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Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum.
J Clin Microbiol. 2007 Apr; 45(4):1200-4.JC

Abstract

A rapid two-step DNA extraction method and a multiplex PCR for the detection of dermatophytes in general and Trichophyton rubrum specifically were developed and evaluated with DNA extracted from pure cultures and from clinically diseased nails. DNA from the following dermatophytes was used: Epidermophyton floccosum, Microsporum audouinii, Microsporum canis, Microsporum gypseum, Microsporum nanum, Trichophyton mentagrophytes, Trichophyton rubrum, Trichophyton schoenleinii, Trichophyton soudanense, Trichophyton terrestre, Trichophyton tonsurans, Trichophyton verrucosum, and Trichophyton violaceum. Human DNA and DNA from the following nondermatophyte fungi were included as controls: Alternaria, Aspergillus niger, Candida albicans, Candida glabrata, Candida krusei, Malassezia furfur, Saccharomyces cerevisiae, and Scopulariopsis brevicaulis. A total of 118 nail samples received for routine microscopy and culture for dermatophytes were subsequently tested by the two PCRs separately and in a multiplex format. Using DNA extracted from pure cultures and the pan-dermatophyte PCR, the T. rubrum-specific PCR sequentially and in a multiplex format correctly detected all dermatophytes and additionally correctly identified T. rubrum. Comparison of the traditional diagnostic evaluation (microscopy and culture) of nail samples with PCR on DNA directly extracted from the nails showed excellent agreement between PCR and microscopy, but the number of samples with dermatophyte species identification was increased considerably from 22.9% to 41.5%, mainly due to the identification of T. rubrum by PCR in microscopy-positive but culture-negative samples. In conclusion, this 5-hour diagnostic test was shown to increase not only the speed but also the sensitivity of investigation for nail dermatophytosis.

Authors+Show Affiliations

Unit of Mycology and Parasitology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

17267633

Citation

Brillowska-Dabrowska, Anna, et al. "Five-hour Diagnosis of Dermatophyte Nail Infections With Specific Detection of Trichophyton Rubrum." Journal of Clinical Microbiology, vol. 45, no. 4, 2007, pp. 1200-4.
Brillowska-Dabrowska A, Saunte DM, Arendrup MC. Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol. 2007;45(4):1200-4.
Brillowska-Dabrowska, A., Saunte, D. M., & Arendrup, M. C. (2007). Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. Journal of Clinical Microbiology, 45(4), 1200-4.
Brillowska-Dabrowska A, Saunte DM, Arendrup MC. Five-hour Diagnosis of Dermatophyte Nail Infections With Specific Detection of Trichophyton Rubrum. J Clin Microbiol. 2007;45(4):1200-4. PubMed PMID: 17267633.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. AU - Brillowska-Dabrowska,Anna, AU - Saunte,Ditte Marie, AU - Arendrup,Maiken Cavling, Y1 - 2007/01/31/ PY - 2007/2/3/pubmed PY - 2007/10/12/medline PY - 2007/2/3/entrez SP - 1200 EP - 4 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 45 IS - 4 N2 - A rapid two-step DNA extraction method and a multiplex PCR for the detection of dermatophytes in general and Trichophyton rubrum specifically were developed and evaluated with DNA extracted from pure cultures and from clinically diseased nails. DNA from the following dermatophytes was used: Epidermophyton floccosum, Microsporum audouinii, Microsporum canis, Microsporum gypseum, Microsporum nanum, Trichophyton mentagrophytes, Trichophyton rubrum, Trichophyton schoenleinii, Trichophyton soudanense, Trichophyton terrestre, Trichophyton tonsurans, Trichophyton verrucosum, and Trichophyton violaceum. Human DNA and DNA from the following nondermatophyte fungi were included as controls: Alternaria, Aspergillus niger, Candida albicans, Candida glabrata, Candida krusei, Malassezia furfur, Saccharomyces cerevisiae, and Scopulariopsis brevicaulis. A total of 118 nail samples received for routine microscopy and culture for dermatophytes were subsequently tested by the two PCRs separately and in a multiplex format. Using DNA extracted from pure cultures and the pan-dermatophyte PCR, the T. rubrum-specific PCR sequentially and in a multiplex format correctly detected all dermatophytes and additionally correctly identified T. rubrum. Comparison of the traditional diagnostic evaluation (microscopy and culture) of nail samples with PCR on DNA directly extracted from the nails showed excellent agreement between PCR and microscopy, but the number of samples with dermatophyte species identification was increased considerably from 22.9% to 41.5%, mainly due to the identification of T. rubrum by PCR in microscopy-positive but culture-negative samples. In conclusion, this 5-hour diagnostic test was shown to increase not only the speed but also the sensitivity of investigation for nail dermatophytosis. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/17267633/Five_hour_diagnosis_of_dermatophyte_nail_infections_with_specific_detection_of_Trichophyton_rubrum_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=17267633 DB - PRIME DP - Unbound Medicine ER -