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[Determination of nicotine and cotinine in human biological materials and their significance in toxicological studies].
Przegl Lek. 2006; 63(10):892-6.PL

Abstract

The aim of this study was the preparation of reliable procedure of the determination of nicotine and cotinine both in classic (serum, urine) and alternative biological materials (hair, saliva) and evaluation of their significance for clinical and forensic toxicology. Biological material samples (blood, urine, saliva) were taken from patients after Percutaneous Trans-luminal Coronary Angioplasty (PTCA). The determination of cotinine and nicotine concentration in the biological material should be optimized depending on the aim of analysis. Liquid-liquid extraction procedure and high performance liquid chromatography HPLC/UV-DAD are reliable, specific and relatively cheap. Serum and saliva are valuable biological materials which allow to determine temporary nicotine and cotinine content on the similar level of concentrations. In the near future it will be able to replace blood with saliva sample because of an easy and non-invasive way of sampling. Evaluation of cotinine concentration in urine allows to distinguish the passive from the active tobacco smokers. Hair analysis allows to control a nicotine abstinence as well as a long-term evaluation of the history of smoking. However usage of hair is limited because of difficulty with sampling. Interpretation of results in analysis of alternative materials (hair, saliva) pose a problem because of lack of sampling standardization and lack of standardization of final analysis method.

Authors+Show Affiliations

Wiergowski M
Katedra i Zakład Medycyny Sadowej, Akademii Medycznej w Gdańsku. marwier@amg.gda.pl
Nowak-Banasik L
No affiliation info available
Morkowska A
No affiliation info available
Galer-Tatarowicz K
No affiliation info available
Szpiech B
No affiliation info available
Korolkiewicz R
No affiliation info available
Anand JS
No affiliation info available

MeSH

AgedChromatography, High Pressure LiquidClinical Chemistry TestsCotinineDiagnosis, DifferentialFemaleForensic ToxicologyHairHumansInhalation ExposureMaleMiddle AgedNicotineSalivaSensitivity and SpecificitySmokingSpecimen HandlingTobacco Smoke PollutionTobacco Use Disorder

Pub Type(s)

English Abstract
Journal Article

Language

pol

PubMed ID

17288178

Citation

Wiergowski, Marek, et al. "[Determination of Nicotine and Cotinine in Human Biological Materials and Their Significance in Toxicological Studies]." Przeglad Lekarski, vol. 63, no. 10, 2006, pp. 892-6.
Wiergowski M, Nowak-Banasik L, Morkowska A, et al. [Determination of nicotine and cotinine in human biological materials and their significance in toxicological studies]. Przegl Lek. 2006;63(10):892-6.
Wiergowski, M., Nowak-Banasik, L., Morkowska, A., Galer-Tatarowicz, K., Szpiech, B., Korolkiewicz, R., & Anand, J. S. (2006). [Determination of nicotine and cotinine in human biological materials and their significance in toxicological studies]. Przeglad Lekarski, 63(10), 892-6.
Wiergowski M, et al. [Determination of Nicotine and Cotinine in Human Biological Materials and Their Significance in Toxicological Studies]. Przegl Lek. 2006;63(10):892-6. PubMed PMID: 17288178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of nicotine and cotinine in human biological materials and their significance in toxicological studies]. AU - Wiergowski,Marek, AU - Nowak-Banasik,Livia, AU - Morkowska,Anna, AU - Galer-Tatarowicz,Katarzyna, AU - Szpiech,Beata, AU - Korolkiewicz,Roman, AU - Anand,Jacek Sein, PY - 2007/2/10/pubmed PY - 2007/3/16/medline PY - 2007/2/10/entrez SP - 892 EP - 6 JF - Przeglad lekarski JO - Przegl Lek VL - 63 IS - 10 N2 - The aim of this study was the preparation of reliable procedure of the determination of nicotine and cotinine both in classic (serum, urine) and alternative biological materials (hair, saliva) and evaluation of their significance for clinical and forensic toxicology. Biological material samples (blood, urine, saliva) were taken from patients after Percutaneous Trans-luminal Coronary Angioplasty (PTCA). The determination of cotinine and nicotine concentration in the biological material should be optimized depending on the aim of analysis. Liquid-liquid extraction procedure and high performance liquid chromatography HPLC/UV-DAD are reliable, specific and relatively cheap. Serum and saliva are valuable biological materials which allow to determine temporary nicotine and cotinine content on the similar level of concentrations. In the near future it will be able to replace blood with saliva sample because of an easy and non-invasive way of sampling. Evaluation of cotinine concentration in urine allows to distinguish the passive from the active tobacco smokers. Hair analysis allows to control a nicotine abstinence as well as a long-term evaluation of the history of smoking. However usage of hair is limited because of difficulty with sampling. Interpretation of results in analysis of alternative materials (hair, saliva) pose a problem because of lack of sampling standardization and lack of standardization of final analysis method. SN - 0033-2240 UR - https://www.unboundmedicine.com/medline/citation/17288178/[Determination_of_nicotine_and_cotinine_in_human_biological_materials_and_their_significance_in_toxicological_studies]_ DB - PRIME DP - Unbound Medicine ER -