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Amino acid repletion does not decrease muscle protein catabolism during hemodialysis.
Am J Physiol Endocrinol Metab 2007; 292(6):E1534-42AJ

Abstract

Intradialytic protein catabolism is attributed to loss of amino acids in the dialysate. We investigated the effect of amino acid infusion during hemodialysis (HD) on muscle protein turnover and amino acid transport kinetics by using stable isotopes of phenylalanine, leucine, and lysine in eight patients with end-stage renal disease (ESRD). Subjects were studied at baseline (pre-HD), 2 h of HD without amino acid infusion (HD-O), and 2 h of HD with amino acid infusion (HD+AA). Amino acid depletion during HD-O augmented the outward transport of amino acids from muscle into the vein. Increased delivery of amino acids to the leg during HD+AA facilitated the transport of amino acids from the artery into the intracellular compartment. Increase in muscle protein breakdown was more than the increase in synthesis during HD-O (46.7 vs. 22.3%, P < 0.001). Net balance (nmol.min(-1).100 ml (-1)) was more negative during HD-O compared with pre-HD (-33.7 +/- 1.5 vs. -6.0 +/- 2.3, P < 0.001). Despite an abundant supply of amino acids, the net balance (-16.9 +/- 1.8) did not switch from net release to net uptake. HD+AA induced a proportional increase in muscle protein synthesis and catabolism. Branched chain amino acid catabolism increased significantly from baseline during HD-O and did not decrease during HD+AA. Protein synthesis efficiency, the fraction of amino acid in the intracellular pool that is utilized for muscle protein synthesis decreased from 42.1% pre-HD to 33.7 and 32.6% during HD-O and HD+AA, respectively (P < 0.01). Thus amino acid repletion during HD increased muscle protein synthesis but did not decrease muscle protein breakdown.

Authors+Show Affiliations

Division of Nephrology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131-5271, USA. draj@salud.unm.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Controlled Clinical Trial
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17264222

Citation

Raj, Dominic S C., et al. "Amino Acid Repletion Does Not Decrease Muscle Protein Catabolism During Hemodialysis." American Journal of Physiology. Endocrinology and Metabolism, vol. 292, no. 6, 2007, pp. E1534-42.
Raj DS, Adeniyi O, Dominic EA, et al. Amino acid repletion does not decrease muscle protein catabolism during hemodialysis. Am J Physiol Endocrinol Metab. 2007;292(6):E1534-42.
Raj, D. S., Adeniyi, O., Dominic, E. A., Boivin, M. A., McClelland, S., Tzamaloukas, A. H., ... Ferrando, A. (2007). Amino acid repletion does not decrease muscle protein catabolism during hemodialysis. American Journal of Physiology. Endocrinology and Metabolism, 292(6), pp. E1534-42.
Raj DS, et al. Amino Acid Repletion Does Not Decrease Muscle Protein Catabolism During Hemodialysis. Am J Physiol Endocrinol Metab. 2007;292(6):E1534-42. PubMed PMID: 17264222.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amino acid repletion does not decrease muscle protein catabolism during hemodialysis. AU - Raj,Dominic S C, AU - Adeniyi,Oladipo, AU - Dominic,Elizabeth A, AU - Boivin,Michel A, AU - McClelland,Sandra, AU - Tzamaloukas,Antonios H, AU - Morgan,Nancy, AU - Gonzales,Lawrence, AU - Wolfe,Robert, AU - Ferrando,Arny, Y1 - 2007/01/30/ PY - 2007/2/1/pubmed PY - 2007/7/31/medline PY - 2007/2/1/entrez SP - E1534 EP - 42 JF - American journal of physiology. Endocrinology and metabolism JO - Am. J. Physiol. Endocrinol. Metab. VL - 292 IS - 6 N2 - Intradialytic protein catabolism is attributed to loss of amino acids in the dialysate. We investigated the effect of amino acid infusion during hemodialysis (HD) on muscle protein turnover and amino acid transport kinetics by using stable isotopes of phenylalanine, leucine, and lysine in eight patients with end-stage renal disease (ESRD). Subjects were studied at baseline (pre-HD), 2 h of HD without amino acid infusion (HD-O), and 2 h of HD with amino acid infusion (HD+AA). Amino acid depletion during HD-O augmented the outward transport of amino acids from muscle into the vein. Increased delivery of amino acids to the leg during HD+AA facilitated the transport of amino acids from the artery into the intracellular compartment. Increase in muscle protein breakdown was more than the increase in synthesis during HD-O (46.7 vs. 22.3%, P < 0.001). Net balance (nmol.min(-1).100 ml (-1)) was more negative during HD-O compared with pre-HD (-33.7 +/- 1.5 vs. -6.0 +/- 2.3, P < 0.001). Despite an abundant supply of amino acids, the net balance (-16.9 +/- 1.8) did not switch from net release to net uptake. HD+AA induced a proportional increase in muscle protein synthesis and catabolism. Branched chain amino acid catabolism increased significantly from baseline during HD-O and did not decrease during HD+AA. Protein synthesis efficiency, the fraction of amino acid in the intracellular pool that is utilized for muscle protein synthesis decreased from 42.1% pre-HD to 33.7 and 32.6% during HD-O and HD+AA, respectively (P < 0.01). Thus amino acid repletion during HD increased muscle protein synthesis but did not decrease muscle protein breakdown. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/17264222/Amino_acid_repletion_does_not_decrease_muscle_protein_catabolism_during_hemodialysis_ L2 - http://www.physiology.org/doi/full/10.1152/ajpendo.00599.2006?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -