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PT with heat-processed apple peel extract to detect LTP hypersensitivity.
Eur Ann Allergy Clin Immunol 2006; 38(10):351-4EA

Abstract

BACKGROUND

Allergen-resolved diagnosis of food allergy may be essential in the clinical practice, particularly in patients allergic to foods that may contain both labile and stable allergens. However, presently available diagnostic tests are not useful in this sense.

OBJECTIVE

To assess the clinical usefulness of SPT with a heat-processed apple peel extract as an easily available means to detect hypersensitivity to lipid transfer protein (LTP), an extremely stable and potentially hazardous apple allergen.

METHODS

Raw and heat-processed (100 degrees C x 10 min) apple peel extract (100 microg/ml) were used to carry out SPT in 23 patients with apple-allergy, 15 of which considered as probably sensitized to labile allergens (Maid 1, Mal d 4) and 8 to stable allergens (Mal d 3, LTP), respectively on the basis of the presence/absence of IgE reactivity to birch pollen. IgE reactivity to the heat-processed apple peel extract was further analyzed by immunoblot.

RESULTS

Altogether SPT with raw apple extract scored positive in 20/23 (87%) patients, including 12/15 patients considered as probably sensitized to labile allergens and 8/8 patients considered as probably sensitized to LTP. In contrast, the heat-processed apple extract induced a wheal-and-flare reaction only in the 8 (100%) presumptive LTP reactors. Immunoblot analysis showed IgE reactivity to a 10 kDa protein (LTP) in heat-processed apple extract.

CONCLUSION

The heat-resistance of stable apple allergens like LTP can be usefully exploited to prepare extracts for allergen-resolved diagnosis in-vivo.

Authors+Show Affiliations

Ambulatorio di Allergologia, Clinica San Carlo, Paderno Dugnano, MI, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17274519

Citation

Asero, R, et al. "PT With Heat-processed Apple Peel Extract to Detect LTP Hypersensitivity." European Annals of Allergy and Clinical Immunology, vol. 38, no. 10, 2006, pp. 351-4.
Asero R, Mistrello G, Roncarolo D, et al. PT with heat-processed apple peel extract to detect LTP hypersensitivity. Eur Ann Allergy Clin Immunol. 2006;38(10):351-4.
Asero, R., Mistrello, G., Roncarolo, D., & Amato, S. (2006). PT with heat-processed apple peel extract to detect LTP hypersensitivity. European Annals of Allergy and Clinical Immunology, 38(10), pp. 351-4.
Asero R, et al. PT With Heat-processed Apple Peel Extract to Detect LTP Hypersensitivity. Eur Ann Allergy Clin Immunol. 2006;38(10):351-4. PubMed PMID: 17274519.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PT with heat-processed apple peel extract to detect LTP hypersensitivity. AU - Asero,R, AU - Mistrello,G, AU - Roncarolo,D, AU - Amato,S, PY - 2007/2/6/pubmed PY - 2007/3/3/medline PY - 2007/2/6/entrez SP - 351 EP - 4 JF - European annals of allergy and clinical immunology JO - Eur Ann Allergy Clin Immunol VL - 38 IS - 10 N2 - BACKGROUND: Allergen-resolved diagnosis of food allergy may be essential in the clinical practice, particularly in patients allergic to foods that may contain both labile and stable allergens. However, presently available diagnostic tests are not useful in this sense. OBJECTIVE: To assess the clinical usefulness of SPT with a heat-processed apple peel extract as an easily available means to detect hypersensitivity to lipid transfer protein (LTP), an extremely stable and potentially hazardous apple allergen. METHODS: Raw and heat-processed (100 degrees C x 10 min) apple peel extract (100 microg/ml) were used to carry out SPT in 23 patients with apple-allergy, 15 of which considered as probably sensitized to labile allergens (Maid 1, Mal d 4) and 8 to stable allergens (Mal d 3, LTP), respectively on the basis of the presence/absence of IgE reactivity to birch pollen. IgE reactivity to the heat-processed apple peel extract was further analyzed by immunoblot. RESULTS: Altogether SPT with raw apple extract scored positive in 20/23 (87%) patients, including 12/15 patients considered as probably sensitized to labile allergens and 8/8 patients considered as probably sensitized to LTP. In contrast, the heat-processed apple extract induced a wheal-and-flare reaction only in the 8 (100%) presumptive LTP reactors. Immunoblot analysis showed IgE reactivity to a 10 kDa protein (LTP) in heat-processed apple extract. CONCLUSION: The heat-resistance of stable apple allergens like LTP can be usefully exploited to prepare extracts for allergen-resolved diagnosis in-vivo. SN - 1764-1489 UR - https://www.unboundmedicine.com/medline/citation/17274519/PT_with_heat_processed_apple_peel_extract_to_detect_LTP_hypersensitivity_ L2 - https://medlineplus.gov/foodallergy.html DB - PRIME DP - Unbound Medicine ER -