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Activation of endothelial nitric oxide synthase by red wine polyphenols: impact of grape cultivars, growing area and the vinification process.
J Hypertens. 2007 Mar; 25(3):541-9.JH

Abstract

OBJECTIVE

Accumulating data document protective effects of red wine in cardiovascular disease by improved nitric oxide bioavailability. Not all wines, however, seem to be equally effective. The aim of the present study was to elucidate the impact of grape cultivars, growing area and the vinification/fermentation process on the ability of red wine polyphenol extracts to increase human endothelial nitric oxide synthase (eNOS) promotor and enzyme activity.

METHODS

We produced polyphenol extracts from a variety of red wines from all over the world and tested their influence on eNOS promotor activity by a luciferase reporter gene assay. Selected extracts were subjected to the L-arginine-L-citrulline conversion assay to confirm that enhanced eNOS transcription results in enhanced enzyme activity.

RESULTS

We show that polyphenol extracts from wines of specific origin and grape cultivars vary strongly in their individual activity. However, when averaged, the activity could not be attributed to a specific grape cultivar or growing area. Resveratrol alone significantly enhanced eNOS promotor and enzyme activity, although at concentrations higher than those available in effective concentrations of red wine polyphenol extracts. Extracts of grape juice showed activity as well.

CONCLUSIONS

The biological activity of red wines on eNOS transcription depends neither on grape cultivars nor on growing area in general. Resveratrol may be the most promising ingredient in red wine polyphenol extracts, but does not account for the complete effect. A large part of the active components are genuine grape compounds, since extracts of grape juice that did not undergo a fermentation process also showed activity.

Authors+Show Affiliations

Department of Pharmacy, Center of Drug Research, University of Munich, Munich, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17278969

Citation

Räthel, Thomas R., et al. "Activation of Endothelial Nitric Oxide Synthase By Red Wine Polyphenols: Impact of Grape Cultivars, Growing Area and the Vinification Process." Journal of Hypertension, vol. 25, no. 3, 2007, pp. 541-9.
Räthel TR, Samtleben R, Vollmar AM, et al. Activation of endothelial nitric oxide synthase by red wine polyphenols: impact of grape cultivars, growing area and the vinification process. J Hypertens. 2007;25(3):541-9.
Räthel, T. R., Samtleben, R., Vollmar, A. M., & Dirsch, V. M. (2007). Activation of endothelial nitric oxide synthase by red wine polyphenols: impact of grape cultivars, growing area and the vinification process. Journal of Hypertension, 25(3), 541-9.
Räthel TR, et al. Activation of Endothelial Nitric Oxide Synthase By Red Wine Polyphenols: Impact of Grape Cultivars, Growing Area and the Vinification Process. J Hypertens. 2007;25(3):541-9. PubMed PMID: 17278969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of endothelial nitric oxide synthase by red wine polyphenols: impact of grape cultivars, growing area and the vinification process. AU - Räthel,Thomas R, AU - Samtleben,Rainer, AU - Vollmar,Angelika M, AU - Dirsch,Verena M, PY - 2007/2/7/pubmed PY - 2007/3/16/medline PY - 2007/2/7/entrez SP - 541 EP - 9 JF - Journal of hypertension JO - J Hypertens VL - 25 IS - 3 N2 - OBJECTIVE: Accumulating data document protective effects of red wine in cardiovascular disease by improved nitric oxide bioavailability. Not all wines, however, seem to be equally effective. The aim of the present study was to elucidate the impact of grape cultivars, growing area and the vinification/fermentation process on the ability of red wine polyphenol extracts to increase human endothelial nitric oxide synthase (eNOS) promotor and enzyme activity. METHODS: We produced polyphenol extracts from a variety of red wines from all over the world and tested their influence on eNOS promotor activity by a luciferase reporter gene assay. Selected extracts were subjected to the L-arginine-L-citrulline conversion assay to confirm that enhanced eNOS transcription results in enhanced enzyme activity. RESULTS: We show that polyphenol extracts from wines of specific origin and grape cultivars vary strongly in their individual activity. However, when averaged, the activity could not be attributed to a specific grape cultivar or growing area. Resveratrol alone significantly enhanced eNOS promotor and enzyme activity, although at concentrations higher than those available in effective concentrations of red wine polyphenol extracts. Extracts of grape juice showed activity as well. CONCLUSIONS: The biological activity of red wines on eNOS transcription depends neither on grape cultivars nor on growing area in general. Resveratrol may be the most promising ingredient in red wine polyphenol extracts, but does not account for the complete effect. A large part of the active components are genuine grape compounds, since extracts of grape juice that did not undergo a fermentation process also showed activity. SN - 0263-6352 UR - https://www.unboundmedicine.com/medline/citation/17278969/Activation_of_endothelial_nitric_oxide_synthase_by_red_wine_polyphenols:_impact_of_grape_cultivars_growing_area_and_the_vinification_process_ L2 - https://doi.org/10.1097/HJH.0b013e328013e805 DB - PRIME DP - Unbound Medicine ER -