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Ameliorative effect of vitamins (alpha-tocopherol and ascorbic acid) on PCB (Aroclor 1254) induced oxidative stress in rat epididymal sperm.
Reprod Toxicol. 2007 Feb; 23(2):239-45.RT

Abstract

The aim of this study was to investigate the possible protective role of vitamins on PCB (Aroclor 1254)-induced spermiotoxicity using qualitative, quantitative and biochemical approaches. Adult male albino rats of Wistar strain were randomly divided into four groups, each group consists of six animals. The control group received corn oil, the second group of rats were administered Aroclor 1254 at a dose of 2 mg/kg bw/day intraperitoneally for 30 days. The third group of rats were treated with Aroclor 1254 along with alpha-tocopherol (50 mg/kg of bw/day) for 30 days, while the fourth group of rats were treated with Aroclor 1254 along with ascorbic acid (100 mg/kg bw/day) orally for 30 days. Twenty-four hours after the last treatment, control and experimental animals were killed by decapitation. Sperm was collected from the cauda epididymal region and its count and motility were detected. Sperm was sonicated and used for the estimation of reactive oxygen species (ROS) [hydroxyl radical (HO(*)) and hydrogen peroxide (H(2)O(2))], non-enzymic antioxidants [alpha-tocopherol, ascorbic acid and reduced glutathione (GSH)], activity of enzymic antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) glutathione reductase (GR) and glutathione-S-transferase (GST)] and lipid peroxidation (LPO). The result of this experiment shows that PCB significantly decreases the level of alpha-tocopherol, ascorbic acid and GSH and the activities of SOD, CAT, GPx, GR and GST with elevated levels of ROS and LPO. In addition, decreased epididymal sperm motility and count were observed. Simultaneous supplementation with alpha-tocopherol and ascorbic acid restored these parameters to that of normal range. In conclusion, alpha-tocopherol and ascorbic acid exhibited protective effect on sperm by inhibiting PCB-induced ROS generation.

Authors+Show Affiliations

Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600113, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17267175

Citation

Krishnamoorthy, G, et al. "Ameliorative Effect of Vitamins (alpha-tocopherol and Ascorbic Acid) On PCB (Aroclor 1254) Induced Oxidative Stress in Rat Epididymal Sperm." Reproductive Toxicology (Elmsford, N.Y.), vol. 23, no. 2, 2007, pp. 239-45.
Krishnamoorthy G, Venkataraman P, Arunkumar A, et al. Ameliorative effect of vitamins (alpha-tocopherol and ascorbic acid) on PCB (Aroclor 1254) induced oxidative stress in rat epididymal sperm. Reprod Toxicol. 2007;23(2):239-45.
Krishnamoorthy, G., Venkataraman, P., Arunkumar, A., Vignesh, R. C., Aruldhas, M. M., & Arunakaran, J. (2007). Ameliorative effect of vitamins (alpha-tocopherol and ascorbic acid) on PCB (Aroclor 1254) induced oxidative stress in rat epididymal sperm. Reproductive Toxicology (Elmsford, N.Y.), 23(2), 239-45.
Krishnamoorthy G, et al. Ameliorative Effect of Vitamins (alpha-tocopherol and Ascorbic Acid) On PCB (Aroclor 1254) Induced Oxidative Stress in Rat Epididymal Sperm. Reprod Toxicol. 2007;23(2):239-45. PubMed PMID: 17267175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ameliorative effect of vitamins (alpha-tocopherol and ascorbic acid) on PCB (Aroclor 1254) induced oxidative stress in rat epididymal sperm. AU - Krishnamoorthy,G, AU - Venkataraman,P, AU - Arunkumar,A, AU - Vignesh,R C, AU - Aruldhas,M M, AU - Arunakaran,J, Y1 - 2006/12/22/ PY - 2006/03/09/received PY - 2006/11/27/revised PY - 2006/12/15/accepted PY - 2007/2/3/pubmed PY - 2007/4/18/medline PY - 2007/2/3/entrez SP - 239 EP - 45 JF - Reproductive toxicology (Elmsford, N.Y.) JO - Reprod Toxicol VL - 23 IS - 2 N2 - The aim of this study was to investigate the possible protective role of vitamins on PCB (Aroclor 1254)-induced spermiotoxicity using qualitative, quantitative and biochemical approaches. Adult male albino rats of Wistar strain were randomly divided into four groups, each group consists of six animals. The control group received corn oil, the second group of rats were administered Aroclor 1254 at a dose of 2 mg/kg bw/day intraperitoneally for 30 days. The third group of rats were treated with Aroclor 1254 along with alpha-tocopherol (50 mg/kg of bw/day) for 30 days, while the fourth group of rats were treated with Aroclor 1254 along with ascorbic acid (100 mg/kg bw/day) orally for 30 days. Twenty-four hours after the last treatment, control and experimental animals were killed by decapitation. Sperm was collected from the cauda epididymal region and its count and motility were detected. Sperm was sonicated and used for the estimation of reactive oxygen species (ROS) [hydroxyl radical (HO(*)) and hydrogen peroxide (H(2)O(2))], non-enzymic antioxidants [alpha-tocopherol, ascorbic acid and reduced glutathione (GSH)], activity of enzymic antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) glutathione reductase (GR) and glutathione-S-transferase (GST)] and lipid peroxidation (LPO). The result of this experiment shows that PCB significantly decreases the level of alpha-tocopherol, ascorbic acid and GSH and the activities of SOD, CAT, GPx, GR and GST with elevated levels of ROS and LPO. In addition, decreased epididymal sperm motility and count were observed. Simultaneous supplementation with alpha-tocopherol and ascorbic acid restored these parameters to that of normal range. In conclusion, alpha-tocopherol and ascorbic acid exhibited protective effect on sperm by inhibiting PCB-induced ROS generation. SN - 0890-6238 UR - https://www.unboundmedicine.com/medline/citation/17267175/Ameliorative_effect_of_vitamins__alpha_tocopherol_and_ascorbic_acid__on_PCB__Aroclor_1254__induced_oxidative_stress_in_rat_epididymal_sperm_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0890-6238(06)00300-5 DB - PRIME DP - Unbound Medicine ER -