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Determination of dimethindene enantiomers in pharmaceuticals by capillary electrophoresis with carboxyethyl-beta-cyclodextrin.
Pharmazie. 2007 Jan; 62(1):31-3.P

Abstract

Cyclodextrin-mediated capillary zone electrophoresis in a hydrodynamically closed separation system with suppressed electroosmotic flow and UV absorbance photometric detection was developed for a high effective separation and quantitation of dimethindene enantiomers in various pharmaceutical formulations (solution, gel, capsules). The running buffer consisted of epsilon-aminocaproic acid (20 mmol/l) adjusted to pH 4.5 with acetic acid, negatively charged carboxyethyl-beta-cyclodextrin (2.5 mg/ml), serving as chiral selector, and 0.1% (w/v) methylhydroxyethylcellulose, serving as an electroosmotic flow suppressor. The proposed method was successfully validated appraising parameters of sensitivity, linearity, precision, accuracy/recovery and robustness and it is useful for routine use.

Authors+Show Affiliations

Mikus P
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic. mikus@fpharm.uniba.sk
Kubacák P
No affiliation info available
Valásková I
No affiliation info available
Havránek E
No affiliation info available

MeSH

Chemistry, PharmaceuticalDimethindeneElectrophoresis, CapillaryExcipientsIndicators and ReagentsReference StandardsReproducibility of ResultsStereoisomerismbeta-Cyclodextrins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17294809

Citation

Mikus, P, et al. "Determination of Dimethindene Enantiomers in Pharmaceuticals By Capillary Electrophoresis With Carboxyethyl-beta-cyclodextrin." Die Pharmazie, vol. 62, no. 1, 2007, pp. 31-3.
Mikus P, Kubacák P, Valásková I, et al. Determination of dimethindene enantiomers in pharmaceuticals by capillary electrophoresis with carboxyethyl-beta-cyclodextrin. Pharmazie. 2007;62(1):31-3.
Mikus, P., Kubacák, P., Valásková, I., & Havránek, E. (2007). Determination of dimethindene enantiomers in pharmaceuticals by capillary electrophoresis with carboxyethyl-beta-cyclodextrin. Die Pharmazie, 62(1), 31-3.
Mikus P, et al. Determination of Dimethindene Enantiomers in Pharmaceuticals By Capillary Electrophoresis With Carboxyethyl-beta-cyclodextrin. Pharmazie. 2007;62(1):31-3. PubMed PMID: 17294809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of dimethindene enantiomers in pharmaceuticals by capillary electrophoresis with carboxyethyl-beta-cyclodextrin. AU - Mikus,P, AU - Kubacák,P, AU - Valásková,I, AU - Havránek,E, PY - 2007/2/14/pubmed PY - 2007/3/14/medline PY - 2007/2/14/entrez SP - 31 EP - 3 JF - Die Pharmazie JO - Pharmazie VL - 62 IS - 1 N2 - Cyclodextrin-mediated capillary zone electrophoresis in a hydrodynamically closed separation system with suppressed electroosmotic flow and UV absorbance photometric detection was developed for a high effective separation and quantitation of dimethindene enantiomers in various pharmaceutical formulations (solution, gel, capsules). The running buffer consisted of epsilon-aminocaproic acid (20 mmol/l) adjusted to pH 4.5 with acetic acid, negatively charged carboxyethyl-beta-cyclodextrin (2.5 mg/ml), serving as chiral selector, and 0.1% (w/v) methylhydroxyethylcellulose, serving as an electroosmotic flow suppressor. The proposed method was successfully validated appraising parameters of sensitivity, linearity, precision, accuracy/recovery and robustness and it is useful for routine use. SN - 0031-7144 UR - https://www.unboundmedicine.com/medline/citation/17294809/Determination_of_dimethindene_enantiomers_in_pharmaceuticals_by_capillary_electrophoresis_with_carboxyethyl_beta_cyclodextrin_ DB - PRIME DP - Unbound Medicine ER -