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Effects of maternal undernutrition during lactation on aromatase, estrogen, and androgen receptors expression in rat testis at weaning.
J Endocrinol. 2007 Feb; 192(2):301-11.JE

Abstract

The goal of this study was to evaluate the effects of maternal malnutrition during lactation on serum levels of testosterone and estradiol, testicular testosterone concentration, aromatase, testicular androgen (AR) and estrogen alpha (ERalpha) receptors expression in the pups at weaning. From parturition until weaning, Wistar rats were separated into three groups: (C) control group, with free access to a standard laboratory diet containing 23% protein; protein-energy restricted (PER) group, with free access to an isoenergy and protein-restricted diet containing 8% protein; and energy-restricted (ER) group, receiving standard laboratory diet in restricted quantities, which were calculated according to the mean ingestion of the PER group. All pups were killed at weaning, corresponding to 21 days post partum. Compared with the C group, body weights (C=48 +/- 2.3 g; PER=20 +/- 1.3 g; ER=25.4 +/- 0.9 g; P<0.01) and testicular weights (C=0.15 +/- 0.02 g, PER=0.05 +/- 0.01 g, ER=0.06 +/- 0.02 g, P < 0.001) of both PER and ER groups were lower. However, there was no significant difference in the testicular/body weight ratio in PER and ER groups compared with the C group. The testosterone serum concentration (ng/ml) was significantly higher in the PER group compared with ER and C groups (C=0.09 +/- 0.012; PER=0.45 +/- 0.04; ER=0.15 +/- 0.03, P < 0.01). Testicular testosterone concentration (C=2.1 +/- 0.43; PER=6.5 +/- 0.7; ER=13 +/- 2.3, P < 0.01) was increased in treated groups when compared with controls. The estradiol serum concentration (pg/ml) was lower in both dietary groups (C=74 +/- 4.6; PER=49 +/- 3.2; ER=60 +/- 5.5, P < 0.01). The amounts of aromatase mRNA and ERalpha transcripts were significantly lower (P<0.05) in PER and ER groups; conversely AR (both mRNA and protein) was significantly enhanced (P<0.05) in treated animals. The nutritional state in early phases of development is important since we have demonstrated here that the maternal malnutrition during lactation leads to alterations in estradiol and testosterone serum concentrations, testicular testosterone concentration, AR and ERalpha expression together with a decrease of aromatase expression. All together, these changes of steroid status may be deleterious for future germ cell development and reproductive function of these male pups submitted to early malnutrition.

Authors+Show Affiliations

Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17283230

Citation

Teixeira, Cíntia Vilanova, et al. "Effects of Maternal Undernutrition During Lactation On Aromatase, Estrogen, and Androgen Receptors Expression in Rat Testis at Weaning." The Journal of Endocrinology, vol. 192, no. 2, 2007, pp. 301-11.
Teixeira CV, Silandre D, de Souza Santos AM, et al. Effects of maternal undernutrition during lactation on aromatase, estrogen, and androgen receptors expression in rat testis at weaning. J Endocrinol. 2007;192(2):301-11.
Teixeira, C. V., Silandre, D., de Souza Santos, A. M., Delalande, C., Sampaio, F. J., Carreau, S., & da Fonte Ramos, C. (2007). Effects of maternal undernutrition during lactation on aromatase, estrogen, and androgen receptors expression in rat testis at weaning. The Journal of Endocrinology, 192(2), 301-11.
Teixeira CV, et al. Effects of Maternal Undernutrition During Lactation On Aromatase, Estrogen, and Androgen Receptors Expression in Rat Testis at Weaning. J Endocrinol. 2007;192(2):301-11. PubMed PMID: 17283230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of maternal undernutrition during lactation on aromatase, estrogen, and androgen receptors expression in rat testis at weaning. AU - Teixeira,Cíntia Vilanova, AU - Silandre,Dorothée, AU - de Souza Santos,Alba Marcelly, AU - Delalande,Christelle, AU - Sampaio,Francisco J B, AU - Carreau,Serge, AU - da Fonte Ramos,Cristiane, PY - 2007/2/7/pubmed PY - 2007/4/14/medline PY - 2007/2/7/entrez SP - 301 EP - 11 JF - The Journal of endocrinology JO - J Endocrinol VL - 192 IS - 2 N2 - The goal of this study was to evaluate the effects of maternal malnutrition during lactation on serum levels of testosterone and estradiol, testicular testosterone concentration, aromatase, testicular androgen (AR) and estrogen alpha (ERalpha) receptors expression in the pups at weaning. From parturition until weaning, Wistar rats were separated into three groups: (C) control group, with free access to a standard laboratory diet containing 23% protein; protein-energy restricted (PER) group, with free access to an isoenergy and protein-restricted diet containing 8% protein; and energy-restricted (ER) group, receiving standard laboratory diet in restricted quantities, which were calculated according to the mean ingestion of the PER group. All pups were killed at weaning, corresponding to 21 days post partum. Compared with the C group, body weights (C=48 +/- 2.3 g; PER=20 +/- 1.3 g; ER=25.4 +/- 0.9 g; P<0.01) and testicular weights (C=0.15 +/- 0.02 g, PER=0.05 +/- 0.01 g, ER=0.06 +/- 0.02 g, P < 0.001) of both PER and ER groups were lower. However, there was no significant difference in the testicular/body weight ratio in PER and ER groups compared with the C group. The testosterone serum concentration (ng/ml) was significantly higher in the PER group compared with ER and C groups (C=0.09 +/- 0.012; PER=0.45 +/- 0.04; ER=0.15 +/- 0.03, P < 0.01). Testicular testosterone concentration (C=2.1 +/- 0.43; PER=6.5 +/- 0.7; ER=13 +/- 2.3, P < 0.01) was increased in treated groups when compared with controls. The estradiol serum concentration (pg/ml) was lower in both dietary groups (C=74 +/- 4.6; PER=49 +/- 3.2; ER=60 +/- 5.5, P < 0.01). The amounts of aromatase mRNA and ERalpha transcripts were significantly lower (P<0.05) in PER and ER groups; conversely AR (both mRNA and protein) was significantly enhanced (P<0.05) in treated animals. The nutritional state in early phases of development is important since we have demonstrated here that the maternal malnutrition during lactation leads to alterations in estradiol and testosterone serum concentrations, testicular testosterone concentration, AR and ERalpha expression together with a decrease of aromatase expression. All together, these changes of steroid status may be deleterious for future germ cell development and reproductive function of these male pups submitted to early malnutrition. SN - 0022-0795 UR - https://www.unboundmedicine.com/medline/citation/17283230/Effects_of_maternal_undernutrition_during_lactation_on_aromatase_estrogen_and_androgen_receptors_expression_in_rat_testis_at_weaning_ L2 - https://joe.bioscientifica.com/doi/10.1677/joe.1.06712 DB - PRIME DP - Unbound Medicine ER -