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Spatial distributions of Kv4 channels and KChip2 isoforms in the murine heart based on laser capture microdissection.
Cardiovasc Res. 2007 Mar 01; 73(4):739-49.CR

Abstract

OBJECTIVE

Regional differences in repolarizing K(+) current densities and expression levels of their molecular components are important for coordinating the pattern of electrical excitation and repolarization of the heart. The small size of hearts from mice may obscure these interventricular and/or transmural expression differences of K(+) channels. We have examined this possibility in adult mouse ventricle using a technology that provides very high spatial resolution of tissue collection.

METHODS

Conventional manual dissection and laser capture microdissection (LCM) were utilized to dissect tissue from distinct ventricular regions. RNA was isolated from epicardial, mid-myocardial and endocardial layers of both the right and left ventricles. Real-time RT-PCR was used to quantify the transcript expression in these different regions.

RESULTS

LCM revealed significant interventricular and transmural gradients for both Kv4.2 and the alpha-subunit of KChIP2. The expression profile of a second K(+) channel transcript, Kir2.1, which is responsible for the inwardly rectifying K(+) current I(k1), showed no interventricular or transmural gradients and therefore served as a negative control.

CONCLUSIONS

Our findings are in contrast to previous reports of a relatively uniform left ventricular transmural pattern of expression of Kv4.2, Kv4.3 and KChIP2 in adult mouse heart, which appear to be different than that in larger mammals. Specifically, our results demonstrate significant epi- to endocardial differences in the patterns of expression of both Kv4.2 and KChIP2.

Authors+Show Affiliations

Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17289005

Citation

Teutsch, Christine, et al. "Spatial Distributions of Kv4 Channels and KChip2 Isoforms in the Murine Heart Based On Laser Capture Microdissection." Cardiovascular Research, vol. 73, no. 4, 2007, pp. 739-49.
Teutsch C, Kondo RP, Dederko DA, et al. Spatial distributions of Kv4 channels and KChip2 isoforms in the murine heart based on laser capture microdissection. Cardiovasc Res. 2007;73(4):739-49.
Teutsch, C., Kondo, R. P., Dederko, D. A., Chrast, J., Chien, K. R., & Giles, W. R. (2007). Spatial distributions of Kv4 channels and KChip2 isoforms in the murine heart based on laser capture microdissection. Cardiovascular Research, 73(4), 739-49.
Teutsch C, et al. Spatial Distributions of Kv4 Channels and KChip2 Isoforms in the Murine Heart Based On Laser Capture Microdissection. Cardiovasc Res. 2007 Mar 1;73(4):739-49. PubMed PMID: 17289005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spatial distributions of Kv4 channels and KChip2 isoforms in the murine heart based on laser capture microdissection. AU - Teutsch,Christine, AU - Kondo,Richard P, AU - Dederko,Dorothy A, AU - Chrast,Jacqueline, AU - Chien,Kenneth R, AU - Giles,Wayne R, Y1 - 2006/11/30/ PY - 2006/05/28/received PY - 2006/11/06/revised PY - 2006/11/27/accepted PY - 2007/2/10/pubmed PY - 2007/5/22/medline PY - 2007/2/10/entrez SP - 739 EP - 49 JF - Cardiovascular research JO - Cardiovasc Res VL - 73 IS - 4 N2 - OBJECTIVE: Regional differences in repolarizing K(+) current densities and expression levels of their molecular components are important for coordinating the pattern of electrical excitation and repolarization of the heart. The small size of hearts from mice may obscure these interventricular and/or transmural expression differences of K(+) channels. We have examined this possibility in adult mouse ventricle using a technology that provides very high spatial resolution of tissue collection. METHODS: Conventional manual dissection and laser capture microdissection (LCM) were utilized to dissect tissue from distinct ventricular regions. RNA was isolated from epicardial, mid-myocardial and endocardial layers of both the right and left ventricles. Real-time RT-PCR was used to quantify the transcript expression in these different regions. RESULTS: LCM revealed significant interventricular and transmural gradients for both Kv4.2 and the alpha-subunit of KChIP2. The expression profile of a second K(+) channel transcript, Kir2.1, which is responsible for the inwardly rectifying K(+) current I(k1), showed no interventricular or transmural gradients and therefore served as a negative control. CONCLUSIONS: Our findings are in contrast to previous reports of a relatively uniform left ventricular transmural pattern of expression of Kv4.2, Kv4.3 and KChIP2 in adult mouse heart, which appear to be different than that in larger mammals. Specifically, our results demonstrate significant epi- to endocardial differences in the patterns of expression of both Kv4.2 and KChIP2. SN - 0008-6363 UR - https://www.unboundmedicine.com/medline/citation/17289005/Spatial_distributions_of_Kv4_channels_and_KChip2_isoforms_in_the_murine_heart_based_on_laser_capture_microdissection_ L2 - https://academic.oup.com/cardiovascres/article-lookup/doi/10.1016/j.cardiores.2006.11.034 DB - PRIME DP - Unbound Medicine ER -