobutyrate (3MB) units and minor amounts of 3-hydroxypropionate (3HP), 3-hydroxyvalerate (3HV), or 3-mercaptovalerate (3MV) were investigated regarding their microstructure by NMR, electrospray ionization mass spectrometry, and size exclusion chromatography NMR. These copolymers were produced by Ralstonia eutropha strain H16 when cells were cultivated in a mineral salts medium with gluconate as a carbon source for growth and 3MP or 3MB as precursor substrates for incorporation of 3-mercaptoalkanoates. Mass spectrometry analysis of partially methanolyzed or pyrolyzed samples proved the presence of true copolymers or terpolymers. (13)C NMR spectroscopy of intact polymer samples, with values of average block length and degree of randomness deviating from a random sequence model, suggested microblock structures; however, composition analysis by (1)H NMR of fractions obtained by size exclusion chromatography showed significant variations with molecular weight, revealing the presence of blends of poly(3HB-co-3MP-co-3HP) or poly(3HB-co-3MB) with poly(3HB). The experimental NMR carbonyl dyad signal intensities were satisfactorily matched by a random sequence model when the presence of poly(3HB) was taken into account.

Authors+Show Affiliations

Impallomeni G
Istituto di Chimica e Tecnologia dei Polimeri, Consiglio Nazionale delle Ricerche, Viale A. Doria 6, 95125 Catania, Italy.
Steinbüchel A
No affiliation info available
Lütke-Eversloh T
No affiliation info available
Barbuzzi T
No affiliation info available
Ballistreri A
No affiliation info available

MeSH

3-Hydroxybutyric AcidBiochemistryChromatographyCupriavidus necatorHydroxybutyratesMagnetic Resonance SpectroscopyMass SpectrometryMethaneModels, ChemicalMolecular ConformationPolyestersPolymersSpectrometry, Mass, Electrospray IonizationSulfhydryl Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17261066

Citation

Impallomeni, Giuseppe, et al. "Sequencing Microbial Copolymers of 3-hydroxybutyric and 3-mercaptoalkanoic Acids By NMR, Electrospray Ionization Mass Spectrometry, and Size Exclusion Chromatography NMR." Biomacromolecules, vol. 8, no. 3, 2007, pp. 985-91.
Impallomeni G, Steinbüchel A, Lütke-Eversloh T, et al. Sequencing microbial copolymers of 3-hydroxybutyric and 3-mercaptoalkanoic acids by NMR, electrospray ionization mass spectrometry, and size exclusion chromatography NMR. Biomacromolecules. 2007;8(3):985-91.
Impallomeni, G., Steinbüchel, A., Lütke-Eversloh, T., Barbuzzi, T., & Ballistreri, A. (2007). Sequencing microbial copolymers of 3-hydroxybutyric and 3-mercaptoalkanoic acids by NMR, electrospray ionization mass spectrometry, and size exclusion chromatography NMR. Biomacromolecules, 8(3), 985-91.
Impallomeni G, et al. Sequencing Microbial Copolymers of 3-hydroxybutyric and 3-mercaptoalkanoic Acids By NMR, Electrospray Ionization Mass Spectrometry, and Size Exclusion Chromatography NMR. Biomacromolecules. 2007;8(3):985-91. PubMed PMID: 17261066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequencing microbial copolymers of 3-hydroxybutyric and 3-mercaptoalkanoic acids by NMR, electrospray ionization mass spectrometry, and size exclusion chromatography NMR. AU - Impallomeni,Giuseppe, AU - Steinbüchel,Alexander, AU - Lütke-Eversloh,Tina, AU - Barbuzzi,Tony, AU - Ballistreri,Alberto, Y1 - 2007/01/30/ PY - 2007/1/31/pubmed PY - 2007/7/12/medline PY - 2007/1/31/entrez SP - 985 EP - 91 JF - Biomacromolecules JO - Biomacromolecules VL - 8 IS - 3 N2 - Copolymers of 3-hydroxybutyrate (3HB) and 3-mercaptopropionate (3MP) or 3-mercaptobutyrate (3MB) units and minor amounts of 3-hydroxypropionate (3HP), 3-hydroxyvalerate (3HV), or 3-mercaptovalerate (3MV) were investigated regarding their microstructure by NMR, electrospray ionization mass spectrometry, and size exclusion chromatography NMR. These copolymers were produced by Ralstonia eutropha strain H16 when cells were cultivated in a mineral salts medium with gluconate as a carbon source for growth and 3MP or 3MB as precursor substrates for incorporation of 3-mercaptoalkanoates. Mass spectrometry analysis of partially methanolyzed or pyrolyzed samples proved the presence of true copolymers or terpolymers. (13)C NMR spectroscopy of intact polymer samples, with values of average block length and degree of randomness deviating from a random sequence model, suggested microblock structures; however, composition analysis by (1)H NMR of fractions obtained by size exclusion chromatography showed significant variations with molecular weight, revealing the presence of blends of poly(3HB-co-3MP-co-3HP) or poly(3HB-co-3MB) with poly(3HB). The experimental NMR carbonyl dyad signal intensities were satisfactorily matched by a random sequence model when the presence of poly(3HB) was taken into account. SN - 1525-7797 UR - https://www.unboundmedicine.com/medline/citation/17261066/Sequencing_microbial_copolymers_of_3_hydroxybutyric_and_3_mercaptoalkanoic_acids_by_NMR_electrospray_ionization_mass_spectrometry_and_size_exclusion_chromatography_NMR_ L2 - https://doi.org/10.1021/bm061014l DB - PRIME DP - Unbound Medicine ER -