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[Effect of vascular endothelial growth factor on pre-eclampsia in pregnant rats].
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2007 01; 36(1):84-7.ZD

Abstract

OBJECTIVE

To investigate relationship between the vascular endothelial growth factor (VEGF) and the pathogenesis of pre-eclampsia in pregnant rats.

METHODS

Pregnant rats were divided into two groups randomly. Saline solution or L-nitro arginine methyl ester (L-NAME) 125 mg/d was given subcutaneously from day 7 of gestation till establishing pre-eclampsia. Systolic blood pressure, urine protein, platelet count, and weight of pups and placenta were determined. The levels of VEGF in pregnant rats venous serum, placenta and decidual tissue from normal pregnancy and pre-eclampsia rats were detected by ELISA and immunohistochemistry, respectively.

RESULT

Pregnant rats which were given L-NAME produced physical signs similar to those of pre-eclampsia, such as increase in systolic blood pressure [(145.3 +/-4.6)mmHg] and urine protein [(814.3 +/-57.5)mg/L], and decrease in platelet count [(467.1 +/-76.3) x 10(9)/L] and weight of pups and placenta. Compared with controls, the intensity of VEGF immunostaining in trophoblast or decidual cells were significantly reduced. The serum levels of VEGF were significantly lower in pre-eclampsia group than in normal pregnancy.

CONCLUSION

Decreased serum levels of VEGF and reduced expression of VEGF in placental tissues might in part explain the pathogenesis of pre-eclampsia in pregnant rats.

Authors+Show Affiliations

Chen DQ
The Affiliated Obstetrics and Gynecology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China. chendq@zju.edu.cn
Lu XJ
No affiliation info available
Dong MY
No affiliation info available
Wang ZP
No affiliation info available
He J
No affiliation info available

MeSH

AnimalsDeciduaEnzyme-Linked Immunosorbent AssayFemaleImmunohistochemistryNG-Nitroarginine Methyl EsterPlacentaPre-EclampsiaPregnancyRandom AllocationRatsRats, WistarVascular Endothelial Growth Factor A

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17290497

Citation

Chen, Dan-Qing, et al. "[Effect of Vascular Endothelial Growth Factor On Pre-eclampsia in Pregnant Rats]." Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences, vol. 36, no. 1, 2007, pp. 84-7.
Chen DQ, Lu XJ, Dong MY, et al. [Effect of vascular endothelial growth factor on pre-eclampsia in pregnant rats]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2007;36(1):84-7.
Chen, D. Q., Lu, X. J., Dong, M. Y., Wang, Z. P., & He, J. (2007). [Effect of vascular endothelial growth factor on pre-eclampsia in pregnant rats]. Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences, 36(1), 84-7.
Chen DQ, et al. [Effect of Vascular Endothelial Growth Factor On Pre-eclampsia in Pregnant Rats]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2007;36(1):84-7. PubMed PMID: 17290497.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of vascular endothelial growth factor on pre-eclampsia in pregnant rats]. AU - Chen,Dan-Qing, AU - Lu,Xiao-Juan, AU - Dong,Min-Yue, AU - Wang,Zheng-Ping, AU - He,Jing, PY - 2007/2/10/pubmed PY - 2008/5/6/medline PY - 2007/2/10/entrez SP - 84 EP - 7 JF - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences JO - Zhejiang Da Xue Xue Bao Yi Xue Ban VL - 36 IS - 1 N2 - OBJECTIVE: To investigate relationship between the vascular endothelial growth factor (VEGF) and the pathogenesis of pre-eclampsia in pregnant rats. METHODS: Pregnant rats were divided into two groups randomly. Saline solution or L-nitro arginine methyl ester (L-NAME) 125 mg/d was given subcutaneously from day 7 of gestation till establishing pre-eclampsia. Systolic blood pressure, urine protein, platelet count, and weight of pups and placenta were determined. The levels of VEGF in pregnant rats venous serum, placenta and decidual tissue from normal pregnancy and pre-eclampsia rats were detected by ELISA and immunohistochemistry, respectively. RESULT: Pregnant rats which were given L-NAME produced physical signs similar to those of pre-eclampsia, such as increase in systolic blood pressure [(145.3 +/-4.6)mmHg] and urine protein [(814.3 +/-57.5)mg/L], and decrease in platelet count [(467.1 +/-76.3) x 10(9)/L] and weight of pups and placenta. Compared with controls, the intensity of VEGF immunostaining in trophoblast or decidual cells were significantly reduced. The serum levels of VEGF were significantly lower in pre-eclampsia group than in normal pregnancy. CONCLUSION: Decreased serum levels of VEGF and reduced expression of VEGF in placental tissues might in part explain the pathogenesis of pre-eclampsia in pregnant rats. SN - 1008-9292 UR - https://www.unboundmedicine.com/medline/citation/17290497/[Effect_of_vascular_endothelial_growth_factor_on_pre_eclampsia_in_pregnant_rats]_ DB - PRIME DP - Unbound Medicine ER -