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Inactivated influenza H5N1 whole-virus vaccine with aluminum adjuvant induces homologous and heterologous protective immunities against lethal challenge with highly pathogenic H5N1 avian influenza viruses in a mouse model.
Vaccine. 2007 May 04; 25(18):3554-60.V

Abstract

In response to recent outbreaks of H5N1 highly pathogenic avian influenza virus (HPAIV), the development of an effective H5N1 influenza vaccine is urgently important. We assessed the efficacy of two inactivated H5N1 whole-virus vaccines, rgHK213/03 and rgVNJP1203/04, generated by reverse genetics in a mouse model in the presence or absence of aluminum hydroxide (alum) adjuvant. Mice immunized with rgHK213/03 vaccine produced sufficient levels of serum antibodies that were cross-reactive to recent heterologous HPAIV-H5N1 virus, A/Turkey/12/06. The vaccinated mice also elicited protective immunity against challenge with both homologous and heterologous HPAIV-H5N1 viruses. These immune responses were enhanced by addition of alum adjuvant, resulting in antigen sparing of vaccine. On the other hand, mice immunized with rgVNJP1203/04 vaccine had low levels of serum antibodies and less protective immunity than that elicited with rgHK213/03 vaccine regardless of addition of alum adjuvant. Our study suggests that rgHK213/03 vaccine is still useful as a backup vaccine for recent H5N1 viruses and that if rgVNJP1203/04 vaccine is employed, more vaccine antigen would be necessary to induce sufficient immunity.

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Authors+Show Affiliations

Ninomiya A
Laboratory of Influenza Viruses, Department of Virology 3, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-Murayama, Tokyo 208-0011, Japan.
Imai M
No affiliation info available
Tashiro M
No affiliation info available
Odagiri T
No affiliation info available

MeSH

Adjuvants, ImmunologicAluminum HydroxideAnimalsAntibodies, ViralCross ReactionsDisease Models, AnimalHumansImmunizationInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesMiceOrthomyxoviridae InfectionsRecombination, GeneticVaccines, Inactivated

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17293015

Citation

Ninomiya, Ai, et al. "Inactivated Influenza H5N1 Whole-virus Vaccine With Aluminum Adjuvant Induces Homologous and Heterologous Protective Immunities Against Lethal Challenge With Highly Pathogenic H5N1 Avian Influenza Viruses in a Mouse Model." Vaccine, vol. 25, no. 18, 2007, pp. 3554-60.
Ninomiya A, Imai M, Tashiro M, et al. Inactivated influenza H5N1 whole-virus vaccine with aluminum adjuvant induces homologous and heterologous protective immunities against lethal challenge with highly pathogenic H5N1 avian influenza viruses in a mouse model. Vaccine. 2007;25(18):3554-60.
Ninomiya, A., Imai, M., Tashiro, M., & Odagiri, T. (2007). Inactivated influenza H5N1 whole-virus vaccine with aluminum adjuvant induces homologous and heterologous protective immunities against lethal challenge with highly pathogenic H5N1 avian influenza viruses in a mouse model. Vaccine, 25(18), 3554-60.
Ninomiya A, et al. Inactivated Influenza H5N1 Whole-virus Vaccine With Aluminum Adjuvant Induces Homologous and Heterologous Protective Immunities Against Lethal Challenge With Highly Pathogenic H5N1 Avian Influenza Viruses in a Mouse Model. Vaccine. 2007 May 4;25(18):3554-60. PubMed PMID: 17293015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inactivated influenza H5N1 whole-virus vaccine with aluminum adjuvant induces homologous and heterologous protective immunities against lethal challenge with highly pathogenic H5N1 avian influenza viruses in a mouse model. AU - Ninomiya,Ai, AU - Imai,Masaki, AU - Tashiro,Masato, AU - Odagiri,Takato, Y1 - 2007/01/30/ PY - 2006/09/21/received PY - 2006/12/22/revised PY - 2007/01/18/accepted PY - 2007/2/13/pubmed PY - 2007/6/30/medline PY - 2007/2/13/entrez SP - 3554 EP - 60 JF - Vaccine JO - Vaccine VL - 25 IS - 18 N2 - In response to recent outbreaks of H5N1 highly pathogenic avian influenza virus (HPAIV), the development of an effective H5N1 influenza vaccine is urgently important. We assessed the efficacy of two inactivated H5N1 whole-virus vaccines, rgHK213/03 and rgVNJP1203/04, generated by reverse genetics in a mouse model in the presence or absence of aluminum hydroxide (alum) adjuvant. Mice immunized with rgHK213/03 vaccine produced sufficient levels of serum antibodies that were cross-reactive to recent heterologous HPAIV-H5N1 virus, A/Turkey/12/06. The vaccinated mice also elicited protective immunity against challenge with both homologous and heterologous HPAIV-H5N1 viruses. These immune responses were enhanced by addition of alum adjuvant, resulting in antigen sparing of vaccine. On the other hand, mice immunized with rgVNJP1203/04 vaccine had low levels of serum antibodies and less protective immunity than that elicited with rgHK213/03 vaccine regardless of addition of alum adjuvant. Our study suggests that rgHK213/03 vaccine is still useful as a backup vaccine for recent H5N1 viruses and that if rgVNJP1203/04 vaccine is employed, more vaccine antigen would be necessary to induce sufficient immunity. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/17293015/Inactivated_influenza_H5N1_whole_virus_vaccine_with_aluminum_adjuvant_induces_homologous_and_heterologous_protective_immunities_against_lethal_challenge_with_highly_pathogenic_H5N1_avian_influenza_viruses_in_a_mouse_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(07)00112-0 DB - PRIME DP - Unbound Medicine ER -