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Antimicrobial analysis of different root canal filling pastes used in pediatric dentistry by two experimental methods.
Braz Dent J. 2006; 17(4):317-22.BD

Abstract

The objective of this study was to compare, by two experimental methods, the antimicrobial efficacy of different root canal filling pastes used in pediatric dentistry. The tested materials were: Guedes-Pinto paste (GPP), zinc oxide-eugenol paste (OZEP), calcium hydroxide paste (CHP), chloramphenicol + tetracycline + zinc oxide and eugenol paste (CTZP) and Vitapex. Fiven microbial strains (S. aureus, E. faecalis, P. aeruginosa, B. subtilis and C. albicans) obtained from the American Type Culture Collection were inoculated in Brain Heart Infusion (BHI) and incubated at 37 degrees C for 24 h. For the direct exposure test (DET), 72 paper points were contaminated with the standard microbial suspensions and exposed to the root canal filling pastes for 1, 24, 48 and 72 h. The points were immersed in Letheen Broth (LB), followed by incubation at 37 degrees C for 48 h. An inoculum of 0.1 mL obtained from LB was then transferred to 7 mL of BHI, under identical incubations conditions and the microbial growth was evaluated. The pastes showed activity between 1 and 24 h, depending on the material. For the agar diffusion test (ADT), 30 Petri plates with 20 mL of BHI agar were inoculated with 0.1 mL of the microbial suspension, using sterile swabs that were spread on the medium. Three cavities were made in each agar plate (total = 90) and completely filled with one of the filling root canal pastes. The plates were pre-incubated for 1 h at room temperature and then incubated at 37 degrees C for 24 to 48 h. The inhibition zone around each well was recorded in mm. The complete antimicrobial effect in the direct exposure test was observed after 24 h on all microbial indicators. All root canal filling materials induced the formation of inhibition zones, except for Vitapex (range, 6.0-39.0 mm).

Authors+Show Affiliations

School of Dentistry, Federal University of Goiás, Rua 1, 74115-040 Goiânia, GO, Brazil. lilianguedes@cultura.com.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

17262146

Citation

Amorim, Lilian de Fátima Guedes de, et al. "Antimicrobial Analysis of Different Root Canal Filling Pastes Used in Pediatric Dentistry By Two Experimental Methods." Brazilian Dental Journal, vol. 17, no. 4, 2006, pp. 317-22.
Amorim Lde F, Toledo OA, Estrela CR, et al. Antimicrobial analysis of different root canal filling pastes used in pediatric dentistry by two experimental methods. Braz Dent J. 2006;17(4):317-22.
Amorim, L. d. e. . F., Toledo, O. A., Estrela, C. R., Decurcio, D. d. e. . A., & Estrela, C. (2006). Antimicrobial analysis of different root canal filling pastes used in pediatric dentistry by two experimental methods. Brazilian Dental Journal, 17(4), 317-22.
Amorim Lde F, et al. Antimicrobial Analysis of Different Root Canal Filling Pastes Used in Pediatric Dentistry By Two Experimental Methods. Braz Dent J. 2006;17(4):317-22. PubMed PMID: 17262146.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial analysis of different root canal filling pastes used in pediatric dentistry by two experimental methods. AU - Amorim,Lilian de Fátima Guedes de, AU - Toledo,Orlando Airton de, AU - Estrela,Cyntia Rodrigues de Araújo, AU - Decurcio,Daniel de Almeida, AU - Estrela,Carlos, PY - 2006/05/27/received PY - 2006/05/27/accepted PY - 2007/1/31/pubmed PY - 2007/4/4/medline PY - 2007/1/31/entrez SP - 317 EP - 22 JF - Brazilian dental journal JO - Braz Dent J VL - 17 IS - 4 N2 - The objective of this study was to compare, by two experimental methods, the antimicrobial efficacy of different root canal filling pastes used in pediatric dentistry. The tested materials were: Guedes-Pinto paste (GPP), zinc oxide-eugenol paste (OZEP), calcium hydroxide paste (CHP), chloramphenicol + tetracycline + zinc oxide and eugenol paste (CTZP) and Vitapex. Fiven microbial strains (S. aureus, E. faecalis, P. aeruginosa, B. subtilis and C. albicans) obtained from the American Type Culture Collection were inoculated in Brain Heart Infusion (BHI) and incubated at 37 degrees C for 24 h. For the direct exposure test (DET), 72 paper points were contaminated with the standard microbial suspensions and exposed to the root canal filling pastes for 1, 24, 48 and 72 h. The points were immersed in Letheen Broth (LB), followed by incubation at 37 degrees C for 48 h. An inoculum of 0.1 mL obtained from LB was then transferred to 7 mL of BHI, under identical incubations conditions and the microbial growth was evaluated. The pastes showed activity between 1 and 24 h, depending on the material. For the agar diffusion test (ADT), 30 Petri plates with 20 mL of BHI agar were inoculated with 0.1 mL of the microbial suspension, using sterile swabs that were spread on the medium. Three cavities were made in each agar plate (total = 90) and completely filled with one of the filling root canal pastes. The plates were pre-incubated for 1 h at room temperature and then incubated at 37 degrees C for 24 to 48 h. The inhibition zone around each well was recorded in mm. The complete antimicrobial effect in the direct exposure test was observed after 24 h on all microbial indicators. All root canal filling materials induced the formation of inhibition zones, except for Vitapex (range, 6.0-39.0 mm). SN - 0103-6440 UR - https://www.unboundmedicine.com/medline/citation/17262146/Antimicrobial_analysis_of_different_root_canal_filling_pastes_used_in_pediatric_dentistry_by_two_experimental_methods_ L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402006000400010&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -