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The protective effect of oxytocin on renal ischemia/reperfusion injury in rats.
Regul Pept. 2007 May 03; 140(3):101-8.RP

Abstract

AIM

Oxytocin was previously shown to have anti-inflammatory effects in different inflammation models. The major objective of the present study was to evaluate the protective role of oxytocin (OT) in protecting the kidney against ischemia/reperfusion (I/R) injury.

MATERIALS AND METHODS

Male Wistar albino rats (250-300 g) were unilaterally nephrectomized, and subjected to 45 min of renal pedicle occlusion followed by 6 h of reperfusion. OT (1 mg/kg, ip) or vehicle was administered 15 min prior to ischemia and was repeated immediately before the reperfusion period. At the end of the reperfusion period, rats were decapitated and kidney samples were taken for histological examination or determination of malondialdehyde (MDA), an end product of lipid peroxidation; glutathione (GSH), a key antioxidant; and myeloperoxidase (MPO) activity, an index of tissue neutrophil infiltration. Creatinine and urea concentrations in blood were measured for the evaluation of renal function, while TNF-alpha and lactate dehydrogenase (LDH) levels were determined to evaluate generalized tissue damage. Formation of reactive oxygen species in renal tissue samples was monitored by chemiluminescence technique using luminol and lucigenin probes.

RESULTS

The results revealed that I/R injury increased (p<0.01-0.001) serum urea, creatinine, TNF-alpha and LDH levels, as well as MDA, MPO and reactive oxygen radical levels in the renal tissue, while decreasing renal GSH content. However, alterations in these biochemical and histopathological indices due to I/R injury were attenuated by OT treatment (p<0.05-0.001).

CONCLUSIONS

Since OT administration improved renal function and microscopic damage, along with the alleviation of oxidant tissue responses, it appears that oxytocin protects renal tissue against I/R-induced oxidative damage.

Authors+Show Affiliations

Marmara University, School of Medicine, Department of Pediatric Surgery, Istanbul, Turkey. htugtepe@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17261335

Citation

Tuğtepe, Halil, et al. "The Protective Effect of Oxytocin On Renal Ischemia/reperfusion Injury in Rats." Regulatory Peptides, vol. 140, no. 3, 2007, pp. 101-8.
Tuğtepe H, Sener G, Biyikli NK, et al. The protective effect of oxytocin on renal ischemia/reperfusion injury in rats. Regul Pept. 2007;140(3):101-8.
Tuğtepe, H., Sener, G., Biyikli, N. K., Yüksel, M., Cetinel, S., Gedik, N., & Yeğen, B. C. (2007). The protective effect of oxytocin on renal ischemia/reperfusion injury in rats. Regulatory Peptides, 140(3), 101-8.
Tuğtepe H, et al. The Protective Effect of Oxytocin On Renal Ischemia/reperfusion Injury in Rats. Regul Pept. 2007 May 3;140(3):101-8. PubMed PMID: 17261335.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The protective effect of oxytocin on renal ischemia/reperfusion injury in rats. AU - Tuğtepe,Halil, AU - Sener,Göksel, AU - Biyikli,Neşe Karaaslan, AU - Yüksel,Meral, AU - Cetinel,Sule, AU - Gedik,Nursal, AU - Yeğen,Berrak C, Y1 - 2007/01/29/ PY - 2006/09/07/received PY - 2006/11/09/revised PY - 2006/11/11/accepted PY - 2007/1/31/pubmed PY - 2007/6/30/medline PY - 2007/1/31/entrez SP - 101 EP - 8 JF - Regulatory peptides JO - Regul Pept VL - 140 IS - 3 N2 - AIM: Oxytocin was previously shown to have anti-inflammatory effects in different inflammation models. The major objective of the present study was to evaluate the protective role of oxytocin (OT) in protecting the kidney against ischemia/reperfusion (I/R) injury. MATERIALS AND METHODS: Male Wistar albino rats (250-300 g) were unilaterally nephrectomized, and subjected to 45 min of renal pedicle occlusion followed by 6 h of reperfusion. OT (1 mg/kg, ip) or vehicle was administered 15 min prior to ischemia and was repeated immediately before the reperfusion period. At the end of the reperfusion period, rats were decapitated and kidney samples were taken for histological examination or determination of malondialdehyde (MDA), an end product of lipid peroxidation; glutathione (GSH), a key antioxidant; and myeloperoxidase (MPO) activity, an index of tissue neutrophil infiltration. Creatinine and urea concentrations in blood were measured for the evaluation of renal function, while TNF-alpha and lactate dehydrogenase (LDH) levels were determined to evaluate generalized tissue damage. Formation of reactive oxygen species in renal tissue samples was monitored by chemiluminescence technique using luminol and lucigenin probes. RESULTS: The results revealed that I/R injury increased (p<0.01-0.001) serum urea, creatinine, TNF-alpha and LDH levels, as well as MDA, MPO and reactive oxygen radical levels in the renal tissue, while decreasing renal GSH content. However, alterations in these biochemical and histopathological indices due to I/R injury were attenuated by OT treatment (p<0.05-0.001). CONCLUSIONS: Since OT administration improved renal function and microscopic damage, along with the alleviation of oxidant tissue responses, it appears that oxytocin protects renal tissue against I/R-induced oxidative damage. SN - 0167-0115 UR - https://www.unboundmedicine.com/medline/citation/17261335/The_protective_effect_of_oxytocin_on_renal_ischemia/reperfusion_injury_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-0115(06)00233-3 DB - PRIME DP - Unbound Medicine ER -