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Restraint-induced corticosterone secretion and hypothalamic CRH mRNA expression are augmented during acute withdrawal from chronic cocaine administration.
Neurosci Lett 2007; 415(3):269-73NL

Abstract

Stress responses during cocaine withdrawal likely contribute to drug relapse and may be intensified as a consequence of prior cocaine use. The present study examined changes in stressor-induced activation of the hypothalamic-pituitary-adrenal (HPA) axis during acute withdrawal from chronic cocaine administration. Adult male Sprague-Dawley rats received daily administration of cocaine (30 mg/kg, i.p.) or saline for 14 days. Twenty-four hours after the last injection, rats in each group were sacrificed under stress-free conditions or following 30 min of immobilization. Plasma corticosterone (CORT) was measured in trunk-blood using radioimmunoassay, corticotropin-releasing hormone (CRH) mRNA levels in the paraventricular nucleus (PVN) of the hypothalamus were measured using in situ hybridization and glucocorticoid receptor (GR) protein expression in the pituitary gland and dissected brain regions was measured using Western blot analysis. Basal CRH mRNA in the PVN was unaltered as a result of prior cocaine administration. However, a significant increase in CRH mRNA was observed 90 min following the termination of restraint in cocaine withdrawn, but not saline-treated, rats. Basal CORT was also unaffected by prior cocaine administration, but the CORT response measured immediately after restraint was significantly augmented in cocaine-withdrawn rats. Differences in GR protein expression in number of regions implicated in negative feedback regulation of HPA function, including the hypothalamus, were not observed. These findings indicate that the HPA response to stressors is intensified during early withdrawal from cocaine administration and may be independent of changes in GR-mediated negative feedback.

Authors+Show Affiliations

Department of Biomedical Sciences, Marquette University, Milwaukee, WI 53201, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17293045

Citation

Mantsch, John R., et al. "Restraint-induced Corticosterone Secretion and Hypothalamic CRH mRNA Expression Are Augmented During Acute Withdrawal From Chronic Cocaine Administration." Neuroscience Letters, vol. 415, no. 3, 2007, pp. 269-73.
Mantsch JR, Taves S, Khan T, et al. Restraint-induced corticosterone secretion and hypothalamic CRH mRNA expression are augmented during acute withdrawal from chronic cocaine administration. Neurosci Lett. 2007;415(3):269-73.
Mantsch, J. R., Taves, S., Khan, T., Katz, E. S., Sajan, T., Tang, L. C., ... Ziegler, D. R. (2007). Restraint-induced corticosterone secretion and hypothalamic CRH mRNA expression are augmented during acute withdrawal from chronic cocaine administration. Neuroscience Letters, 415(3), pp. 269-73.
Mantsch JR, et al. Restraint-induced Corticosterone Secretion and Hypothalamic CRH mRNA Expression Are Augmented During Acute Withdrawal From Chronic Cocaine Administration. Neurosci Lett. 2007 Mar 30;415(3):269-73. PubMed PMID: 17293045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Restraint-induced corticosterone secretion and hypothalamic CRH mRNA expression are augmented during acute withdrawal from chronic cocaine administration. AU - Mantsch,John R, AU - Taves,Sarah, AU - Khan,Tayyiba, AU - Katz,Eric S, AU - Sajan,Tanveer, AU - Tang,Lee C, AU - Cullinan,William E, AU - Ziegler,Dana R, Y1 - 2007/01/21/ PY - 2006/11/02/received PY - 2007/01/05/revised PY - 2007/01/17/accepted PY - 2007/2/13/pubmed PY - 2007/6/2/medline PY - 2007/2/13/entrez SP - 269 EP - 73 JF - Neuroscience letters JO - Neurosci. Lett. VL - 415 IS - 3 N2 - Stress responses during cocaine withdrawal likely contribute to drug relapse and may be intensified as a consequence of prior cocaine use. The present study examined changes in stressor-induced activation of the hypothalamic-pituitary-adrenal (HPA) axis during acute withdrawal from chronic cocaine administration. Adult male Sprague-Dawley rats received daily administration of cocaine (30 mg/kg, i.p.) or saline for 14 days. Twenty-four hours after the last injection, rats in each group were sacrificed under stress-free conditions or following 30 min of immobilization. Plasma corticosterone (CORT) was measured in trunk-blood using radioimmunoassay, corticotropin-releasing hormone (CRH) mRNA levels in the paraventricular nucleus (PVN) of the hypothalamus were measured using in situ hybridization and glucocorticoid receptor (GR) protein expression in the pituitary gland and dissected brain regions was measured using Western blot analysis. Basal CRH mRNA in the PVN was unaltered as a result of prior cocaine administration. However, a significant increase in CRH mRNA was observed 90 min following the termination of restraint in cocaine withdrawn, but not saline-treated, rats. Basal CORT was also unaffected by prior cocaine administration, but the CORT response measured immediately after restraint was significantly augmented in cocaine-withdrawn rats. Differences in GR protein expression in number of regions implicated in negative feedback regulation of HPA function, including the hypothalamus, were not observed. These findings indicate that the HPA response to stressors is intensified during early withdrawal from cocaine administration and may be independent of changes in GR-mediated negative feedback. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/17293045/Restraint_induced_corticosterone_secretion_and_hypothalamic_CRH_mRNA_expression_are_augmented_during_acute_withdrawal_from_chronic_cocaine_administration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(07)00076-6 DB - PRIME DP - Unbound Medicine ER -