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Molecular and biological characterization of a naturally occurring recombinant subgroup B avian leukosis virus with a subgroup J-like long terminal repeat.
Avian Dis. 2006 Dec; 50(4):572-8.AD

Abstract

Infection of broiler chickens with subgroup J avian leukosis virus (ALV) results in the induction of myeloid tumors. However, although egg-type chickens are susceptible to infection with ALV-J, the tumor incidence is very low, and on rare occasions the tumors observed are of the myeloid lineage. We recently described the isolation of an ALV (AF115-4) from commercial egg-type chickens suffering from myeloid leukosis. AF115-4 was initially identified as an ALV-J isolate based on PCR analysis of the long terminal repeat (LTR). However, further characterization of the viral envelope indicated that the virus is recombinant with subgroups B envelope and J LTR. Here we further characterize this recombinant virus at both the molecular and biological levels. We show that the AF115-4 isolate expresses a recombinant envelope glycoprotein encoded by a subgroup B gp85 region and a subgroup E gp37 region. The host range ofAF115-4 was analyzed using cells resistant to infection by subgroups A/B, J, or E; this shows that no ALV-J was present in the isolates obtained from the affected chickens. Additional antigenic characterization of AF115-4 using chicken sera specific for subgroups B or J indicated that no ALV-J was present in the samples examined. Inoculation of AF 115-4 into ALV-susceptible 1515 X 71 chickens resulted in the induction of lymphoid leukosis but not the expected myeloid leukosis affecting the commercial chickens. These results suggest that differences in the genetic makeup of the chickens from which AF115-4 was isolated and the line 1515 X 71 used in the present experiments may be responsible for the observed differences in pathogenicity. In addition, the results suggest that ALV-J continues to evolve by recombination, generating new viruses with different pathological properties.

Authors+Show Affiliations

Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17274296

Citation

Lupiani, Blanca, et al. "Molecular and Biological Characterization of a Naturally Occurring Recombinant Subgroup B Avian Leukosis Virus With a Subgroup J-like Long Terminal Repeat." Avian Diseases, vol. 50, no. 4, 2006, pp. 572-8.
Lupiani B, Pandiri AR, Mays J, et al. Molecular and biological characterization of a naturally occurring recombinant subgroup B avian leukosis virus with a subgroup J-like long terminal repeat. Avian Dis. 2006;50(4):572-8.
Lupiani, B., Pandiri, A. R., Mays, J., Hunt, H. D., & Fadly, A. M. (2006). Molecular and biological characterization of a naturally occurring recombinant subgroup B avian leukosis virus with a subgroup J-like long terminal repeat. Avian Diseases, 50(4), 572-8.
Lupiani B, et al. Molecular and Biological Characterization of a Naturally Occurring Recombinant Subgroup B Avian Leukosis Virus With a Subgroup J-like Long Terminal Repeat. Avian Dis. 2006;50(4):572-8. PubMed PMID: 17274296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and biological characterization of a naturally occurring recombinant subgroup B avian leukosis virus with a subgroup J-like long terminal repeat. AU - Lupiani,Blanca, AU - Pandiri,Arun R, AU - Mays,Jody, AU - Hunt,Henry D, AU - Fadly,Aly M, PY - 2007/2/6/pubmed PY - 2007/3/3/medline PY - 2007/2/6/entrez SP - 572 EP - 8 JF - Avian diseases JO - Avian Dis VL - 50 IS - 4 N2 - Infection of broiler chickens with subgroup J avian leukosis virus (ALV) results in the induction of myeloid tumors. However, although egg-type chickens are susceptible to infection with ALV-J, the tumor incidence is very low, and on rare occasions the tumors observed are of the myeloid lineage. We recently described the isolation of an ALV (AF115-4) from commercial egg-type chickens suffering from myeloid leukosis. AF115-4 was initially identified as an ALV-J isolate based on PCR analysis of the long terminal repeat (LTR). However, further characterization of the viral envelope indicated that the virus is recombinant with subgroups B envelope and J LTR. Here we further characterize this recombinant virus at both the molecular and biological levels. We show that the AF115-4 isolate expresses a recombinant envelope glycoprotein encoded by a subgroup B gp85 region and a subgroup E gp37 region. The host range ofAF115-4 was analyzed using cells resistant to infection by subgroups A/B, J, or E; this shows that no ALV-J was present in the isolates obtained from the affected chickens. Additional antigenic characterization of AF115-4 using chicken sera specific for subgroups B or J indicated that no ALV-J was present in the samples examined. Inoculation of AF 115-4 into ALV-susceptible 1515 X 71 chickens resulted in the induction of lymphoid leukosis but not the expected myeloid leukosis affecting the commercial chickens. These results suggest that differences in the genetic makeup of the chickens from which AF115-4 was isolated and the line 1515 X 71 used in the present experiments may be responsible for the observed differences in pathogenicity. In addition, the results suggest that ALV-J continues to evolve by recombination, generating new viruses with different pathological properties. SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/17274296/Molecular_and_biological_characterization_of_a_naturally_occurring_recombinant_subgroup_B_avian_leukosis_virus_with_a_subgroup_J_like_long_terminal_repeat_ L2 - https://doi.org/10.1637/7656-053006R.1 DB - PRIME DP - Unbound Medicine ER -