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[Inhibiton of experimental autoimmune encephalomyelitis in Lewis rats by nasal administration of encephalitogenic MBP peptides: synergistic effects of MBP 68-86 and 87-99].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Feb; 23(2):106-9.XB

Abstract

AIM

To explore the synergistic effect of MBP 68-86 and 87-99, on the inhibition of experimental autoimmune encephalomyelitis (EAE) in Lewis rat by nasal administration.

METHODS

Three different MBP peptides(MBP 68-86, 87-99, and the non-encephalitogenic peptide 110-128) were synthesized and administrated nasally to Lewis rat on day-11, -10, -9, -8 and -7 prior to immunization with the guinea pig MBP (gp-MBP)+CFA, which was used to induce EAE. The protective effect on Lewis rat from EAE by the MBP peptides was evaluated.

RESULTS

Protection was achieved with the encephalitogenic peptides MBP 68-86 and 87-99, MBP 68-86 being more potent, but not with MBP 110-128. Neither MBP 68-86 nor 87-99 used alone conferred complete protection to gp-MBP-induced EAE. In contrast, nasal administration of a mixture of MBP 68-86 and 87-99 completely blocked gp-MBP-induced EAE even at lower dosage than being used alone. Rats tolerized with MBP 68-86+87-99 nasally showed decreased T cell responses to MBP, reflected by lymphocyte proliferation and IFN-gamma ELISPOT assays. Rats tolerized with MBP 68-86+87-99 also had abrogated MBP-reactive IFN-gamma and TNF-alpha mRNA expression in lymph node cells compared to rats receiving MBP 110-128 nasally, while similar low levels of MBP-reactive TGF-beta and IL-4 mRNA expressing cells were observed in the two groups.

CONCLUSION

Nasal administration of encephalitogenic MBP peptides can induce antigen-specific T cell tolerance and confer incomplete protection to gp-MBP-induced EAE, and MBP 68-86 and 87-99 have synergistic effects. Non-regulatory mechanisms are proposed to be responsible for tolerance development after nasal peptide administration.

Authors+Show Affiliations

Department of Neurobiology, Harbin Medical University, Harbin 150086, China. sunbo720@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17286899

Citation

Sun, Bo, et al. "[Inhibiton of Experimental Autoimmune Encephalomyelitis in Lewis Rats By Nasal Administration of Encephalitogenic MBP Peptides: Synergistic Effects of MBP 68-86 and 87-99]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 23, no. 2, 2007, pp. 106-9.
Sun B, Yang S, Peng HS, et al. [Inhibiton of experimental autoimmune encephalomyelitis in Lewis rats by nasal administration of encephalitogenic MBP peptides: synergistic effects of MBP 68-86 and 87-99]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007;23(2):106-9.
Sun, B., Yang, S., Peng, H. S., Qiao, H., Cao, J. Y., Jin, L. H., & Li, H. L. (2007). [Inhibiton of experimental autoimmune encephalomyelitis in Lewis rats by nasal administration of encephalitogenic MBP peptides: synergistic effects of MBP 68-86 and 87-99]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 23(2), 106-9.
Sun B, et al. [Inhibiton of Experimental Autoimmune Encephalomyelitis in Lewis Rats By Nasal Administration of Encephalitogenic MBP Peptides: Synergistic Effects of MBP 68-86 and 87-99]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007;23(2):106-9. PubMed PMID: 17286899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Inhibiton of experimental autoimmune encephalomyelitis in Lewis rats by nasal administration of encephalitogenic MBP peptides: synergistic effects of MBP 68-86 and 87-99]. AU - Sun,Bo, AU - Yang,Shuo, AU - Peng,Hai-sheng, AU - Qiao,Hui, AU - Cao,Jing-yan, AU - Jin,Lian-hong, AU - Li,Hu-lun, PY - 2007/2/9/pubmed PY - 2009/5/1/medline PY - 2007/2/9/entrez SP - 106 EP - 9 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 23 IS - 2 N2 - AIM: To explore the synergistic effect of MBP 68-86 and 87-99, on the inhibition of experimental autoimmune encephalomyelitis (EAE) in Lewis rat by nasal administration. METHODS: Three different MBP peptides(MBP 68-86, 87-99, and the non-encephalitogenic peptide 110-128) were synthesized and administrated nasally to Lewis rat on day-11, -10, -9, -8 and -7 prior to immunization with the guinea pig MBP (gp-MBP)+CFA, which was used to induce EAE. The protective effect on Lewis rat from EAE by the MBP peptides was evaluated. RESULTS: Protection was achieved with the encephalitogenic peptides MBP 68-86 and 87-99, MBP 68-86 being more potent, but not with MBP 110-128. Neither MBP 68-86 nor 87-99 used alone conferred complete protection to gp-MBP-induced EAE. In contrast, nasal administration of a mixture of MBP 68-86 and 87-99 completely blocked gp-MBP-induced EAE even at lower dosage than being used alone. Rats tolerized with MBP 68-86+87-99 nasally showed decreased T cell responses to MBP, reflected by lymphocyte proliferation and IFN-gamma ELISPOT assays. Rats tolerized with MBP 68-86+87-99 also had abrogated MBP-reactive IFN-gamma and TNF-alpha mRNA expression in lymph node cells compared to rats receiving MBP 110-128 nasally, while similar low levels of MBP-reactive TGF-beta and IL-4 mRNA expressing cells were observed in the two groups. CONCLUSION: Nasal administration of encephalitogenic MBP peptides can induce antigen-specific T cell tolerance and confer incomplete protection to gp-MBP-induced EAE, and MBP 68-86 and 87-99 have synergistic effects. Non-regulatory mechanisms are proposed to be responsible for tolerance development after nasal peptide administration. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/17286899/[Inhibiton_of_experimental_autoimmune_encephalomyelitis_in_Lewis_rats_by_nasal_administration_of_encephalitogenic_MBP_peptides:_synergistic_effects_of_MBP_68_86_and_87_99]_ DB - PRIME DP - Unbound Medicine ER -