Tags

Type your tag names separated by a space and hit enter

In vivo administration of epidermal growth factor and its homologue attenuates developmental maturation of functional excitatory synapses in cortical GABAergic neurons.
Eur J Neurosci 2007; 25(2):380-90EJ

Abstract

The ErbB1 ligand family includes epidermal growth factor (EGF), transforming growth factor-alpha (TGFalpha), heparin-binding EGF-like growth factor, amphiregulin and betacellulin. Previously, we demonstrated that TGFalpha decreases alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors in cultured neocortical gamma-aminobutyric acid (GABA) neurons. In the present study, we examined in vivo effects of EGF and TGFalpha in the mouse neocortex using electrophysiological and biochemical techniques. In mouse neonates, subcutaneously administered EGF penetrated the blood-brain barrier and activated ErbB1 in the neocortex. Daily administration of EGF or TGFalpha attenuates developmental increases in expression of the AMPA receptor subunits (GluR1 and GluR2/3) in the neocortex of postnatal mice. Immunohistochemistry revealed that the reduction in AMPA receptor expression was significant in the GABAergic neurons, especially those positive for parvalbumin. Using cortical slices prepared from EGF-treated mice, we recorded miniature excitatory postsynaptic currents (mEPSCs) in both GABAergic and pyramidal neurons. Subchronic treatment with EGF decreased the amplitude and frequency of mEPSCs in GABAergic neurons, but its effects were negligible on pyramidal neurons. We conclude that EGF or other ErbB1 ligand(s) attenuates a developmental increase in AMPA receptor expression and function in cortical GABAergic neurons.

Authors+Show Affiliations

Molecular Neurobiology, Brain Research Institute, Niigata University, 951-8585 Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17284178

Citation

Nagano, Tadasato, et al. "In Vivo Administration of Epidermal Growth Factor and Its Homologue Attenuates Developmental Maturation of Functional Excitatory Synapses in Cortical GABAergic Neurons." The European Journal of Neuroscience, vol. 25, no. 2, 2007, pp. 380-90.
Nagano T, Namba H, Abe Y, et al. In vivo administration of epidermal growth factor and its homologue attenuates developmental maturation of functional excitatory synapses in cortical GABAergic neurons. Eur J Neurosci. 2007;25(2):380-90.
Nagano, T., Namba, H., Abe, Y., Aoki, H., Takei, N., & Nawa, H. (2007). In vivo administration of epidermal growth factor and its homologue attenuates developmental maturation of functional excitatory synapses in cortical GABAergic neurons. The European Journal of Neuroscience, 25(2), pp. 380-90.
Nagano T, et al. In Vivo Administration of Epidermal Growth Factor and Its Homologue Attenuates Developmental Maturation of Functional Excitatory Synapses in Cortical GABAergic Neurons. Eur J Neurosci. 2007;25(2):380-90. PubMed PMID: 17284178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo administration of epidermal growth factor and its homologue attenuates developmental maturation of functional excitatory synapses in cortical GABAergic neurons. AU - Nagano,Tadasato, AU - Namba,Hisaaki, AU - Abe,Yuichi, AU - Aoki,Hiroyuki, AU - Takei,Nobuyuki, AU - Nawa,Hiroyuki, PY - 2007/2/8/pubmed PY - 2007/4/6/medline PY - 2007/2/8/entrez SP - 380 EP - 90 JF - The European journal of neuroscience JO - Eur. J. Neurosci. VL - 25 IS - 2 N2 - The ErbB1 ligand family includes epidermal growth factor (EGF), transforming growth factor-alpha (TGFalpha), heparin-binding EGF-like growth factor, amphiregulin and betacellulin. Previously, we demonstrated that TGFalpha decreases alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors in cultured neocortical gamma-aminobutyric acid (GABA) neurons. In the present study, we examined in vivo effects of EGF and TGFalpha in the mouse neocortex using electrophysiological and biochemical techniques. In mouse neonates, subcutaneously administered EGF penetrated the blood-brain barrier and activated ErbB1 in the neocortex. Daily administration of EGF or TGFalpha attenuates developmental increases in expression of the AMPA receptor subunits (GluR1 and GluR2/3) in the neocortex of postnatal mice. Immunohistochemistry revealed that the reduction in AMPA receptor expression was significant in the GABAergic neurons, especially those positive for parvalbumin. Using cortical slices prepared from EGF-treated mice, we recorded miniature excitatory postsynaptic currents (mEPSCs) in both GABAergic and pyramidal neurons. Subchronic treatment with EGF decreased the amplitude and frequency of mEPSCs in GABAergic neurons, but its effects were negligible on pyramidal neurons. We conclude that EGF or other ErbB1 ligand(s) attenuates a developmental increase in AMPA receptor expression and function in cortical GABAergic neurons. SN - 0953-816X UR - https://www.unboundmedicine.com/medline/citation/17284178/In_vivo_administration_of_epidermal_growth_factor_and_its_homologue_attenuates_developmental_maturation_of_functional_excitatory_synapses_in_cortical_GABAergic_neurons_ L2 - https://doi.org/10.1111/j.1460-9568.2007.05297.x DB - PRIME DP - Unbound Medicine ER -