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Induction and activity of oxidative stress-related proteins during waterborne Cd/Zn-exposure in brown trout (Salmo trutta).
Chemosphere. 2007 May; 67(11):2241-9.C

Abstract

We studied how transcript levels of metallothionein (MT), Cu/Zn-superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) as well as functional protein levels of MT, SOD and CAT in brown trout tissues changed during a 15-days waterborne exposure to Cd and Zn. Trout from a river with low levels of metals (the Stribekken River) was transferred to a river with high levels of Cd and Zn (the Naustebekken River) and exposed up to 15 days. The aim of this transfer experiment was to investigate how exposure to Cd and Zn induced transcription and activities of central antioxidant enzymes and proteins in an environmental setting. Significant uptake of both Cd and Zn was observed in gills during the 15 days exposure, and Cd levels was found to correlate significantly with transcript levels of MT-A, SOD, GPx and GR. Gill concentrations of Zn did not correlate significantly with the transcript levels of the stress genes studied, but Zn might have triggered transcription of proteins which dealt with subsequent accumulation of Cd. SOD and CAT activities increased in gills after transfer, but MT protein levels decreased. In liver, SOD activity and MT protein levels increased, while in kidney only MT protein concentrations were elevated after transfer. There was a general lack of consistency between mRNA transcription and enzyme activities, indicating that these proteins and enzymes are not solely under transcriptional control.

Authors+Show Affiliations

Norwegian University of Science and Technology (NTNU), Department of Biology, Høgskoleringen 5, N-7491 Trondheim, Norway. bjorn.h.hansen@sintef.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17276485

Citation

Hansen, Bjørn Henrik, et al. "Induction and Activity of Oxidative Stress-related Proteins During Waterborne Cd/Zn-exposure in Brown Trout (Salmo Trutta)." Chemosphere, vol. 67, no. 11, 2007, pp. 2241-9.
Hansen BH, Rømma S, Garmo ØA, et al. Induction and activity of oxidative stress-related proteins during waterborne Cd/Zn-exposure in brown trout (Salmo trutta). Chemosphere. 2007;67(11):2241-9.
Hansen, B. H., Rømma, S., Garmo, Ø. A., Pedersen, S. A., Olsvik, P. A., & Andersen, R. A. (2007). Induction and activity of oxidative stress-related proteins during waterborne Cd/Zn-exposure in brown trout (Salmo trutta). Chemosphere, 67(11), 2241-9.
Hansen BH, et al. Induction and Activity of Oxidative Stress-related Proteins During Waterborne Cd/Zn-exposure in Brown Trout (Salmo Trutta). Chemosphere. 2007;67(11):2241-9. PubMed PMID: 17276485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction and activity of oxidative stress-related proteins during waterborne Cd/Zn-exposure in brown trout (Salmo trutta). AU - Hansen,Bjørn Henrik, AU - Rømma,Svein, AU - Garmo,Øyvind Aaberg, AU - Pedersen,Sindre Andre, AU - Olsvik,Pål Asgeir, AU - Andersen,Rolf Arvid, Y1 - 2007/02/05/ PY - 2006/05/24/received PY - 2006/12/06/revised PY - 2006/12/07/accepted PY - 2007/2/6/pubmed PY - 2007/6/15/medline PY - 2007/2/6/entrez SP - 2241 EP - 9 JF - Chemosphere JO - Chemosphere VL - 67 IS - 11 N2 - We studied how transcript levels of metallothionein (MT), Cu/Zn-superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) as well as functional protein levels of MT, SOD and CAT in brown trout tissues changed during a 15-days waterborne exposure to Cd and Zn. Trout from a river with low levels of metals (the Stribekken River) was transferred to a river with high levels of Cd and Zn (the Naustebekken River) and exposed up to 15 days. The aim of this transfer experiment was to investigate how exposure to Cd and Zn induced transcription and activities of central antioxidant enzymes and proteins in an environmental setting. Significant uptake of both Cd and Zn was observed in gills during the 15 days exposure, and Cd levels was found to correlate significantly with transcript levels of MT-A, SOD, GPx and GR. Gill concentrations of Zn did not correlate significantly with the transcript levels of the stress genes studied, but Zn might have triggered transcription of proteins which dealt with subsequent accumulation of Cd. SOD and CAT activities increased in gills after transfer, but MT protein levels decreased. In liver, SOD activity and MT protein levels increased, while in kidney only MT protein concentrations were elevated after transfer. There was a general lack of consistency between mRNA transcription and enzyme activities, indicating that these proteins and enzymes are not solely under transcriptional control. SN - 0045-6535 UR - https://www.unboundmedicine.com/medline/citation/17276485/Induction_and_activity_of_oxidative_stress_related_proteins_during_waterborne_Cd/Zn_exposure_in_brown_trout__Salmo_trutta__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(06)01760-7 DB - PRIME DP - Unbound Medicine ER -