Tags

Type your tag names separated by a space and hit enter

Characterization of heart size and blood pressure in the vitamin D receptor knockout mouse.
J Steroid Biochem Mol Biol 2007; 103(3-5):521-4JS

Abstract

Our previous studies showed vitamin D deficiency results in increased cardiac contractility, hypertrophy and fibrosis and has profound effects on heart proteomics, structure and function in rat. In this study we found that the heart in vitamin D receptor knockout (VDR-KO) mice is hypertrophied. Six homozygous VDR knockout (-/-), six wild type (+/+) and six heterozygous (+/-) mice were fed a diet containing 2% Ca, 1.25% P and 20% lactose to maintain normal blood calcium and phosphate levels for 12 months. Tail-cuff blood pressure was performed on all mice. Blood pressure determinations showed no differences in systolic or mean blood pressure in WT (+/+), KO (-/-) or HETERO (+/-) mice at 3 and 6 months. However, decreased systolic BP in the KO mice relative to WT at 9 months of age was observed. ECG analysis showed no significant differences in the intact KO, HETERO or WT mice. The mice were killed at 12 months. Heart weight/body weight ratio was 41% (P<.003) greater in the KO mice versus WT and HETERO was 19% (P<.05) increased versus WT. Other VDR-KO tissues did not display hypertrophy. Cross sectional and longitudinal analysis of the heart myofibrils showed highly significant cellular hypertrophy in VDR-KO mice. Trichrome staining of heart tissue showed marked increase in fibrotic lesions in the KO mice. Analysis of plasma renin activity, angiotensin II (AII) and aldosterone levels showed elevated but not significantly different renin activity in KO versus WT and no significant differences in AII or aldosterone levels. Our data do not support the concept that the renin-angiotensin system or hypertension are the factors that elicit these changes. Data presented here reveal that ablation of the VDR signaling system results in profound changes in heart structure. We propose that calcitriol acts directly on the heart as a tranquilizer by blunting cardiomyocyte hypertrophy.

Authors+Show Affiliations

Department of Pharmacology, University of Michigan, School of Medicine, 1301 MSRB III, 1150 West Medical Center Drive, Ann Arbor, MI 48109-0632, USA. robsim@umich.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17275289

Citation

Simpson, Robert U., et al. "Characterization of Heart Size and Blood Pressure in the Vitamin D Receptor Knockout Mouse." The Journal of Steroid Biochemistry and Molecular Biology, vol. 103, no. 3-5, 2007, pp. 521-4.
Simpson RU, Hershey SH, Nibbelink KA. Characterization of heart size and blood pressure in the vitamin D receptor knockout mouse. J Steroid Biochem Mol Biol. 2007;103(3-5):521-4.
Simpson, R. U., Hershey, S. H., & Nibbelink, K. A. (2007). Characterization of heart size and blood pressure in the vitamin D receptor knockout mouse. The Journal of Steroid Biochemistry and Molecular Biology, 103(3-5), pp. 521-4.
Simpson RU, Hershey SH, Nibbelink KA. Characterization of Heart Size and Blood Pressure in the Vitamin D Receptor Knockout Mouse. J Steroid Biochem Mol Biol. 2007;103(3-5):521-4. PubMed PMID: 17275289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of heart size and blood pressure in the vitamin D receptor knockout mouse. AU - Simpson,Robert U, AU - Hershey,Steven H, AU - Nibbelink,Karl A, Y1 - 2006/12/23/ PY - 2007/2/6/pubmed PY - 2007/5/12/medline PY - 2007/2/6/entrez SP - 521 EP - 4 JF - The Journal of steroid biochemistry and molecular biology JO - J. Steroid Biochem. Mol. Biol. VL - 103 IS - 3-5 N2 - Our previous studies showed vitamin D deficiency results in increased cardiac contractility, hypertrophy and fibrosis and has profound effects on heart proteomics, structure and function in rat. In this study we found that the heart in vitamin D receptor knockout (VDR-KO) mice is hypertrophied. Six homozygous VDR knockout (-/-), six wild type (+/+) and six heterozygous (+/-) mice were fed a diet containing 2% Ca, 1.25% P and 20% lactose to maintain normal blood calcium and phosphate levels for 12 months. Tail-cuff blood pressure was performed on all mice. Blood pressure determinations showed no differences in systolic or mean blood pressure in WT (+/+), KO (-/-) or HETERO (+/-) mice at 3 and 6 months. However, decreased systolic BP in the KO mice relative to WT at 9 months of age was observed. ECG analysis showed no significant differences in the intact KO, HETERO or WT mice. The mice were killed at 12 months. Heart weight/body weight ratio was 41% (P<.003) greater in the KO mice versus WT and HETERO was 19% (P<.05) increased versus WT. Other VDR-KO tissues did not display hypertrophy. Cross sectional and longitudinal analysis of the heart myofibrils showed highly significant cellular hypertrophy in VDR-KO mice. Trichrome staining of heart tissue showed marked increase in fibrotic lesions in the KO mice. Analysis of plasma renin activity, angiotensin II (AII) and aldosterone levels showed elevated but not significantly different renin activity in KO versus WT and no significant differences in AII or aldosterone levels. Our data do not support the concept that the renin-angiotensin system or hypertension are the factors that elicit these changes. Data presented here reveal that ablation of the VDR signaling system results in profound changes in heart structure. We propose that calcitriol acts directly on the heart as a tranquilizer by blunting cardiomyocyte hypertrophy. SN - 0960-0760 UR - https://www.unboundmedicine.com/medline/citation/17275289/Characterization_of_heart_size_and_blood_pressure_in_the_vitamin_D_receptor_knockout_mouse_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-0760(06)00464-X DB - PRIME DP - Unbound Medicine ER -