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Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA.

Abstract

Inactivated vaccines of Avibacterium paragallinarum provide protection and reduce the economic losses caused by infectious coryza. However, inactivated bacterins provide protection only against the Page serovars included in the vaccine. In this study, we investigated the immunological properties of a functional recombinant haemagglutinin protein (rHagA) derived from a Taiwan isolate strain A9 as the immunogen for vaccination. The rHagA subunit vaccine protected 71% of immunized chickens against 10(10) colony-forming units (CFU) of viable A9. Vaccinated chickens which showed no clinical signs of coryza developed haemagglutination inhibition (HI) titers of 1:10 or greater. Haemagglutination (HA) of serovars A and C was not affected by the presence of rHagA specific antiserum. The HA of rHagA could only be induced against formaldehyde-fixed chicken red blood cells (FA-RBCs). These results suggested that HagA is a moderate immunogen and might not be a major haemagglutinin in vivo. However, HagA might be involved in haemagglutination when treated serovar C aggregates fixed RBCs in vitro.

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  • Authors+Show Affiliations

    ,

    Department of Biological Science and Technology, China Medical University, Taichung, Taiwan. yuanmh@mail.cmu.edu.tw

    , , ,

    Source

    Veterinary microbiology 122:3-4 2007 Jun 21 pg 280-9

    MeSH

    Animals
    Antibodies, Bacterial
    Bacterial Vaccines
    Chickens
    Colony Count, Microbial
    Haemophilus Infections
    Haemophilus paragallinarum
    Hemagglutination Inhibition Tests
    Hemagglutinins
    Poultry Diseases
    Recombinant Proteins
    Specific Pathogen-Free Organisms
    Vaccines, Inactivated

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17292567

    Citation

    Hsu, Yuan-Man, et al. "Immunogenicity and Haemagglutination of Recombinant Avibacterium Paragallinarum HagA." Veterinary Microbiology, vol. 122, no. 3-4, 2007, pp. 280-9.
    Hsu YM, Shieh HK, Chen WH, et al. Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA. Vet Microbiol. 2007;122(3-4):280-9.
    Hsu, Y. M., Shieh, H. K., Chen, W. H., Shiang, J. H., & Chang, P. C. (2007). Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA. Veterinary Microbiology, 122(3-4), pp. 280-9.
    Hsu YM, et al. Immunogenicity and Haemagglutination of Recombinant Avibacterium Paragallinarum HagA. Vet Microbiol. 2007 Jun 21;122(3-4):280-9. PubMed PMID: 17292567.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA. AU - Hsu,Yuan-Man, AU - Shieh,Happy K, AU - Chen,Wei-Hao, AU - Shiang,Jia-Hsiang, AU - Chang,Poa-Chun, Y1 - 2007/01/23/ PY - 2006/06/07/received PY - 2007/01/15/revised PY - 2007/01/17/accepted PY - 2007/2/13/pubmed PY - 2007/8/1/medline PY - 2007/2/13/entrez SP - 280 EP - 9 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 122 IS - 3-4 N2 - Inactivated vaccines of Avibacterium paragallinarum provide protection and reduce the economic losses caused by infectious coryza. However, inactivated bacterins provide protection only against the Page serovars included in the vaccine. In this study, we investigated the immunological properties of a functional recombinant haemagglutinin protein (rHagA) derived from a Taiwan isolate strain A9 as the immunogen for vaccination. The rHagA subunit vaccine protected 71% of immunized chickens against 10(10) colony-forming units (CFU) of viable A9. Vaccinated chickens which showed no clinical signs of coryza developed haemagglutination inhibition (HI) titers of 1:10 or greater. Haemagglutination (HA) of serovars A and C was not affected by the presence of rHagA specific antiserum. The HA of rHagA could only be induced against formaldehyde-fixed chicken red blood cells (FA-RBCs). These results suggested that HagA is a moderate immunogen and might not be a major haemagglutinin in vivo. However, HagA might be involved in haemagglutination when treated serovar C aggregates fixed RBCs in vitro. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/17292567/Immunogenicity_and_haemagglutination_of_recombinant_Avibacterium_paragallinarum_HagA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(07)00039-9 DB - PRIME DP - Unbound Medicine ER -