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Simultaneous HPLC analysis of biogenic amines, amino acids, and ammonium ion as aminoenone derivatives in wine and beer samples.
J Agric Food Chem. 2007 Feb 07; 55(3):608-13.JA

Abstract

A method has been developed for the simultaneous analysis of biogenic amines, amino acids, and the ammonium ion in wine and beer. Aminoenones formed by the reaction of amino acids, biogenic amines, and the ammonium ion with the derivatization reagent diethyl ethoxymethylenemalonate are separated by HPLC. Reaction takes place in methanolic alkaline medium for 30 min in an ultrasonic bath. Further heating at 70 degrees C for 2 h produces complete degradation of excess derivatization reagent and byproducts. Comparison of the results of ammonium analysis and enzymatic analysis showed a good correlation (r = 0.953). The proposed analytical method has the following advantages: easy derivatization of wines and beers; quantification of 24 amino acids, nine biogenic amines, and the ammonium ion in a single injection; use of the photodiode array detector; complete degradation of excess derivatization reagent during sample preparation; and detection limits below 0.40 mg/L for amino acids and below 0.06 mg/L for biogenic amines.

Authors+Show Affiliations

Instituto de la Vid y el Vino de Castilla-La Mancha, Ctra. Toledo-Albacete s/n, 13700 Tomelloso, Spain. sgomez@ivicam.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17263449

Citation

Gómez-Alonso, Sergio, et al. "Simultaneous HPLC Analysis of Biogenic Amines, Amino Acids, and Ammonium Ion as Aminoenone Derivatives in Wine and Beer Samples." Journal of Agricultural and Food Chemistry, vol. 55, no. 3, 2007, pp. 608-13.
Gómez-Alonso S, Hermosín-Gutiérrez I, García-Romero E. Simultaneous HPLC analysis of biogenic amines, amino acids, and ammonium ion as aminoenone derivatives in wine and beer samples. J Agric Food Chem. 2007;55(3):608-13.
Gómez-Alonso, S., Hermosín-Gutiérrez, I., & García-Romero, E. (2007). Simultaneous HPLC analysis of biogenic amines, amino acids, and ammonium ion as aminoenone derivatives in wine and beer samples. Journal of Agricultural and Food Chemistry, 55(3), 608-13.
Gómez-Alonso S, Hermosín-Gutiérrez I, García-Romero E. Simultaneous HPLC Analysis of Biogenic Amines, Amino Acids, and Ammonium Ion as Aminoenone Derivatives in Wine and Beer Samples. J Agric Food Chem. 2007 Feb 7;55(3):608-13. PubMed PMID: 17263449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous HPLC analysis of biogenic amines, amino acids, and ammonium ion as aminoenone derivatives in wine and beer samples. AU - Gómez-Alonso,Sergio, AU - Hermosín-Gutiérrez,Isidro, AU - García-Romero,Esteban, PY - 2007/2/1/pubmed PY - 2007/5/5/medline PY - 2007/2/1/entrez SP - 608 EP - 13 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 55 IS - 3 N2 - A method has been developed for the simultaneous analysis of biogenic amines, amino acids, and the ammonium ion in wine and beer. Aminoenones formed by the reaction of amino acids, biogenic amines, and the ammonium ion with the derivatization reagent diethyl ethoxymethylenemalonate are separated by HPLC. Reaction takes place in methanolic alkaline medium for 30 min in an ultrasonic bath. Further heating at 70 degrees C for 2 h produces complete degradation of excess derivatization reagent and byproducts. Comparison of the results of ammonium analysis and enzymatic analysis showed a good correlation (r = 0.953). The proposed analytical method has the following advantages: easy derivatization of wines and beers; quantification of 24 amino acids, nine biogenic amines, and the ammonium ion in a single injection; use of the photodiode array detector; complete degradation of excess derivatization reagent during sample preparation; and detection limits below 0.40 mg/L for amino acids and below 0.06 mg/L for biogenic amines. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17263449/Simultaneous_HPLC_analysis_of_biogenic_amines_amino_acids_and_ammonium_ion_as_aminoenone_derivatives_in_wine_and_beer_samples_ L2 - https://doi.org/10.1021/jf062820m DB - PRIME DP - Unbound Medicine ER -